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[179350_at]

Description F47B8.5 /REP_DB=WormBase Gene ID /WP=CE10640 /TR=Q20517 /GB=CAB01192.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 25      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 5
(-3)
A 		6
(-2.6)
A 		12
(-0.3)
A 		20.8
(3)
A 		16
B 6.5
(-1.9)
A 		15.8
(3)
A 		10.1
(0)
A 		4.3
(-3)
A 		12
C 5.4
(-3)
A 		14
(0.8)
A 		11.6
(-0.3)
A 		19.1
(3)
A 		14
avr 5.6
(-3) 		11.9
(1.2) 		11.2
(0.7) 		14.7
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1660
1 2 3 4 5 6 range
A 17.3
(-2.9)
A 		8.6
(-3)
A 		3.1
(-3)
A 		205.6
(-2.3)
P 		1663.1
(3)
P 		770.6
(-0.2)
P 		1660
B 11.3
(-3)
A 		13.8
(-3)
A 		9
(-3)
A 		238.2
(-2)
P 		1437.7
(3)
P 		779.6
(0.2)
P 		1429
C 11.6
(-3)
A 		51.7
(-2.8)
A 		15.9
(-3)
A 		166
(-2.4)
P 		1516.7
(3)
P 		668.3
(-0.4)
P 		1505
avr 13.4
(-3) 		24.7
(-2.9) 		9.3
(-3) 		203.3
(-2.2) 		1539.2
(3) 		739.5
(-0.1) 		1530
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 1.8
(-1.3)
A 		1.3
(-3)
A 		3.1
(3)
A 		3
(2.7)
A 		2
B 2.1
(-0.6)
A 		3.9
(3)
A 		2.3
(-0.2)
A 		0.9
(-3)
A 		3
C 1.8
(-3)
A 		4
(3)
A 		4
(3)
A 		3.2
(0.8)
A 		2
avr 1.9
(-3) 		3.1
(3) 		3.1
(3) 		2.4
(-0.5) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 583
1 2 3 4 5 6 range
A 3.2
(-3)
A 		3.1
(-3)
A 		0.7
(-3)
A 		52.1
(-2.4)
P 		501.8
(3)
P 		187.6
(-0.8)
P 		501
B 3.9
(-3)
A 		4.6
(-3)
A 		2.2
(-3)
A 		63.1
(-2.4)
P 		584.1
(3)
P 		290.7
(-0)
P 		582
C 4.3
(-3)
A 		15.8
(-2.9)
A 		4
(-3)
A 		33.4
(-2.6)
P 		490.8
(3)
P 		223.7
(-0.3)
P 		487
avr 3.8
(-3) 		7.8
(-2.9) 		2.3
(-3) 		49.5
(-2.5) 		525.6
(3) 		234
(-0.3) 		523
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179350_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13607
Sequence Type Exemplar sequence
Representative Public ID F47B8.5 NCBI
Target Description F47B8.5 /REP_DB=WormBase Gene ID /WP=CE10640 /TR=Q20517 /GB=CAB01192.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074150(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074150 NCBI Caenorhabditis elegans F47B8.5 (F47B8.5) mRNA, complete cds. 11/11 None
SNAP00000011439 ENSEMBL cdna:SNAP chromosome:CEL140:V:14327411:14330411:-1 11/11 None
GENEFINDER00000011451 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14327411:14330411:-1 11/11 None
F47B8.5 ENSEMBL cdna:known chromosome:CEL140:V:14327411:14330411:-1 gene:F47B8.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028772 1/11 Cross Hyb Matching Probes None
NM_001028773 1/11 Cross Hyb Matching Probes None
SNAP00000011435 1/11 Cross Hyb Matching Probes None
SNAP00000011436 1/11 Cross Hyb Matching Probes None
GENEFINDER00000011447 1/11 Cross Hyb Matching Probes None
GENEFINDER00000011458 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14327410-14330411(-) 91.52 100.0
Public Domain and Genome References
Gene Title
Ensembl F47B8.5
Entrez Gene 185897 Entrez gene
SwissProt Q20517 EMBL-EBI
WormBase CE10640 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01192 Hypothetical protein F47B8.5 [Caenorhabditis elegans] ref|NP_506551.1| F47B8.5 [Caenorhabditis elegans] 0.0
blast CAB01192 Hypothetical protein F47B8.5 [Caenorhabditis elegans] ref|NP_506551.1| F47B8.5 [Caenorhabditis elegans] 1.0E-150
blast CAE60920 Hypothetical protein CBG04637 [Caenorhabditis briggsae] 6.0E-87
blast CAE72711 Hypothetical protein CBG19948 [Caenorhabditis briggsae] 1.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR002485 EMBL-EBI Protein of unknown function DUF13 1.4E-54
Pfam IPR002485 EMBL-EBI Protein of unknown function DUF13 9.0E-52
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506551.1 1 20-37
F47B8.5 1 20-37
SNAP00000011439 1 20-37
GENEFINDER00000011451 1 20-37
Sequence
Target Sequence 
>C. ELEGANS:179350_AT
tgatcttcgctgtttctactttaatcttttctgctcactgtcagtattcaagtggcggag
gaggtggtggttactcaggtggtggaggtggtggaggagaagctgcaccaccatcttcac
cacctgccgatgcagcccctgctccaccaccagctccggaaccagttccagctccagctc
cagctccagaagccgcacctgttgcaccatcagctgacgctggaggatatgccgctgcgg
ctgcacccgccggtggcggatcttatccagccaagaagagaagagtagctcgtgattatg
ctgaaggagaagctgctccagctgctccagcagaacccgccccagctccagctccagctc
cagcaccagcacccgaagcggctccagcaccagaaccagcgccagctccagcaccagcac
cagcaccagaagccgctccggctgcggcaccagatgccgctccagcagaaccagctccag
tacctgaggttgctcctgcccccgcacctgctccagcagccccagaatcagatgccagtg
gttactcctctggtgctgctgctccagctggaggtggtggatcttatccagcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATCTTCGCTGTTTCTACTTTAAT 546 565 1130 Antisense
TTCTACTTTAATCTTTTCTGCTCAC 393 691 1143 Antisense
TTTTCTGCTCACTGTCAGTATTCAA 487 677 1156 Antisense
AGGTGGTGGTTACTCAGGTGGTGGA 496 57 1191 Antisense
TGTTGCACCATCAGCTGACGCTGGA 454 563 1329 Antisense
GGAGGATATGCCGCTGCGGCTGCAC 24 517 1351 Antisense
CGGTGGCGGATCTTATCCAGCCAAG 543 243 1380 Antisense
CAGCTCCAGTACCTGAGGTTGCTCC 154 203 1601 Antisense
AGCAGCCCCAGAATCAGATGCCAGT 601 77 1644 Antisense
AGATGCCAGTGGTTACTCCTCTGGT 487 67 1659 Antisense
GGAGGTGGTGGATCTTATCCAGCCA 250 517 1699 Antisense
Sequence Source Affymetrix Proprietary Database