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[179443_s_at] --- /// 7TM receptor

Description F22B8.3 /REP_DB=WormBase Gene ID /WP=CE15881 /TR=O17831 /GB=CAB05494.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 11      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 11.3
(-2)
A 		31.2
(0.5)
A 		3.9
(-3)
A 		50.6
(3)
A 		47
B 11.1
(-1.9)
A 		6.2
(-2.9)
A 		5.5
(-3)
A 		36
(3)
A 		31
C 4.1
(-3)
A 		4.1
(-3)
A 		4.7
(-2.9)
A 		37.2
(3)
A 		33
avr 8.8
(-2.3) 		13.8
(-1.5) 		4.7
(-3) 		41.3
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 5.5
(-2.1)
A 		18.8
(3)
A 		4.6
(-2.4)
A 		4.5
(-2.5)
A 		3.1
(-3)
A 		4.7
(-2.4)
A 		16
B 19.2
(1.8)
A 		2.9
(-3)
A 		7.9
(-1.5)
A 		23.3
(3)
A 		9.2
(-1.1)
A 		9.7
(-1)
A 		20
C 5.6
(2.3)
A 		1.6
(-3)
A 		4.7
(1.1)
A 		4.5
(0.9)
A 		6.1
(3)
A 		4.8
(1.3)
A 		5
avr 10.1
(2.2) 		7.8
(-0.5) 		5.7
(-3) 		10.8
(3) 		6.1
(-2.5) 		6.4
(-2.2) 		5
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 4.1
(-0.1)
A 		6.8
(2.4)
A 		1
(-3)
A 		7.4
(3)
A 		6
B 3.5
(-0.9)
A 		1.5
(-2.8)
A 		1.3
(-3)
A 		7.7
(3)
A 		6
C 1.4
(-2.8)
A 		1.2
(-3)
A 		1.6
(-2.5)
A 		6.3
(3)
A 		5
avr 3
(-1.2) 		3.2
(-1) 		1.3
(-3) 		7.1
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 1
(-2.9)
A 		6.7
(3)
A 		1
(-2.9)
A 		1.2
(-2.7)
A 		0.9
(-3)
A 		1.1
(-2.8)
A 		6
B 6.7
(3)
A 		1
(-3)
A 		1.9
(-2.1)
A 		6.2
(2.5)
A 		3.7
(-0.2)
A 		3.6
(-0.3)
A 		6
C 2.1
(3)
A 		0.5
(-3)
A 		1.2
(-0.4)
A 		0.9
(-1.5)
A 		2
(2.6)
A 		1.6
(1.1)
A 		2
avr 3.3
(3) 		2.7
(1.1) 		1.4
(-3) 		2.8
(1.4) 		2.2
(-0.5) 		2.1
(-0.8) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179443_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13965
Sequence Type Exemplar sequence
Representative Public ID F22B8.3 NCBI
Target Description F22B8.3 /REP_DB=WormBase Gene ID /WP=CE15881 /TR=O17831 /GB=CAB05494.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074649(11), NM_074656(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074649 NCBI Caenorhabditis elegans F22B8.3 (F22B8.3) mRNA, complete cds. 11/11 A 
NM_074656 NCBI Caenorhabditis elegans Seven TM Receptor family member (str-25) (str-25) mRNA, complete cds. 11/11 A 
GENEFINDER00000002261 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16093568:16099849:1 11/11 None
GENEFINDER00000003427 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16118264:16119480:1 11/11 None
F22B8.3 ENSEMBL cdna:known chromosome:CEL140:V:16093568:16094700:1 gene:F22B8.3 11/11 A 
F44G3.11 ENSEMBL cdna:known chromosome:CEL140:V:16118264:16119480:1 gene:F44G3.11 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16093567-16094700(+) 100.0 100.0
Public Domain and Genome References
Gene Title
7TM receptor
Gene Symbol str-25
Ensembl F22B8.3
F44G3.11
Entrez Gene 184822 Entrez gene
185759 Entrez gene
SwissProt O17831 EMBL-EBI
O62240 EMBL-EBI
