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[179373_at]

Description C50F4.9 /REP_DB=WormBase Gene ID /WP=CE05473 /TR=Q18744 /GB=CAA94744.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 08      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 80      6grp_total > 18      4grp_total > 47      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 366
1 2 3 4 range
A 327.9
(3)
P 		101
(-3)
P 		108.4
(-2.8)
P 		119.3
(-2.5)
P 		227
B 346.9
(3)
P 		110.6
(-2)
P 		65.2
(-3)
P 		101.9
(-2.2)
P 		282
C 426.2
(3)
P 		108.5
(-2.2)
P 		60
(-3)
P 		152.5
(-1.5)
P 		366
avr 367
(3) 		106.7
(-2.4) 		77.9
(-3) 		124.6
(-2) 		289

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 273.2
(3)
P 		132
(-3)
P 		154.9
(-2)
P 		200.6
(-0.1)
P 		149.6
(-2.3)
P 		187.3
(-0.7)
P 		141
B 252.5
(3)
P 		248.2
(2.8)
P 		109
(-3)
P 		169.3
(-0.5)
P 		209.7
(1.2)
P 		159.5
(-0.9)
P 		144
C 227.8
(2.4)
P 		202.7
(0.9)
P 		133.5
(-3)
P 		239.2
(3)
P 		203.5
(1)
P 		164.2
(-1.3)
P 		106
avr 251.2
(3) 		194.3
(0.1) 		132.5
(-3) 		203
(0.6) 		187.6
(-0.2) 		170.3
(-1.1) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 119.4
(3)
P 		21.9
(-2.7)
P 		28.3
(-2.4)
P 		17.5
(-3)
P 		102
B 110.6
(3)
P 		27.3
(-2.2)
P 		15.1
(-3)
P 		21.7
(-2.6)
P 		96
C 141.1
(3)
P 		31.2
(-2.5)
P 		20.9
(-3)
P 		25.7
(-2.8)
P 		120
avr 123.7
(3) 		26.8
(-2.7) 		21.4
(-3) 		21.6
(-3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 50.4
(2.8)
P 		46.9
(1.6)
P 		33.7
(-3)
P 		50.9
(3)
P 		45.1
(1)
P 		45.6
(1.2)
P 		17
B 87.9
(3)
P 		82.6
(2.5)
P 		26.5
(-3)
P 		44.8
(-1.2)
P 		85.2
(2.7)
P 		59.5
(0.2)
P 		61
C 84.4
(3)
P 		61.8
(0.3)
P 		34
(-3)
P 		48.1
(-1.3)
P 		65.9
(0.8)
P 		55
(-0.5)
P 		50
avr 74.2
(3) 		63.8
(1.5) 		31.4
(-3) 		47.9
(-0.7) 		65.4
(1.8) 		53.4
(0.1) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179373_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12418
Sequence Type Exemplar sequence
Representative Public ID C50F4.9 NCBI
Target Description C50F4.9 /REP_DB=WormBase Gene ID /WP=CE05473 /TR=Q18744 /GB=CAA94744.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073053(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073053 NCBI Caenorhabditis elegans C50F4.9 (C50F4.9) mRNA, complete cds. 11/11 None
SNAP00000009307 ENSEMBL cdna:SNAP chromosome:CEL140:V:9525894:9526268:-1 11/11 None
GENEFINDER00000009325 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9525894:9526783:-1 10/11 None
C50F4.9 ENSEMBL cdna:known chromosome:CEL140:V:9525894:9526268:-1 gene:C50F4.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9525893-9526268(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C50F4.9
Entrez Gene 183680 Entrez gene
SwissProt Q18744 EMBL-EBI
WormBase CE05473 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94744 Hypothetical protein C50F4.9 [Caenorhabditis elegans] ref|NP_505454.1| C50F4.9 [Caenorhabditis elegans] 6.0E-58
blast CAA94744 Hypothetical protein C50F4.9 [Caenorhabditis elegans] ref|NP_505454.1| C50F4.9 [Caenorhabditis elegans] 6.0E-54
blast CAE72188 Hypothetical protein CBG19296 [Caenorhabditis briggsae] 2.0E-53
blast CAE72188 Hypothetical protein CBG19296 [Caenorhabditis briggsae] 6.0E-45
Sequence
Target Sequence 
>C. ELEGANS:179373_AT
ttcatttcaggttcaatcgttgctattctgaccgatcaaattgaagaaagtacggcctgt
aatctgaagcatctgctcagaaatgatccacttttcaagccactcattcaaactaatgtg
cacaaaaagagaagcgcctatgaaatcattggagtacacacatgcgaggagaatgagcca
tgcagaatcgggagaactcgttcatgcttgtgccaagaagattttgtatgtgacaaaaca
cctgccaacaaaaacgtacatctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCATTTCAGGTTCAATCGTTGCTA 332 687 22 Antisense
TCGTTGCTATTCTGACCGATCAAAT 646 603 38 Antisense
GGCCTGTAATCTGAAGCATCTGCTC 635 543 75 Antisense
GCATCTGCTCAGAAATGATCCACTT 640 309 90 Antisense
AAATGATCCACTTTTCAAGCCACTC 250 119 102 Antisense
TTTTCAAGCCACTCATTCAAACTAA 295 675 113 Antisense
ATTGGAGTACACACATGCGAGGAGA 678 3 169 Antisense
AGCCATGCAGAATCGGGAGAACTCG 44 83 197 Antisense
AACTCGTTCATGCTTGTGCCAAGAA 607 141 216 Antisense
GTATGTGACAAAACACCTGCCAACA 466 455 247 Antisense
ACCTGCCAACAAAAACGTACATCTT 272 87 261 Antisense
Sequence Source Affymetrix Proprietary Database