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[179375_at] sterol reductase

Description B0250.9 /REP_DB=WormBase Gene ID /WP=CE19655 /TR=Q9XVF2 /GB=CAB03797.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 10      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 71      6grp_total > 03      4grp_total > 46      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 467
1 2 3 4 range
A 615.7
(3)
P
302.4
(-1.6)
P
204.4
(-3)
P
294.2
(-1.7)
P
411
B 652.5
(3)
P
267.1
(-2)
P
187
(-3)
P
216.4
(-2.6)
P
466
C 593.1
(3)
P
259.7
(-1.9)
P
185.9
(-3)
P
222.7
(-2.5)
P
407
avr 620.4
(3)
276.4
(-1.8)
192.4
(-3)
244.4
(-2.3)
428

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 321
1 2 3 4 5 6 range
A 469
(3)
P
302.9
(-0.1)
P
341.3
(0.6)
P
330.5
(0.4)
P
362.7
(1)
P
148.3
(-3)
P
321
B 405.8
(3)
P
376.4
(2.3)
P
316
(0.9)
P
392.4
(2.7)
P
344.3
(1.6)
P
151.3
(-3)
P
255
C 344.5
(1)
P
424.5
(2.8)
P
325.7
(0.6)
P
435.7
(3)
P
312.9
(0.4)
P
155.3
(-3)
P
280
avr 406.4
(3)
367.9
(2.1)
327.7
(1.1)
386.2
(2.5)
340
(1.4)
151.6
(-3)
255
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 187
1 2 3 4 range
A 224.2
(3)
P
65.6
(-2.3)
P
53.4
(-2.7)
P
43.1
(-3)
P
181
B 208.1
(3)
P
66
(-2.2)
P
43.3
(-3)
P
46.1
(-2.9)
P
165
C 196.3
(3)
P
74.7
(-1.6)
P
64.8
(-2)
P
37.6
(-3)
P
159
avr 209.5
(3)
68.8
(-2)
53.8
(-2.6)
42.3
(-3)
167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 86.4
(1.1)
P
107.7
(2.9)
P
74.3
(0.1)
P
83.8
(0.9)
P
109.4
(3)
P
36.1
(-3)
P
73
B 141.2
(3)
P
125.3
(1.9)
P
76.7
(-1.6)
P
103.9
(0.4)
P
139.9
(2.9)
P
56.4
(-3)
P
85
C 127.7
(2.9)
P
129.5
(3)
P
82.9
(-0.6)
P
87.6
(-0.2)
P
101.3
(0.8)
P
52
(-3)
P
78
avr 118.4
(2.8)
120.8
(3)
78
(-0.5)
91.8
(0.6)
116.9
(2.7)
48.2
(-3)
73
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179375_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13845
Sequence Type Exemplar sequence
Representative Public ID B0250.9 NCBI
Target Description B0250.9 /REP_DB=WormBase Gene ID /WP=CE19655 /TR=Q9XVF2 /GB=CAB03797.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075540(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_075540 NCBI Caenorhabditis elegans B0250.9 (B0250.9) mRNA, complete cds. 11/11 None
SNAP00000043591 ENSEMBL cdna:SNAP chromosome:CEL140:V:20478248:20481535:-1 11/11 None
GENEFINDER00000043601 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20478948:20484703:-1 11/11 None
B0250.9 ENSEMBL cdna:known chromosome:CEL140:V:20478844:20483436:-1 gene:B0250.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043583 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20478947-20487253(-) 100.0 100.0
Public Domain and Genome References
Gene Title sterol reductase
Ensembl B0250.9
Entrez Gene 181886 Entrez gene
SwissProt Q9XVF2 EMBL-EBI
WormBase CE36579 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03797 Hypothetical protein B0250.9 [Caenorhabditis elegans] ref|NP_507941.2| B0250.9 [Caenorhabditis elegans] gb|AAQ14296.1| sterol reductase [Caenorhabditis elegans] 0.0