WormBase CE15881 Wormbase
CE16042 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05522 Hypothetical protein F44G3.11 [Caenorhabditis elegans] ref|NP_507057.1| Seven TM Receptor family member (str-25) [Caenorhabditis elegans] 0.0
blast CAB05497 Hypothetical protein F22B8.7 [Caenorhabditis elegans] ref|NP_507051.2| F22B8.7 [Caenorhabditis elegans] 0.0
blast AAB42288 Hypothetical protein F53E10.1 [Caenorhabditis elegans] ref|NP_503716.1| F53E10.1 [Caenorhabditis elegans] 1.0E-171
blast CAB05494 Hypothetical protein F22B8.3 [Caenorhabditis elegans] ref|NP_507050.1| F22B8.3 [Caenorhabditis elegans] 1.0E-168
blast CAB05494 Hypothetical protein F22B8.3 [Caenorhabditis elegans] ref|NP_507050.1| F22B8.3 [Caenorhabditis elegans] 1.0E-166
blast CAB05494 Hypothetical protein F22B8.3 [Caenorhabditis elegans] ref|NP_507050.1| F22B8.3 [Caenorhabditis elegans] 1.0E-163
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 2.5E-6
Pfam IPR005303 EMBL-EBI MOSC N-terminal beta barrel domain 3.4E-56
Pfam IPR005302 EMBL-EBI MOSC domain 3.2E-25
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 2.5E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507050.1 8 21-43,53-75,101-123,138-160,181-198,208-230,257-279,289-311
NP_507057.1 8 21-43,53-75,101-123,138-160,197-214,224-246,273-295,305-327
F22B8.3 8 21-43,53-75,101-123,138-160,181-198,208-230,257-279,289-311
F44G3.11 8 21-43,53-75,101-123,138-160,197-214,224-246,273-295,305-327
GENEFINDER00000002261 9 21-43,53-75,101-123,138-160,181-198,208-230,257-279,289-311,335-354
GENEFINDER00000003427 8 21-43,53-75,101-123,138-160,181-198,208-230,257-279,289-311
Sequence
Target Sequence 
>C. ELEGANS:179443_S_AT
tttgctctctgtccaatttgtctatcgatattgggcggtttttagtgaatcaaaactgaa
atttttcaaaggttggaggttaatattttgtatcatgtattgcgcagcttttggagcaga
ttggggattgtcaatttattattttgatgaaatggatgcctacgccgatcattatttcag
agccgaaatgcataaaagatatcggttaaacattagcgaaattgcgggcttttctctggt
tgcctacaactttgataatacccctagatggcgtaacatatcatgcacaatttctctatt
ttgtattatgcttattcaatacacaataataatttattgtgcaacaattatgtatctgaa
aatggaagaaaaattgaaattattatccttcgcaataagaaatctgcacagacaattcta
taaaacattggtaatccaaatattcactccgaccattttcctattttctcccttgatttt
tataattttccatccactcttcaacgtggaaattgttgtgccaactggaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGCTCTCTGTCCAATTTGTCTAT 156 669 354 Antisense
GCTCTCTGTCCAATTTGTCTATCGA 422 299 357 Antisense
ATTTTGTATCATGTATTGCGCAGCT 157 15 438 Antisense
ATGTATTGCGCAGCTTTTGGAGCAG 650 47 448 Antisense
TTTTGGAGCAGATTGGGGATTGTCA 159 677 462 Antisense
TACAACTTTGATAATACCCCTAGAT 168 643 598 Antisense
AATACCCCTAGATGGCGTAACATAT 523 129 610 Antisense
TGGTAATCCAAATATTCACTCCGAC 24 553 782 Antisense
ATCCAAATATTCACTCCGACCATTT 266 41 787 Antisense
TATTTTCTCCCTTGATTTTTATAAT 675 661 815 Antisense
GGAAATTGTTGTGCCAACTGGAATG 132 527 861 Antisense
Sequence Source Affymetrix Proprietary Database