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[179449_at]

Description ZK218.4 /REP_DB=WormBase Gene ID /WP=CE16704 /TR=O45999 /GB=CAB04985.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 21      6grp_total > 31      4grp_total > 46      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 154
(3)
P 		80.7
(-0.7)
P 		35
(-3)
P 		46.5
(-2.4)
A 		119
B 133.1
(3)
P 		36.1
(-2)
P 		29.2
(-2.4)
A 		17.5
(-3)
A 		116
C 138.6
(3)
P 		90.9
(0.5)
P 		54.8
(-1.4)
A 		25.3
(-3)
A 		113
avr 141.9
(3) 		69.2
(-0.9) 		39.7
(-2.5) 		29.8
(-3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 189.9
(2.1)
P 		198.5
(2.4)
P 		216.2
(3)
P 		207
(2.7)
P 		59.4
(-2.2)
P 		35
(-3)
A 		181
B 202.4
(2.4)
P 		220.9
(3)
P 		207.2
(2.6)
P 		181.1
(1.7)
P 		82.1
(-1.6)
P 		37.9
(-3)
P 		183
C 187.7
(1.3)
P 		241.2
(3)
P 		224.3
(2.5)
P 		158.1
(0.3)
P 		62.4
(-2.7)
P 		53.4
(-3)
P 		188
avr 193.3
(2.1) 		220.2
(3) 		215.9
(2.9) 		182.1
(1.7) 		68
(-2.1) 		42.1
(-3) 		178
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 56.1
(3)
P 		17.5
(-1.7)
P 		9.1
(-2.7)
P 		6.8
(-3)
A 		49
B 42.4
(3)
P 		8.9
(-2.2)
P 		6.8
(-2.5)
A 		3.7
(-3)
A 		39
C 45.9
(3)
P 		26.1
(0.1)
P 		19.1
(-0.9)
A 		4.3
(-3)
A 		42
avr 48.1
(3) 		17.5
(-1.3) 		11.7
(-2.1) 		4.9
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 35
(-0.4)
P 		70.6
(3)
P 		47.1
(0.7)
P 		52.5
(1.3)
P 		17.9
(-2.1)
P 		8.5
(-3)
A 		62
B 70.4
(2.7)
P 		73.5
(3)
P 		50.3
(0.7)
P 		48
(0.4)
P 		33.3
(-1.1)
P 		14.1
(-3)
P 		59
C 69.6
(2.6)
P 		73.6
(3)
P 		57.1
(1.2)
P 		31.8
(-1.5)
P 		20.2
(-2.8)
P 		17.9
(-3)
P 		56
avr 58.3
(1.5) 		72.6
(3) 		51.5
(0.9) 		44.1
(0.1) 		23.8
(-2) 		13.5
(-3) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179449_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13571
Sequence Type Exemplar sequence
Representative Public ID ZK218.4 NCBI
Target Description ZK218.4 /REP_DB=WormBase Gene ID /WP=CE16704 /TR=O45999 /GB=CAB04985.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074931(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074931 NCBI Caenorhabditis elegans ZK218.4 (ZK218.4) mRNA, complete cds. 11/11 None
GENEFINDER00000041077 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17101997:17104560:1 11/11 None
ZK218.4 ENSEMBL cdna:known chromosome:CEL140:V:17102299:17102942:1 gene:ZK218.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041063 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17102298-17102942(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK218.4
Entrez Gene 191244 Entrez gene
SwissProt O45999 EMBL-EBI
WormBase CE16704 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04986 Hypothetical protein ZK218.5 [Caenorhabditis elegans] ref|NP_507333.1| ZK218.5 [Caenorhabditis elegans] 1.0E-150
blast CAB04982 Hypothetical protein ZK218.3 [Caenorhabditis elegans] ref|NP_507331.1| ZK218.3 [Caenorhabditis elegans] 1.0E-108
blast CAB04987 Hypothetical protein ZK218.7 [Caenorhabditis elegans] ref|NP_507335.1| ZK218.7 [Caenorhabditis elegans] 1.0E-106
blast CAB04985 Hypothetical protein ZK218.4 [Caenorhabditis elegans] ref|NP_507332.1| ZK218.4 [Caenorhabditis elegans] 5.0E-85
blast AAB63931 Hypothetical protein ZC132.9 [Caenorhabditis elegans] ref|NP_504193.1| ZC132.9 [Caenorhabditis elegans] 6.0E-30
blast CAE62237 Hypothetical protein CBG06291 [Caenorhabditis briggsae] 5.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.9E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507332.1 3 51-73,88-110,130-152
ZK218.4 3 51-73,88-110,130-152
GENEFINDER00000041077 5 41-63,73-92,115-137,152-174,194-216
Sequence
Target Sequence 
>C. ELEGANS:179449_AT
atgttatccgtgtcaatcaagtttatatttgtgggtcacatttattcgatcacctatgaa
acttggcttggcttactttcgattgaccgatttttgagttcacgggaatcagcggaaccc
aatagatttttgactcaaagaagccttacagtattttatctgttatttatttttgtcacg
gccaaagaattaggattttatctttggatttcaatagtttccgaagactctgataagaac
aaactgttgcaagtgattttctattactacacaagctacattttcttgcaaataattttg
ttcattggaatgatatttcaattcttgatgaaatcagagtctcaagatcaacttactcgt
caaacaaaaatcattggagcaaccaagcttggcttgtttgttctattcctcttgggtatt
gtgacaggattcgtttatgttagtacaatcttcgcatctatcgacttcattttggtcccg
tcggtcattatgttaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTATCCGTGTCAATCAAGTTTA 607 47 13 Antisense
GTGGGTCACATTTATTCGATCACCT 594 487 43 Antisense
GAAACTTGGCTTGGCTTACTTTCGA 332 357 70 Antisense
CGATTGACCGATTTTTGAGTTCACG 42 249 92 Antisense
GAGTTCACGGGAATCAGCGGAACCC 667 397 108 Antisense
GATTTTCTATTACTACACAAGCTAC 594 429 267 Antisense
TTGGCTTGTTTGTTCTATTCCTCTT 143 709 401 Antisense
CTATTCCTCTTGGGTATTGTGACAG 556 223 415 Antisense
GTACAATCTTCGCATCTATCGACTT 401 461 455 Antisense
CTATCGACTTCATTTTGGTCCCGTC 706 223 470 Antisense
GGTCCCGTCGGTCATTATGTTAACA 219 467 486 Antisense
Sequence Source Affymetrix Proprietary Database