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[179447_at]

Description F14H3.9 /REP_DB=WormBase Gene ID /WP=CE15833 /TR=O45369 /GB=CAB05489.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 11      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 45.8
(-2.1)
A 		45.2
(-2.1)
A 		25.5
(-3)
A 		154.6
(3)
P 		129
B 67.2
(-1)
P 		64.9
(-1.3)
P 		52
(-3)
A 		97.5
(3)
P 		46
C 53.2
(-1.5)
P 		45.6
(-1.9)
P 		20.4
(-3)
A 		155.9
(3)
P 		136
avr 55.4
(-1.7) 		51.9
(-1.9) 		32.6
(-3) 		136
(3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 58.7
(1.5)
P 		41.7
(0.1)
A 		76.3
(3)
P 		36.2
(-0.4)
A 		40.3
(-0)
P 		4.6
(-3)
A 		72
B 56.7
(1.4)
P 		71.3
(3)
P 		42
(-0.2)
A 		16.4
(-3)
A 		58
(1.5)
P 		37.4
(-0.7)
A 		55
C 44.8
(-2.3)
A 		87
(3)
P 		68.3
(0.7)
A 		50.6
(-1.6)
A 		58.1
(-0.6)
A 		39.2
(-3)
A 		48
avr 53.4
(1) 		66.7
(3) 		62.2
(2.3) 		34.4
(-1.9) 		52.1
(0.8) 		27.1
(-3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 16.7
(0.8)
A 		9.8
(-1.8)
A 		6.7
(-3)
A 		22.6
(3)
P 		16
B 21.4
(3)
P 		16
(-0.5)
P 		12.1
(-3)
A 		20.8
(2.6)
P 		9
C 17.6
(0.3)
P 		13.1
(-1.1)
P 		7.1
(-3)
A 		26.3
(3)
P 		19
avr 18.6
(1.1) 		13
(-1.2) 		8.6
(-3) 		23.2
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 10.8
(0.8)
P 		14.8
(2.3)
A 		16.6
(3)
P 		9.2
(0.1)
A 		12.2
(1.3)
P 		1.1
(-3)
A 		16
B 19.7
(1.8)
P 		23.7
(3)
P 		10.2
(-1.2)
A 		4.4
(-3)
A 		23.6
(3)
P 		14
(-0)
A 		19
C 16.6
(-0.6)
A 		26.5
(3)
P 		17.4
(-0.3)
A 		10.2
(-3)
A 		18.8
(0.2)
A 		13.1
(-1.9)
A 		16
avr 15.7
(0.4) 		21.7
(3) 		14.7
(-0) 		7.9
(-3) 		18.2
(1.5) 		9.4
(-2.3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179447_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13214
Sequence Type Exemplar sequence
Representative Public ID F14H3.9 NCBI
Target Description F14H3.9 /REP_DB=WormBase Gene ID /WP=CE15833 /TR=O45369 /GB=CAB05489.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074642(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074642 NCBI Caenorhabditis elegans F14H3.9 (F14H3.9) mRNA, complete cds. 11/11 None
F14H3.9 ENSEMBL cdna:known chromosome:CEL140:V:16070532:16071346:-1 gene:F14H3.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000013843 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16070531-16071346(-) 96.33 100.0
Public Domain and Genome References
Gene Title
Ensembl F14H3.9
Entrez Gene 184494 Entrez gene
SwissProt O45369 EMBL-EBI
WormBase CE15833 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05489 Hypothetical protein F14H3.9 [Caenorhabditis elegans] ref|NP_507043.1| F14H3.9 [Caenorhabditis elegans] 1.0E-116
blast AAK68612 Hypothetical protein ZK105.5 [Caenorhabditis elegans] ref|NP_504996.2| ZK105.5 [Caenorhabditis elegans] 4.0E-44
blast AAA97994 Hypothetical protein K03B4.6 [Caenorhabditis elegans] ref|NP_504258.1| K03B4.6 [Caenorhabditis elegans] 5.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 5.2
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 0.0017
Sequence
Target Sequence 
>C. ELEGANS:179447_AT
ctcgtccttgtgtccaagttgcttattgatgatatcagtgatcaattagaagatggaaag
acatgcttttcggatactataagaaacatttgtgaaaagctaaaaacagaaaatactacg
gagaattacaacgcatttcttgataatattcccacaacacctgctaatgagtcggattgc
tatgatcctttttattttttcaagcatttacgatatagccagtttggagataaagctcat
agaatgttggacgattatcggaactcgagaaataatgctgatatagtggaaataaagttg
gtttttgttggatgtgatttcatttatttgaaacaaactccattcattgcctgtttgtca
aagctccgttcttctaacttcaatatatttaaatacaaatgcccgagttatgttttgcaa
cccaatgatactgcatttttgacaagcatgtttacaattgaaaaatggtgttacaaatgg
gttatgaggaagcattgtggcgaatccgctgttgcgaactttcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCGTCCTTGTGTCCAAGTTGCTTA 96 233 19 Antisense
AAGACATGCTTTTCGGATACTATAA 520 153 76 Antisense
TGATAATATTCCCACAACACCTGCT 495 565 159 Antisense
AACACCTGCTAATGAGTCGGATTGC 23 137 174 Antisense
GCTATGATCCTTTTTATTTTTTCAA 693 303 197 Antisense
AAACTCCATTCATTGCCTGTTTGTC 280 127 353 Antisense
TTGCCTGTTTGTCAAAGCTCCGTTC 322 701 365 Antisense
GCTCCGTTCTTCTAACTTCAATATA 415 299 381 Antisense
GCAACCCAATGATACTGCATTTTTG 544 319 435 Antisense
GAGGAAGCATTGTGGCGAATCCGCT 437 405 504 Antisense
CGAATCCGCTGTTGCGAACTTTCGG 97 253 519 Antisense
Sequence Source Affymetrix Proprietary Database