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[179423_at]

Description R12G8.2 /REP_DB=WormBase Gene ID /WP=CE20028 /TR=Q9XTV6 /GB=CAB07854.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 86      4grp_total > 41      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 11
(-3)
A 		17
(3)
A 		15.9
(1.9)
A 		13.7
(-0.3)
A 		6
B 7.8
(-3)
A 		16.4
(3)
A 		11.5
(-0.4)
A 		8.3
(-2.7)
A 		9
C 3.2
(-3)
A 		9.5
(-0.3)
A 		17
(3)
A 		16.4
(2.7)
A 		14
avr 7.3
(-3) 		14.3
(2.6) 		14.8
(3) 		12.8
(1.4) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 5.1
(-2.7)
A 		6.7
(-2.3)
A 		3.6
(-3)
A 		5.5
(-2.6)
A 		13.9
(-0.7)
A 		30
(3)
A 		26
B 5.8
(-1.2)
A 		5.4
(-1.7)
A 		5.9
(-1)
A 		9
(3)
A 		4.4
(-3)
A 		5.8
(-1.2)
A 		5
C 6.9
(-1.9)
A 		5.1
(-2.8)
A 		4.8
(-3)
A 		16.7
(3)
A 		6.6
(-2.1)
A 		5.2
(-2.8)
A 		12
avr 5.9
(-2.3) 		5.7
(-2.4) 		4.8
(-3) 		10.4
(0.8) 		8.3
(-0.6) 		13.7
(3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 4
(2.5)
A 		3.7
(1.6)
A 		4.2
(3)
A 		2
(-3)
A 		2
B 2.5
(-1.2)
A 		4.1
(3)
A 		2.7
(-0.7)
A 		1.8
(-3)
A 		2
C 1.1
(-3)
A 		2.7
(-1)
A 		5.9
(3)
A 		2.8
(-0.9)
A 		5
avr 2.5
(-2.1) 		3.5
(0.7) 		4.3
(3) 		2.2
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 0.9
(-2.9)
A 		2.4
(-1.5)
A 		0.8
(-3)
A 		1.4
(-2.4)
A 		4.2
(0.1)
A 		7.3
(3)
A 		7
B 2
(0.6)
A 		1.8
(-0.6)
A 		1.4
(-3)
A 		2.4
(3)
A 		1.8
(-0.6)
A 		2.2
(1.8)
A 		1
C 2.6
(1)
A 		1.6
(-1.9)
A 		1.2
(-3)
A 		3.3
(3)
A 		2.1
(-0.4)
A 		1.7
(-1.6)
A 		2
avr 1.8
(-1.4) 		1.9
(-1.2) 		1.1
(-3) 		2.4
(-0) 		2.7
(0.7) 		3.7
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179423_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14050
Sequence Type Exemplar sequence
Representative Public ID R12G8.2 NCBI
Target Description R12G8.2 /REP_DB=WormBase Gene ID /WP=CE20028 /TR=Q9XTV6 /GB=CAB07854.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075084(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075084 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-36) (twk-36) mRNA, complete cds. 11/11 None
SNAP00000026659 ENSEMBL cdna:SNAP chromosome:CEL140:V:17746227:17749630:-1 11/11 None
GENEFINDER00000026661 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17746227:17749630:-1 11/11 None
R12G8.2 ENSEMBL cdna:known chromosome:CEL140:V:17746227:17749635:-1 gene:R12G8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17746226-17749204(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-36
Ensembl R12G8.2
Entrez Gene 180164 Entrez gene
SwissProt Q9XTV6 EMBL-EBI
WormBase CE36712 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07854 Hypothetical protein R12G8.2 [Caenorhabditis elegans] ref|NP_507485.2| TWiK family of potassium channels family member (twk-36) [Caenorhabditis elegans] 0.0
blast CAE61712 Hypothetical protein CBG05661 [Caenorhabditis briggsae] 0.0
blast CAE61731 Hypothetical protein CBG05682 [Caenorhabditis briggsae] 1.0E-124
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507485.2 5 166-188,293-315,354-376,391-408,421-443
R12G8.2 5 166-188,293-315,354-376,391-408,421-443
SNAP00000026659 5 166-188,293-315,354-376,391-408,421-443
GENEFINDER00000026661 5 166-188,293-315,354-376,391-408,421-443
Sequence
Target Sequence 
>C. ELEGANS:179423_AT
atgactattctgatagcctacctcctcctctgcaccacgttcgtgtacctctacgacgcc
gttatgggcccggaatgggacgacggcctaccgtacttcaccgcattctacttctctttc
atctctctcacaactattggtctaggcgatgtgatgccaaataatgttccgtacgccccg
ccggtctcgatgatatttttcatcggcatggcagtcacaaaagttgtgaatcgagcaaca
tttattgcagtggaaaatggggtttttggcctgatgaccttggcggaaaccaagattagc
atgctcttgacggaacggaaacctggtgtaaaaactgtcagatcaacatcatcagggtct
tcagcagagtccgtggattcaaaagagccacgtggatttgatggggactcggatagtgga
tcggatgatggggtaatcaaccgccgccgaaacgagatgatgaacaccttcaccgtccga
tcaattgccacattcatgcgttccaaccatgacgtctatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGACTATTCTGATAGCCTACCTCC 237 45 859 Antisense
TACCTCTACGACGCCGTTATGGGCC 149 623 904 Antisense
TTCATCTCTCTCACAACTATTGGTC 202 689 976 Antisense
ATGCCAAATAATGTTCCGTACGCCC 147 37 1012 Antisense
GGGTTTTTGGCCTGATGACCTTGGC 591 447 1118 Antisense
GATTAGCATGCTCTTGACGGAACGG 243 429 1152 Antisense
ATCAACATCATCAGGGTCTTCAGCA 369 27 1200 Antisense
GTCTTCAGCAGAGTCCGTGGATTCA 159 469 1215 Antisense
TCAACCGCCGCCGAAACGAGATGAT 495 631 1295 Antisense
GTCCGATCAATTGCCACATTCATGC 136 475 1333 Antisense
ATGCGTTCCAACCATGACGTCTATG 512 41 1354 Antisense
Sequence Source Affymetrix Proprietary Database