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[179393_at]

Description F31D4.7 /REP_DB=WormBase Gene ID /WP=CE15933 /TR=O45422 /GB=CAB07375.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 12      4grp_total > 62      6grp_cgc > 42      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 64.7
(3)
A 		9.2
(-2.8)
A 		37.7
(0.2)
A 		7.4
(-3)
A 		57
B 38.1
(3)
A 		26
(1)
A 		18.4
(-0.3)
A 		2.6
(-3)
A 		36
C 62.4
(3)
A 		50.3
(1.6)
A 		34.3
(-0.3)
A 		10.7
(-3)
A 		52
avr 55.1
(3) 		28.5
(-0.3) 		30.1
(-0.1) 		6.9
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 44.9
(-0.3)
A 		40.2
(-1)
A 		69.4
(3)
A 		25.4
(-3)
A 		61.1
(1.9)
A 		35.2
(-1.7)
A 		44
B 74.4
(3)
A 		55.6
(0.9)
A 		47.2
(-0.1)
A 		45.6
(-0.3)
A 		21.4
(-3)
A 		26.7
(-2.4)
A 		53
C 50
(-0.4)
A 		110.3
(3)
A 		40.6
(-1)
A 		23.1
(-2)
A 		22.8
(-2)
A 		5.2
(-3)
A 		105
avr 56.4
(1.4) 		68.7
(3) 		52.4
(0.9) 		31.4
(-1.8) 		35.1
(-1.4) 		22.4
(-3) 		46
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 23.6
(3)
A 		2
(-2.8)
A 		9.8
(-0.7)
A 		1.1
(-3)
A 		23
B 12.1
(3)
A 		6.4
(0.1)
A 		4.3
(-1)
A 		0.5
(-3)
A 		12
C 20.7
(3)
A 		14.5
(1)
A 		12
(0.2)
A 		1.8
(-3)
A 		19
avr 18.8
(3) 		7.6
(-0.8) 		8.7
(-0.4) 		1.1
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 8.3
(-2.1)
A 		14.3
(1)
A 		15.1
(1.4)
A 		6.4
(-3)
A 		18.4
(3)
A 		8.6
(-1.9)
A 		12
B 25.9
(3)
A 		18.5
(0.4)
A 		11.5
(-2)
A 		12.1
(-1.8)
A 		8.7
(-3)
A 		9.9
(-2.6)
A 		17
C 18.5
(0.2)
A 		33.7
(3)
A 		10.3
(-1.4)
A 		4.6
(-2.5)
A 		7.4
(-1.9)
A 		1.7
(-3)
A 		32
avr 17.6
(1.2) 		22.2
(3) 		12.3
(-0.8) 		7.7
(-2.6) 		11.5
(-1.1) 		6.7
(-3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179393_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14752
Sequence Type Exemplar sequence
Representative Public ID F31D4.7 NCBI
Target Description F31D4.7 /REP_DB=WormBase Gene ID /WP=CE15933 /TR=O45422 /GB=CAB07375.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075630(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075630 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-35) (twk-35) mRNA, complete cds. 11/11 None
SNAP00000041986 ENSEMBL cdna:SNAP chromosome:CEL140:V:20861032:20863794:1 11/11 None
F31D4.7 ENSEMBL cdna:known chromosome:CEL140:V:20861032:20863794:1 gene:F31D4.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000041996 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20861751-20863794(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-35
Ensembl F31D4.7
Entrez Gene 185149 Entrez gene
SwissProt O45422 EMBL-EBI
WormBase CE34006 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07375 Hypothetical protein F31D4.7 [Caenorhabditis elegans] ref|NP_508031.2| TWiK family of potassium channels family member (twk-35) [Caenorhabditis elegans] 0.0
blast CAE61631 Hypothetical protein CBG05561 [Caenorhabditis briggsae] 0.0
blast CAE67712 Hypothetical protein CBG13286 [Caenorhabditis briggsae] 4.0E-41
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508031.2 6 145-164,239-261,273-295,339-361,368-390,394-416
F31D4.7 6 145-164,239-261,273-295,339-361,368-390,394-416
SNAP00000041986 6 145-164,239-261,273-295,339-361,368-390,394-416
Sequence
Target Sequence 
>C. ELEGANS:179393_AT
aactggacacttctcgaatcattctattttttcttcacatctctgagtaccgtcggtctt
ggagacctggttccttcttctccgcgactgttaattacaatgtttggattcatcttggtt
ggattgtcgctagtatccatggttatcaacttgttgcaagcaaagatgaaatcaacttac
gaagctggccgaaatgatgaaaaaacaccacatattcatcaaaccttacccacctcactt
ggagttctgcaatgtttctccccagacgaagaaaaaaagactgattactcagaaagaagt
ttgagtagatctactcaaacgagtttgagccttcctggcgtgagacaggtcaataacaca
gcaagtatcaatttgagattattactattgcaggtagtcttacgatcagacggcgtgcat
tgggtgaacacagatacctcatcacctacgaaatcacctgatgaagtcaccaacctcgtg
gagtatgagacggctctgcgtgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTGGACACTTCTCGAATCATTCT 287 143 718 Antisense
TTCACATCTCTGAGTACCGTCGGTC 361 685 751 Antisense
GTCGGTCTTGGAGACCTGGTTCCTT 675 415 769 Antisense
TTCTTCTCCGCGACTGTTAATTACA 64 689 792 Antisense
ATTGTCGCTAGTATCCATGGTTATC 347 5 840 Antisense
ACCACATATTCATCAAACCTTACCC 338 83 924 Antisense
TGGAGTTCTGCAATGTTTCTCCCCA 636 549 957 Antisense
AAACGAGTTTGAGCCTTCCTGGCGT 284 125 1034 Antisense
GATACCTCATCACCTACGAAATCAC 381 425 1150 Antisense
CACCTGATGAAGTCACCAACCTCGT 501 201 1172 Antisense
GGAGTATGAGACGGCTCTGCGTGTC 205 517 1197 Antisense
Sequence Source Affymetrix Proprietary Database