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[179407_at]

Description R12H7.4 /REP_DB=WormBase Gene ID /WP=CE03569 /TR=Q21967 /GB=CAA90634.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 03      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 36.9
(-2.8)
A 		54.5
(-1.9)
A 		33.7
(-3)
A 		143.6
(3)
P 		110
B 81.7
(2)
M 		39.6
(-1.8)
A 		26.8
(-3)
A 		92.1
(3)
A 		65
C 53.3
(-3)
M 		61.1
(-2.6)
A 		67.2
(-2.4)
A 		183.7
(3)
P 		130
avr 57.3
(-2.1) 		51.7
(-2.4) 		42.6
(-3) 		139.8
(3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 87.8
(1.6)
A 		71.4
(0.4)
M 		105.8
(3)
P 		70.5
(0.3)
M 		85.1
(1.4)
A 		26.2
(-3)
A 		80
B 88.3
(0.9)
P 		118.6
(3)
P 		94
(1.3)
P 		43
(-2.2)
A 		53.6
(-1.5)
A 		32.1
(-3)
A 		87
C 63.9
(-1)
A 		133.2
(3)
P 		111.6
(1.8)
P 		103.3
(1.3)
A 		28.3
(-3)
A 		40.1
(-2.3)
A 		105
avr 80
(0.8) 		107.7
(3) 		103.8
(2.7) 		72.3
(0.2) 		55.7
(-1.2) 		32.8
(-3) 		75
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 13.4
(-0.7)
A 		11.8
(-1.5)
A 		8.8
(-3)
A 		21
(3)
P 		12
B 26
(3)
M 		9.8
(-1.9)
A 		6.2
(-3)
A 		19.6
(1.1)
A 		20
C 17.6
(-3)
M 		17.6
(-3)
A 		23.4
(-0.4)
A 		31
(3)
P 		13
avr 19
(0.4) 		13.1
(-2.8) 		12.8
(-3) 		23.9
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 16.2
(0)
A 		25.4
(2.9)
M 		23
(2.2)
P 		17.9
(0.6)
M 		25.7
(3)
A 		6.4
(-3)
A 		19
B 30.7
(1.1)
P 		39.5
(3)
P 		22.8
(-0.6)
P 		11.4
(-3)
A 		21.8
(-0.8)
A 		12
(-2.9)
A 		28
C 23.7
(-0.2)
A 		40.7
(3)
P 		28.4
(0.7)
P 		20.8
(-0.8)
A 		9.1
(-3)
A 		13.4
(-2.2)
A 		32
avr 23.5
(0.1) 		35.2
(3) 		24.7
(0.4) 		16.7
(-1.5) 		18.9
(-1) 		10.6
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179407_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17540
Sequence Type Exemplar sequence
Representative Public ID R12H7.4 NCBI
Target Description R12H7.4 /REP_DB=WormBase Gene ID /WP=CE03569 /TR=Q21967 /GB=CAA90634.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077789(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077789 NCBI Caenorhabditis elegans R12H7.4 (R12H7.4) mRNA, complete cds. 11/11 None
GENEFINDER00000017900 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13217057:13218851:1 11/11 None
SNAP00000017894 ENSEMBL cdna:SNAP chromosome:CEL140:X:13217668:13218851:1 11/11 None
R12H7.4 ENSEMBL cdna:known chromosome:CEL140:X:13217057:13218851:1 gene:R12H7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13217056-13218851(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12H7.4
Entrez Gene 187847 Entrez gene
SwissProt Q21967 EMBL-EBI
WormBase CE03569 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90634 Hypothetical protein R12H7.4 [Caenorhabditis elegans] ref|NP_510190.1| R12H7.4 [Caenorhabditis elegans] 0.0
blast CAE61398 Hypothetical protein CBG05257 [Caenorhabditis briggsae] 1.0E-152
blast CAE61398 Hypothetical protein CBG05257 [Caenorhabditis briggsae] 1.0E-146
blast CAA90634 Hypothetical protein R12H7.4 [Caenorhabditis elegans] ref|NP_510190.1| R12H7.4 [Caenorhabditis elegans] 1.0E-139
blast CAE61398 Hypothetical protein CBG05257 [Caenorhabditis briggsae] 4.0E-99
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510190.1 5 51-73,111-133,148-170,200-219,224-246
R12H7.4 5 51-73,111-133,148-170,200-219,224-246
GENEFINDER00000017900 7 51-73,85-107,117-139,152-174,189-211,241-260,265-287
SNAP00000017894 4 13-35,40-62,82-104,109-131
Sequence
Target Sequence 
>C. ELEGANS:179407_AT
gctgggctgaaagcgcaactagcgtgtattgcatgcgaccggcaactaccatctcaagat
atttatcaatgctattgggcgagaagatcagtaaaatcattggttttgatttcgcagtat
atattcagcatttggcttatgatattatttgctgcagaacaccagaaactctacttgttt
attctggtcaccttcatgacactcctatttggaccagctctttttgtttgccacacatat
ggacatttgaatacctgtcagaagtgggctggttcggggtgttttgctagtttatacgct
atgtgtccaccgaaaccttcaacatatgaaaaagataaagatgatgaagaagatgagaag
attgaaaatcctcaggaaaaaacacctctagaaaatgaagatgaaaatccagaaaagcaa
cctggagctggcgactcgaaagagccagatgagacatcaggaaatagtagtccgaaaaaa
tcaacacaaagaggaaaaggactggaccccacaaagcacacagagtctcaaaaattctac
tcatggctcaccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGGCTGAAAGCGCAACTAGCGT 701 293 514 Antisense
CTAGCGTGTATTGCATGCGACCGGC 436 223 532 Antisense
ACCGGCAACTACCATCTCAAGATAT 265 89 551 Antisense
TACTTGTTTATTCTGGTCACCTTCA 559 643 685 Antisense
TGACACTCCTATTTGGACCAGCTCT 690 571 710 Antisense
AGCTCTTTTTGTTTGCCACACATAT 513 79 729 Antisense
CAGAAGTGGGCTGGTTCGGGGTGTT 541 151 772 Antisense
TGTCCACCGAAACCTTCAACATATG 208 557 817 Antisense
AAAAGCAACCTGGAGCTGGCGACTC 316 129 926 Antisense
GACCCCACAAAGCACACAGAGTCTC 703 381 1018 Antisense
AAAAATTCTACTCATGGCTCACCGA 436 129 1043 Antisense
Sequence Source Affymetrix Proprietary Database