blast CAE61655 Hypothetical protein CBG05589 [Caenorhabditis briggsae] 0.0
blast CAE61655 Hypothetical protein CBG05589 [Caenorhabditis briggsae] 1.0E-164
blast CAB03797 Hypothetical protein B0250.9 [Caenorhabditis elegans] ref|NP_507941.2| B0250.9 [Caenorhabditis elegans] gb|AAQ14296.1| sterol reductase [Caenorhabditis elegans] 1.0E-121
blast CAE61655 Hypothetical protein CBG05589 [Caenorhabditis briggsae] 1.0E-106
blast AAN15564 sterol delta7 reductase [Arabidopsis thaliana] gb|AAF63498.1| sterol delta7 reductase [Arabidopsis thaliana] gb|AAM20440.1| sterol delta7 reductase [Arabidopsis thaliana] gb|AAF87888.1| sterol delta7 reductase [Arabidopsis thaliana] ref|NP_175460.1| 7-dehydrocholesterol reductase / 7-DHC reductase / sterol delta-7-reductase (ST7R) / dwarf5 protein (DWF5) [Arabidopsis thaliana] pir||F96540 sterol delta7 reductase [imported] - Arabidopsis thaliana sp|Q9LDU6|ST7R_ARATH 7-dehydrocholesterol reductase (7-DHC reductase) (Sterol delta-7-reductase) (Dwarf5 protein) 4.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR001171 EMBL-EBI ERG4/ERG24 ergosterol biosynthesis protein 8.1E-9
Pfam IPR001171 EMBL-EBI ERG4/ERG24 ergosterol biosynthesis protein 8.1E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507941.2 8 48-70,135-157,164-186,220-237,244-266,286-308,315-334,405-427
B0250.9 8 48-70,135-157,164-186,220-237,244-266,286-308,315-334,405-427
SNAP00000043591 6 7-26,30-52,59-81,101-123,130-149,217-239
GENEFINDER00000043601 7 199-221,228-250,284-301,308-330,350-372,379-398,469-491
Sequence
Target Sequence
>C. ELEGANS:179375_AT
ttatcaagcgtccaactactctacattttccaatttcactggaacgaagacctctttctc
aactcgttggactcgaagcgatgcgatttcggcttctatcgaatttgggcggatttcgtg
ctgggacccattatctatacatcaccagtgacgattctcgtggcgactaatcgatccgtc
ggtgtgatctccaactgcttattctgtgctgtggcgattggctcgatggttttcactgcg
aaatgcgatcggcagaaatatgaattccggaaatctaaaggaacgctgaaagttggcgga
gttgatgctttttttattagtgcaaagtatcgaactgactccggagatgctaatactaat
cttttactaggcagtggtcattggggagtatgcaggcatccaaattatgcctccgaagcc
atcacattcgccgctttctctgcatttcaaggcttcccatccaccattgctcacatccca
tccttattcgttatcctgttccttgttgcacgagcattcacc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
TTATCAAGCGTCCAACTACTCTACA 86 681 1495 Antisense
ACCTCTTTCTCAACTCGTTGGACTC 477 87 1544 Antisense
GCGATGCGATTTCGGCTTCTATCGA 486 289 1572 Antisense
GGGACCCATTATCTATACATCACCA 480 493 1617 Antisense
ACCAGTGACGATTCTCGTGGCGACT 34 87 1638 Antisense
AATCGATCCGTCGGTGTGATCTCCA 109 169 1663 Antisense
GATCTCCAACTGCTTATTCTGTGCT 704 417 1680 Antisense
TATTCTGTGCTGTGGCGATTGGCTC 106 661 1694 Antisense
CGATTGGCTCGATGGTTTTCACTGC 127 249 1709 Antisense
TTTCTCTGCATTTCAAGGCTTCCCA 386 671 1929 Antisense
GTTCCTTGTTGCACGAGCATTCACC 577 439 1992 Antisense
Sequence Source Affymetrix Proprietary Database