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[179419_at]

Description F56A8.4 /REP_DB=WormBase Gene ID /WP=CE16124 /TR=O45575 /GB=CAB05744.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 96      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 91      6grp_total > 96      4grp_total > 01      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 191
1 2 3 4 range
A 101.8
(-2.1)
P 		88
(-3)
P 		121.2
(-0.9)
P 		183.6
(3)
P 		96
B 100.8
(-2.9)
P 		103.2
(-2.8)
P 		98.6
(-3)
P 		279
(3)
P 		180
C 104.4
(-2.4)
P 		93.7
(-3)
P 		115.2
(-1.8)
P 		205.8
(3)
P 		112
avr 102.3
(-2.7) 		95
(-3) 		111.7
(-2.2) 		222.8
(3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 268
1 2 3 4 5 6 range
A 131.6
(-1.5)
P 		93.2
(-3)
P 		172.8
(0.2)
P 		176.5
(0.3)
P 		203.6
(1.4)
P 		243.9
(3)
P 		151
B 152.4
(-3)
P 		155.8
(-2.9)
P 		178.9
(-2)
P 		216
(-0.7)
P 		273.8
(1.4)
P 		317.1
(3)
P 		165
C 113.7
(-3)
P 		161.4
(-1.8)
P 		129.8
(-2.6)
P 		267
(0.7)
P 		261.6
(0.6)
P 		361.6
(3)
P 		248
avr 132.6
(-3) 		136.8
(-2.9) 		160.5
(-2) 		219.8
(-0) 		246.3
(0.9) 		307.5
(3) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 37.1
(3)
P 		19.1
(-3)
P 		31.7
(1.2)
P 		26.9
(-0.4)
P 		18
B 32.2
(-1.5)
P 		25.5
(-2.6)
P 		22.9
(-3)
P 		59.5
(3)
P 		37
C 34.6
(0.5)
P 		26.9
(-3)
P 		40.2
(3)
P 		34.7
(0.5)
P 		13
avr 34.6
(0.9) 		23.8
(-3) 		31.6
(-0.2) 		40.4
(3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 24.3
(-3)
P 		33.1
(-1.6)
P 		37.6
(-0.8)
P 		44.8
(0.3)
P 		61.4
(3)
P 		59.4
(2.7)
P 		37
B 53
(-2.2)
P 		51.9
(-2.3)
P 		43.4
(-3)
P 		57.2
(-1.9)
P 		111.2
(2.4)
P 		118.2
(3)
P 		75
C 42.2
(-2.4)
P 		49.3
(-1.9)
P 		33
(-3)
P 		53.7
(-1.6)
P 		84.7
(0.5)
P 		121
(3)
P 		88
avr 39.8
(-2.8) 		44.8
(-2.3) 		38
(-3) 		51.9
(-1.6) 		85.8
(1.7) 		99.5
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179419_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6690
Sequence Type Exemplar sequence
Representative Public ID F56A8.4 NCBI
Target Description F56A8.4 /REP_DB=WormBase Gene ID /WP=CE16124 /TR=O45575 /GB=CAB05744.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067331(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067331 NCBI Caenorhabditis elegans F56A8.4 (F56A8.4) mRNA, complete cds. 11/11 None
SNAP00000043512 ENSEMBL cdna:SNAP chromosome:CEL140:III:13262835:13266401:1 11/11 None
GENEFINDER00000043516 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13262835:13264536:1 11/11 None
F56A8.4 ENSEMBL cdna:known chromosome:CEL140:III:13262803:13264486:1 gene:F56A8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13262830-13264482(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F56A8.4
Entrez Gene 176741 Entrez gene
SwissProt O45575 EMBL-EBI
WormBase CE16124 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05745 Hypothetical protein F56A8.5 [Caenorhabditis elegans] ref|NP_499733.1| F56A8.5 [Caenorhabditis elegans] 1.0E-164
blast CAB05744 Hypothetical protein F56A8.4 [Caenorhabditis elegans] ref|NP_499732.1| F56A8.4 [Caenorhabditis elegans] 1.0E-161
blast CAB05744 Hypothetical protein F56A8.4 [Caenorhabditis elegans] ref|NP_499732.1| F56A8.4 [Caenorhabditis elegans] 1.0E-151
blast CAB05744 Hypothetical protein F56A8.4 [Caenorhabditis elegans] ref|NP_499732.1| F56A8.4 [Caenorhabditis elegans] 1.0E-150
blast CAE60061 Hypothetical protein CBG03576 [Caenorhabditis briggsae] 7.0E-51
blast CAB05745 Hypothetical protein F56A8.5 [Caenorhabditis elegans] ref|NP_499733.1| F56A8.5 [Caenorhabditis elegans] 9.0E-34
blast CAB05745 Hypothetical protein F56A8.5 [Caenorhabditis elegans] ref|NP_499733.1| F56A8.5 [Caenorhabditis elegans] 2.0E-26
blast CAE60061 Hypothetical protein CBG03576 [Caenorhabditis briggsae] 8.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.3E-4
Sequence
Target Sequence 
>C. ELEGANS:179419_AT
aaaaagttctcctgggctttcatgccgtcgcaaacttcacggatagtaaagtactacaga
aataaaacaatttccgagacgatgtttccgtatgaaaggatgattgtctcaggcaatggt
gaaacttggagaatgcaggaattagtgaattcaagattaataaagcaagtcgacaaggtt
caattgacgaacacgtttattgctaatccatgtcgatttgaattaaaggaattggaagtt
cttcttatcaatcagtgcaattggttcactactagttctttggaagcttggaacccaaaa
tacctaaaaatcaagacaccatacttttcaagttggatgactaatctatttattcaagat
tgctgcaacttagataaaaacgggaaactaagaaggattgtattctatgggaaatacaat
aaagtccagattttggaagatttaaactcgaaaagattcgaaaaaggcagacgacaagaa
atctacccaggacctaagccgcttgactgcaaactcggatacgatatcgagcgtaaggac
ggccttctgtctacaattgtttttggacgagaacgaattgattttgttttctggcatgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAAGTTCTCCTGGGCTTTCATGC 686 129 211 Antisense
ATTTCCGAGACGATGTTTCCGTATG 310 15 280 Antisense
GACGAACACGTTTATTGCTAATCCA 656 377 396 Antisense
GAAGTTCTTCTTATCAATCAGTGCA 188 337 445 Antisense
TGCAATTGGTTCACTACTAGTTCTT 130 575 466 Antisense
GACACCATACTTTTCAAGTTGGATG 603 367 525 Antisense
AGAAATCTACCCAGGACCTAAGCCG 507 69 687 Antisense
TAAGCCGCTTGACTGCAAACTCGGA 45 637 705 Antisense
GATATCGAGCGTAAGGACGGCCTTC 700 419 733 Antisense
ACGGCCTTCTGTCTACAATTGTTTT 41 97 749 Antisense
AATTGATTTTGTTTTCTGGCATGAA 406 179 786 Antisense
Sequence Source Affymetrix Proprietary Database