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[179414_s_at]

Description R06A4.2 /REP_DB=WormBase Gene ID /WP=CE18109 /TR=O62329 /GB=CAB05583.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 79      4grp_highest_8_8 > 63      4grp_high_8_8 > 28      6grp_highest_nonorm > 91      6grp_total > 59      4grp_total > 28      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 240.2
(-0.2)
P 		239.7
(-0.2)
P 		183.3
(-3)
P 		306
(3)
P 		123
B 209.2
(2.4)
P 		216.8
(3)
P 		136.5
(-3)
P 		192.3
(1.2)
P 		80
C 216.7
(-1.1)
P 		269.3
(3)
P 		192.9
(-3)
P 		265.5
(2.7)
P 		76
avr 222
(0.7) 		241.9
(2.1) 		170.9
(-3) 		254.6
(3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 232.1
(3)
P 		152.5
(-3)
P 		199.4
(0.5)
P 		204.2
(0.9)
P 		193.4
(0.1)
P 		223.4
(2.3)
P 		80
B 248.5
(0.4)
P 		189.3
(-3)
P 		201.3
(-2.3)
P 		198.4
(-2.5)
P 		251.5
(0.6)
P 		292.8
(3)
P 		104
C 234.5
(0.5)
P 		205.2
(-1)
P 		175.7
(-2.5)
P 		166.7
(-3)
P 		240.6
(0.8)
P 		283.9
(3)
P 		117
avr 238.4
(1) 		182.3
(-3) 		192.1
(-2.3) 		189.8
(-2.5) 		228.5
(0.3) 		266.7
(3) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 87.5
(3)
P 		52
(-2)
P 		47.9
(-2.6)
P 		44.8
(-3)
P 		43
B 66.7
(3)
P 		53.5
(0.7)
P 		31.6
(-3)
P 		41
(-1.4)
P 		35
C 71.7
(2)
P 		77.4
(3)
P 		67.2
(1.1)
P 		44.8
(-3)
P 		33
avr 75.3
(3) 		61
(0.3) 		48.9
(-2) 		43.5
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 42.8
(-3)
P 		54.2
(1.4)
P 		43.4
(-2.8)
P 		51.8
(0.5)
P 		58.4
(3)
P 		54.4
(1.5)
P 		16
B 86.5
(0.7)
P 		63
(-1.6)
P 		48.9
(-3)
P 		52.6
(-2.6)
P 		102.2
(2.3)
P 		109.2
(3)
P 		60
C 86.9
(2.2)
P 		62.6
(-0.2)
P 		44.7
(-1.9)
P 		33.5
(-3)
P 		77.9
(1.3)
P 		95
(3)
P 		62
avr 72.1
(0.9) 		59.9
(-0.9) 		45.7
(-3) 		46
(-3) 		79.5
(2) 		86.2
(3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179414_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2944
Sequence Type Exemplar sequence
Representative Public ID R06A4.2 NCBI
Target Description R06A4.2 /REP_DB=WormBase Gene ID /WP=CE18109 /TR=O62329 /GB=CAB05583.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064585(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064585 NCBI Caenorhabditis elegans R06A4.2 (R06A4.2) mRNA, complete cds. 11/11 None
SNAP00000043978 ENSEMBL cdna:SNAP chromosome:CEL140:II:14361913:14364765:1 11/11 None
GENEFINDER00000043988 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14361913:14364765:1 11/11 None
R06A4.2.1 ENSEMBL cdna:known chromosome:CEL140:II:14361900:14365064:1 gene:R06A4.2 11/11 None
R06A4.2.2 ENSEMBL cdna:known chromosome:CEL140:II:14361909:14364765:1 gene:R06A4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14361909-14364762(+) 98.89 100.0
Public Domain and Genome References
Gene Title
Ensembl R06A4.2
Entrez Gene 175093 Entrez gene
SwissProt O62329 EMBL-EBI
WormBase CE18109 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05583 Hypothetical protein R06A4.2 [Caenorhabditis elegans] ref|NP_496986.1| R06A4.2 [Caenorhabditis elegans] 0.0
blast AAB03119 Hypothetical protein T12E12.3 [Caenorhabditis elegans] ref|NP_500827.1| T12E12.3 [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:179414_S_AT
gtctcatcatcatctgagccagtgtcatcagccgagccagctgccgctgcgctcgtgccg
tctgaagccggtcaaggatctcgagacgtttttacatcacaatccgactcaacgattcca
gagtcttctagctttgatgagctgcttccaaattcgtgcagggtgaaaatggagaagtca
tattccgacatcccacgatcagctgagccagtgttcaaggttcctatgataccaaatcgc
ctatcccacagcgcctccaatggtttaaagccggccgagatttctccaattttggtggat
tatgatgctctgaactcgcaagtcgttcagcaagtcttggacgatgccgacgataaagat
ttggaattgatacacgaagcgttggagaaagcttttggaaatcaggaaaattggactgac
aagtccacagcgaagaccgttacaatcggcacaattatcaaggcgattgagaccaaattc
acaggaatctctcgtgaagttcttctggaacagaaggaatccattgtggaagaggttctc
ggcaacgtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTCATCATCATCTGAGCCAGTGT 400 467 1897 Antisense
TCTGAGCCAGTGTCATCAGCCGAGC 343 609 1909 Antisense
GAGACGTTTTTACATCACAATCCGA 673 391 1979 Antisense
TGATGAGCTGCTTCCAAATTCGTGC 578 565 2031 Antisense
TCCCACGATCAGCTGAGCCAGTGTT 601 597 2087 Antisense
GAGCCAGTGTTCAAGGTTCCTATGA 201 383 2101 Antisense
TATGATACCAAATCGCCTATCCCAC 77 657 2121 Antisense
ACAGCGCCTCCAATGGTTTAAAGCC 262 107 2144 Antisense
GATGCTCTGAACTCGCAAGTCGTTC 290 413 2200 Antisense
AGTCTTGGACGATGCCGACGATAAA 686 61 2229 Antisense
GTGGAAGAGGTTCTCGGCAACGTCG 534 491 2422 Antisense
Sequence Source Affymetrix Proprietary Database