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[179352_at]

Description F53F8.2 /REP_DB=WormBase Gene ID /WP=CE18729 /TR=O62262 /GB=CAB04462.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 75      4grp_total > 05      6grp_cgc > 66      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 8.4
(-2.4)
A 		3
(-2.9)
A 		1.8
(-3)
A 		65.8
(3)
A 		64
B 53.5
(3)
A 		8.8
(-2.7)
A 		6.5
(-3)
A 		36.8
(0.9)
A 		47
C 21.5
(-1.7)
A 		6
(-2.9)
A 		4.3
(-3)
A 		82.5
(3)
A 		78
avr 27.8
(-0.5) 		5.9
(-2.8) 		4.2
(-3) 		61.7
(3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 26.7
(1.3)
A 		4.5
(-2.6)
A 		11
(-1.5)
A 		2.3
(-3)
A 		17.3
(-0.3)
A 		36.2
(3)
A 		34
B 5.6
(-2.4)
A 		2.7
(-3)
A 		30.2
(2.6)
A 		28.9
(2.3)
A 		20
(0.5)
A 		32.1
(3)
A 		29
C 3.9
(-2.4)
A 		22.2
(1.9)
A 		20.8
(1.6)
A 		26.9
(3)
A 		12.2
(-0.5)
A 		1.4
(-3)
A 		26
avr 12.1
(-2) 		9.8
(-3) 		20.7
(1.9) 		19.4
(1.3) 		16.5
(0) 		23.2
(3) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 3.1
(-1.3)
A 		0.6
(-2.9)
A 		0.5
(-3)
A 		9.6
(3)
A 		9
B 17
(3)
A 		2.2
(-2.7)
A 		1.5
(-3)
A 		7.8
(-0.6)
A 		16
C 7.1
(-0.3)
A 		1.7
(-2.9)
A 		1.5
(-3)
A 		13.9
(3)
A 		12
avr 9.1
(2.2) 		1.5
(-2.8) 		1.2
(-3) 		10.4
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 4.9
(0.1)
A 		1.6
(-2.3)
A 		2.4
(-1.7)
A 		0.6
(-3)
A 		5.2
(0.4)
A 		8.8
(3)
A 		8
B 1.9
(-2.5)
A 		0.9
(-3)
A 		7.3
(0.5)
A 		7.7
(0.7)
A 		8.1
(0.9)
A 		12
(3)
A 		11
C 1.4
(-2.1)
A 		6.8
(3)
A 		5.3
(1.6)
A 		5.4
(1.7)
A 		4
(0.3)
A 		0.5
(-3)
A 		6
avr 2.7
(-3) 		3.1
(-2.5) 		5
(0.1) 		4.6
(-0.4) 		5.8
(1.2) 		7.1
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179352_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13828
Sequence Type Exemplar sequence
Representative Public ID F53F8.2 NCBI
Target Description F53F8.2 /REP_DB=WormBase Gene ID /WP=CE18729 /TR=O62262 /GB=CAB04462.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075593
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000039381 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20680321:20681065:1 None
NM_075593 NCBI Caenorhabditis elegans Serpentine Receptor, class SX family member (srsx-35) (srsx-35) mRNA, complete cds. None
F53F8.2 ENSEMBL cdna:known chromosome:CEL140:V:20679471:20681065:1 gene:F53F8.2 None
SNAP00000039375 ENSEMBL cdna:SNAP chromosome:CEL140:V:20680321:20681065:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20680320-20682127(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srsx-35
Ensembl F53F8.2
Entrez Gene 186180 Entrez gene
SwissProt O62262 EMBL-EBI
WormBase CE36441 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAH10781 Hypothetical protein F53F8.2 [Caenorhabditis elegans] emb|CAB04462.2| Hypothetical protein F53F8.2 [Caenorhabditis elegans] ref|NP_507994.2| Serpentine Receptor, class SX family member (srsx-35) [Caenorhabditis elegans] 1.0E-177
blast CAB05565 Hypothetical protein M01B2.7 [Caenorhabditis elegans] ref|NP_506777.2| Serpentine Receptor, class SX family member (srsx-37) [Caenorhabditis elegans] 1.0E-146
blast CAB03426 Hypothetical protein T26E4.15 [Caenorhabditis elegans] ref|NP_506945.2| Serpentine Receptor, class SX family member (srsx-36) [Caenorhabditis elegans] 1.0E-144
blast CAH10781 Hypothetical protein F53F8.2 [Caenorhabditis elegans] emb|CAB04462.2| Hypothetical protein F53F8.2 [Caenorhabditis elegans] ref|NP_507994.2| Serpentine Receptor, class SX family member (srsx-35) [Caenorhabditis elegans] 1.0E-112
blast CAB05565 Hypothetical protein M01B2.7 [Caenorhabditis elegans] ref|NP_506777.2| Serpentine Receptor, class SX family member (srsx-37) [Caenorhabditis elegans] 2.0E-85
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507994.2 7 5-27,39-61,76-98,119-141,161-183,196-218,233-255
F53F8.2 7 5-27,39-61,76-98,119-141,161-183,196-218,233-255
SNAP00000039375 4 9-31,51-73,86-108,123-145
GENEFINDER00000039381 4 9-31,51-73,86-108,123-145
Sequence
Target Sequence 
>C. ELEGANS:179352_AT
ccgcccatctacgcatatgtgtattttaagatcaaaaatatgagaatgaatagctcaatg
aaagcagtcttcaaatcattaatggtcactgtatgccttgttgtatgtggttggatgacg
actgacttgattggtgcgcttacggtaacactgccaatggataaggacgtggcaagaatg
attcagttgtactgcggaacgtttatattcacgtcgagcgcatttaatgccgtggtgtat
tacaaattgagcccaaaacattctttcagtcgagactaccgttcagcaatgcgcacaatg
cttggtctctcgaatgaaatatccatagcaaaaggaaccacttatcaacaagctacagat
gcacagagatcaaaggatataaaaaatacctatataatagattgtagttattgtattttc
tcacttccgttgtctcaaaaaatggaacctcttctaattatccttttcgttatttattgt
acagtttttgtaattgggactactggaaatttgattatggtcctggtgacatattattgc
aaaaatctccggtcaatttgcaacgtccttattggtttgtgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGCCCATCTACGCATATGTGTATT 182 263 205 Antisense
AATGGTCACTGTATGCCTTGTTGTA 435 163 285 Antisense
TTGGTGCGCTTACGGTAACACTGCC 111 705 335 Antisense
TTCACGTCGAGCGCATTTAATGCCG 469 687 412 Antisense
GAGCCCAAAACATTCTTTCAGTCGA 37 387 453 Antisense
CGAGACTACCGTTCAGCAATGCGCA 27 251 475 Antisense
AATGCGCACAATGCTTGGTCTCTCG 14 167 492 Antisense
GTTATTGTATTTTCTCACTTCCGTT 363 447 611 Antisense
GAACCTCTTCTAATTATCCTTTTCG 512 345 649 Antisense
AATCTCCGGTCAATTTGCAACGTCC 22 171 748 Antisense
TGCAACGTCCTTATTGGTTTGTGTT 33 575 763 Antisense
Sequence Source Affymetrix Proprietary Database