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[179365_at]

Description C50B8.5 /REP_DB=WormBase Gene ID /WP=CE08885 /TR=Q18725 /GB=CAB01128.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 11      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 109
(-0.2)
A 		79.5
(-1.9)
A 		61.1
(-3)
A 		163.2
(3)
P 		102
B 72.5
(-3)
P 		111.1
(-0.9)
A 		105.5
(-1.2)
A 		183.8
(3)
P 		111
C 98.6
(-1.9)
P 		90.7
(-2.1)
A 		63.1
(-3)
A 		251.4
(3)
P 		188
avr 93.4
(-2.2) 		93.8
(-2.2) 		76.6
(-3) 		199.5
(3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 59.2
(-3)
A 		97
(0.7)
P 		120.8
(3)
P 		104.4
(1.4)
M 		81.5
(-0.8)
M 		92
(0.2)
A 		62
B 73.2
(-1.9)
P 		84.9
(-1)
A 		59.4
(-3)
A 		83.2
(-1.2)
A 		137.1
(3)
P 		100.7
(0.2)
M 		78
C 72.4
(1)
A 		87.7
(2)
P 		9.5
(-3)
A 		104.1
(3)
A 		102.3
(2.9)
P 		104
(3)
M 		95
avr 68.3
(-2.3) 		89.9
(0.7) 		63.2
(-3) 		97.2
(1.7) 		107
(3) 		98.9
(1.9) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 39.7
(3)
A 		17.3
(-2.7)
A 		16
(-3)
A 		23.9
(-1)
P 		24
B 23.1
(-3)
P 		27.4
(-1.4)
A 		24.4
(-2.5)
A 		39.2
(3)
P 		16
C 32.7
(0.1)
P 		26.1
(-1.8)
A 		22
(-3)
A 		42.4
(3)
P 		20
avr 31.8
(1.6) 		23.6
(-1.8) 		20.8
(-3) 		35.2
(3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 10.9
(-3)
A 		34.5
(3)
P 		26.3
(0.9)
P 		26.5
(1)
M 		24.6
(0.5)
M 		22.4
(-0.1)
A 		24
B 25.5
(-1.4)
P 		28.3
(-1)
A 		14.4
(-3)
A 		22
(-1.9)
A 		55.7
(3)
P 		37.5
(0.4)
M 		41
C 26.9
(1.5)
A 		26.8
(1.5)
P 		2.4
(-3)
A 		20.9
(0.4)
A 		33.1
(2.7)
P 		34.8
(3)
M 		32
avr 21.1
(-1.3) 		29.9
(1) 		14.4
(-3) 		23.1
(-0.8) 		37.8
(3) 		31.6
(1.4) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179365_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13221
Sequence Type Exemplar sequence
Representative Public ID C50B8.5 NCBI
Target Description C50B8.5 /REP_DB=WormBase Gene ID /WP=CE08885 /TR=Q18725 /GB=CAB01128.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073963
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000010255 ENSEMBL cdna:SNAP chromosome:CEL140:V:13585912:13586473:1 None
C50B8.5 ENSEMBL cdna:known chromosome:CEL140:V:13583585:13587235:1 gene:C50B8.5 None
GENEFINDER00000010259 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13583638:13586473:1 None
NM_073963 NCBI Caenorhabditis elegans C50B8.5 (C50B8.5) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13583637-13586520(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C50B8.5
Entrez Gene 179843 Entrez gene
SwissProt Q18725 EMBL-EBI
WormBase CE36920 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC70092 Hypothetical protein C50B8.5 [Caenorhabditis elegans] emb|CAB01128.3| Hypothetical protein C50B8.5 [Caenorhabditis elegans] ref|NP_506364.2| C50B8.5 [Caenorhabditis elegans] 1.0E-69
blast CAE66287 Hypothetical protein CBG11534 [Caenorhabditis briggsae] 2.0E-60
blast CAC70092 Hypothetical protein C50B8.5 [Caenorhabditis elegans] emb|CAB01128.3| Hypothetical protein C50B8.5 [Caenorhabditis elegans] ref|NP_506364.2| C50B8.5 [Caenorhabditis elegans] 1.0E-41
blast CAC70092 Hypothetical protein C50B8.5 [Caenorhabditis elegans] emb|CAB01128.3| Hypothetical protein C50B8.5 [Caenorhabditis elegans] ref|NP_506364.2| C50B8.5 [Caenorhabditis elegans] 8.0E-40
blast CAE66287 Hypothetical protein CBG11534 [Caenorhabditis briggsae] 4.0E-39
blast CAE66287 Hypothetical protein CBG11534 [Caenorhabditis briggsae] 4.0E-36
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506364.2 1 74-96
C50B8.5 1 74-96
SNAP00000010255 1 65-87
GENEFINDER00000010259 2 12-34,91-113
Sequence
Target Sequence 
>C. ELEGANS:179365_AT
acttcttcgtcatcacgttttcatttttgttttttctagtctaccaaaccgtgagaactc
attagtgacaccgtgcatttcaatacgagacttttcttttatgacgcttcatattcattt
cgttccacattttccaatttgtctcctcaatttccaaatgctttgcgtaatctatgtcgc
gaactattttttatttcagctcgaccaaggactcggtcaactcgttgacggcatgaattt
tggtctcggggttttcgaccaacattttccaagtgaatggctttacctcctcgtgctcat
gcttattgttctaatacttcttggtcttgttggttatcttctttattatattgtcaacta
cattcttaatcgacatgatagatatgaagaatatcgaatgtggtatttaaaaaatgtaat
gaaacaagatgatgtcgattgggaagaagatgatgagtttgctgaaggattttattcatt
aagtgatgacactccgccaccgacttatagtgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTCTTCGTCATCACGTTTTCATT 563 103 219 Antisense
GTGACACCGTGCATTTCAATACGAG 255 483 283 Antisense
TTGTCTCCTCAATTTCCAAATGCTT 568 707 357 Antisense
GCGTAATCTATGTCGCGAACTATTT 300 291 383 Antisense
ATTTCAGCTCGACCAAGGACTCGGT 333 15 411 Antisense
CGGCATGAATTTTGGTCTCGGGGTT 321 237 447 Antisense
CCAAGTGAATGGCTTTACCTCCTCG 235 277 487 Antisense
CCTCGTGCTCATGCTTATTGTTCTA 687 267 507 Antisense
ATTGTTCTAATACTTCTTGGTCTTG 191 5 523 Antisense
CTTGGTCTTGTTGGTTATCTTCTTT 66 219 538 Antisense
ACTCCGCCACCGACTTATAGTGAAT 674 97 709 Antisense
Sequence Source Affymetrix Proprietary Database