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[179424_at]

Description F27C8.4 /REP_DB=WormBase Gene ID /WP=CE03269 /TR=Q19837 /GB=CAA92462.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 34      4grp_high_8_8 > 49      6grp_highest_nonorm > 15      6grp_total > 32      4grp_total > 62      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3139
1 2 3 4 range
A 5006.2
(3)
P 		2797.6
(-1.5)
P 		3567.4
(0.1)
P 		2048.7
(-3)
P 		2958
B 3740
(3)
P 		3223.4
(1.2)
P 		3165.3
(1)
P 		2025.1
(-3)
P 		1715
C 4203.7
(3)
P 		2595.6
(-1.1)
P 		3356.9
(0.8)
P 		1866.9
(-3)
P 		2337
avr 4316.6
(3) 		2872.2
(-0.7) 		3363.2
(0.6) 		1980.2
(-3) 		2336

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7778
1 2 3 4 5 6 range
A 6001.5
(0.2)
P 		6230.8
(0.4)
P 		9459.1
(3)
P 		6524.5
(0.6)
P 		3442.4
(-1.9)
P 		2145.4
(-3)
P 		7314
B 6666.9
(1.4)
P 		6918.5
(1.6)
P 		8365.8
(3)
P 		5246.8
(0)
P 		3841.9
(-1.3)
P 		2046.4
(-3)
P 		6319
C 6562.3
(1.5)
P 		6835.8
(1.7)
P 		8236.4
(3)
P 		5798.1
(0.8)
P 		3774.8
(-1.1)
P 		1681.6
(-3)
P 		6555
avr 6410.2
(1) 		6661.7
(1.2) 		8687.1
(3) 		5856.5
(0.5) 		3686.4
(-1.5) 		1957.8
(-3) 		6729
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1523
1 2 3 4 range
A 1822.8
(3)
P 		607.3
(-1.8)
P 		932.6
(-0.5)
P 		299.9
(-3)
P 		1523
B 1192.6
(3)
P 		796.1
(-0.1)
P 		733.5
(-0.6)
P 		431.6
(-3)
P 		761
C 1391.5
(3)
P 		746.2
(-0.6)
P 		1170
(1.8)
P 		314.8
(-3)
P 		1077
avr 1469
(3) 		716.5
(-1) 		945.4
(0.2) 		348.8
(-3) 		1120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1910
1 2 3 4 5 6 range
A 1106.3
(-0.9)
P 		2216
(3)
P 		2060
(2.4)
P 		1654.8
(1)
P 		1038.8
(-1.2)
P 		522.3
(-3)
P 		1694
B 2319.8
(3)
P 		2302.9
(2.9)
P 		2031.5
(1.9)
P 		1389.7
(-0.6)
P 		1560.9
(0.1)
P 		763.1
(-3)
P 		1557
C 2432.4
(3)
P 		2085.8
(1.9)
P 		2096.3
(1.9)
P 		1166
(-1.1)
P 		1221.5
(-0.9)
P 		562.9
(-3)
P 		1870
avr 1952.8
(2.1) 		2201.6
(3) 		2062.6
(2.5) 		1403.5
(-0) 		1273.7
(-0.5) 		616.1
(-3) 		1586
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179424_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10290
Sequence Type Exemplar sequence
Representative Public ID F27C8.4 NCBI
Target Description F27C8.4 /REP_DB=WormBase Gene ID /WP=CE03269 /TR=Q19837 /GB=CAA92462.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069303(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069303 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-18) (spp-18) mRNA, complete cds. 11/11 None
SNAP00000022609 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9593704:9594320:1 11/11 None
GENEFINDER00000022616 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9593701:9594320:1 11/11 None
F27C8.4 ENSEMBL cdna:known chromosome:CEL140:IV:9593704:9594320:1 gene:F27C8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9593709-9594326(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-18
Ensembl F27C8.4
Entrez Gene 185015 Entrez gene
SwissProt Q19837 EMBL-EBI
WormBase CE03269 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92462 Hypothetical protein F27C8.4 [Caenorhabditis elegans] ref|NP_501704.1| SaPosin-like Protein family member (spp-18) [Caenorhabditis elegans] 2.0E-69
blast CAE72786 Hypothetical protein CBG20048 [Caenorhabditis briggsae] 1.0E-58
blast CAE72786 Hypothetical protein CBG20048 [Caenorhabditis briggsae] 6.0E-58
Sequence
Target Sequence 
>C. ELEGANS:179424_AT
tcgtggtcccacaaaatgctgatcaatgtggagccgttgatatcacaaagatggcaaaaa
gatatgttccatctcaaaactctgacgccacctgcgaaatctgtcttgatcttgttctga
ttgccgagacttatgccgaatgcgacgaagctatcgtgcaacatcacatggatgcttatt
gtgttgaacacgtcaagaatcatgcttcccaggcactttgcaagcttttaattgacgata
ttgctcatgccgtcattgaagataccgatcaaaatccaactggggtctgtcaaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGTGGTCCCACAAAATGCTGATCA 25 607 65 Antisense
TGTTCCATCTCAAAACTCTGACGCC 206 563 129 Antisense
GCCACCTGCGAAATCTGTCTTGATC 21 281 151 Antisense
GTCTTGATCTTGTTCTGATTGCCGA 413 467 167 Antisense
GATTGCCGAGACTTATGCCGAATGC 651 433 183 Antisense
ATGCCGAATGCGACGAAGCTATCGT 468 41 197 Antisense
GAAGCTATCGTGCAACATCACATGG 666 341 211 Antisense
ACGTCAAGAATCATGCTTCCCAGGC 409 93 254 Antisense
TTCCCAGGCACTTTGCAAGCTTTTA 472 693 270 Antisense
GACGATATTGCTCATGCCGTCATTG 321 379 298 Antisense
AAATCCAACTGGGGTCTGTCAAAAG 531 117 336 Antisense
Sequence Source Affymetrix Proprietary Database