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[179442_at]

Description F22B8.3 /REP_DB=WormBase Gene ID /WP=CE15881 /TR=O17831 /GB=CAB05494.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 28      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 2.6
(-2.5)
A 		1.3
(-2.9)
A 		1.1
(-3)
A 		18.9
(3)
A 		18
B 16.3
(1.9)
A 		19.3
(3)
A 		3.5
(-2.8)
A 		3
(-3)
A 		16
C 2.4
(-2.5)
A 		3.2
(-1.5)
A 		2
(-3)
A 		6.7
(3)
A 		5
avr 7.1
(1) 		7.9
(1.7) 		2.2
(-3) 		9.5
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 1.9
(-3)
A 		3.6
(-2.5)
A 		22
(3)
A 		6.2
(-1.7)
A 		3.1
(-2.6)
A 		3.1
(-2.6)
A 		20
B 0.6
(-3)
A 		22.2
(3)
A 		1.8
(-2.7)
A 		1.8
(-2.7)
A 		0.7
(-3)
A 		2.2
(-2.6)
A 		22
C 2.4
(-2.9)
A 		15.2
(3)
A 		2.5
(-2.8)
A 		4.3
(-2)
A 		2.1
(-3)
A 		2.1
(-3)
A 		13
avr 1.6
(-3) 		13.7
(3) 		8.8
(0.6) 		4.1
(-1.8) 		2
(-2.8) 		2.5
(-2.6) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 0.9
(-1.6)
A 		0.3
(-3)
A 		0.3
(-3)
A 		2.8
(3)
A 		3
B 5.2
(3)
A 		4.8
(2.5)
A 		0.8
(-2.7)
A 		0.6
(-3)
A 		5
C 0.8
(-1.5)
A 		0.9
(0)
A 		0.7
(-3)
A 		1.1
(3)
A 		0
avr 2.3
(3) 		2
(1.9) 		0.6
(-3) 		1.5
(0.2) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 0.3
(-3)
A 		1.3
(-1.7)
A 		4.8
(3)
A 		1.6
(-1.3)
A 		0.9
(-2.2)
A 		0.8
(-2.3)
A 		5
B 0.2
(-3)
A 		7.4
(3)
A 		0.4
(-2.8)
A 		0.5
(-2.8)
A 		0.3
(-2.9)
A 		0.8
(-2.5)
A 		7
C 0.9
(-2.6)
A 		4.6
(3)
A 		0.6
(-3)
A 		0.9
(-2.6)
A 		0.7
(-2.9)
A 		0.7
(-2.9)
A 		4
avr 0.5
(-3) 		4.4
(3) 		1.9
(-0.8) 		1
(-2.2) 		0.6
(-2.8) 		0.8
(-2.5) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179442_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13965
Sequence Type Exemplar sequence
Representative Public ID F22B8.3 NCBI
Target Description F22B8.3 /REP_DB=WormBase Gene ID /WP=CE15881 /TR=O17831 /GB=CAB05494.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074649(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074649 NCBI Caenorhabditis elegans F22B8.3 (F22B8.3) mRNA, complete cds. 11/11 A 
GENEFINDER00000002261 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16093568:16099849:1 11/11 None
F22B8.3 ENSEMBL cdna:known chromosome:CEL140:V:16093568:16094700:1 gene:F22B8.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16093567-16094700(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22B8.3
Entrez Gene 184822 Entrez gene
SwissProt O17831 EMBL-EBI
WormBase CE15881 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05497 Hypothetical protein F22B8.7 [Caenorhabditis elegans] ref|NP_507051.2| F22B8.7 [Caenorhabditis elegans] 0.0
blast AAB42288 Hypothetical protein F53E10.1 [Caenorhabditis elegans] ref|NP_503716.1| F53E10.1 [Caenorhabditis elegans] 1.0E-171
blast CAB05494 Hypothetical protein F22B8.3 [Caenorhabditis elegans] ref|NP_507050.1| F22B8.3 [Caenorhabditis elegans] 1.0E-168
blast CAB05494 Hypothetical protein F22B8.3 [Caenorhabditis elegans] ref|NP_507050.1| F22B8.3 [Caenorhabditis elegans] 1.0E-166
blast CAB05522 Hypothetical protein F44G3.11 [Caenorhabditis elegans] ref|NP_507057.1| Seven TM Receptor family member (str-25) [Caenorhabditis elegans] 1.0E-151
blast CAE66311 Hypothetical protein CBG11561 [Caenorhabditis briggsae] 5.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR005303 EMBL-EBI MOSC N-terminal beta barrel domain 3.4E-56
Pfam IPR005302 EMBL-EBI MOSC domain 3.2E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507050.1 8 21-43,53-75,101-123,138-160,181-198,208-230,257-279,289-311
F22B8.3 8 21-43,53-75,101-123,138-160,181-198,208-230,257-279,289-311
GENEFINDER00000002261 9 21-43,53-75,101-123,138-160,181-198,208-230,257-279,289-311,335-354
Sequence
Target Sequence 
>C. ELEGANS:179442_AT
cctacgccgatcattatttcagagccgaaatgcataaaagatatcggttaaacattagcg
aaattgcgggcttttctctggttgcctacaactttgataatacccctagatggcgtaaca
tatcatgcacaatttctctattttgtattatgcttattcaatacacaataataatttatt
gtgcaacaattatgtatctgaaaatggaagaaaaattgaaattattatccttcgcaataa
gaaatctgcacagacaattctataaaacattggtaatccaaatattcactccgaccattt
tcctattttctcccttgatttttataattttccatccactcttcaacgtggaaattgttg
tgccaactggaatgtttttatgtgcaattacaatttatccagcggcggatgccattgtta
tcatgtatgttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTACGCCGATCATTATTTCAGAGC 50 269 512 Antisense
CGCCGATCATTATTTCAGAGCCGAA 321 257 516 Antisense
GTTAAACATTAGCGAAATTGCGGGC 591 1 558 Antisense
CGAAATTGCGGGCTTTTCTCTGGTT 266 249 570 Antisense
AAATTGAAATTATTATCCTTCGCAA 604 115 724 Antisense
ATCCTTCGCAATAAGAAATCTGCAC 52 39 738 Antisense
AACTGGAATGTTTTTATGTGCAATT 352 143 876 Antisense
GCAATTACAATTTATCCAGCGGCGG 34 323 895 Antisense
TCCAGCGGCGGATGCCATTGTTATC 254 589 909 Antisense
GGCGGATGCCATTGTTATCATGTAT 395 541 915 Antisense
ATGCCATTGTTATCATGTATGTTGT 551 41 920 Antisense
Sequence Source Affymetrix Proprietary Database