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[179343_at]

Description B0491.1 /REP_DB=WormBase Gene ID /WP=CE02103 /TR=Q17515 /GB=CAA90090.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 97      4grp_highest_8_8 > 35      4grp_high_8_8 > 56      6grp_highest_nonorm > 73      6grp_total > 95      4grp_total > 63      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 279
1 2 3 4 range
A 654.9
(3)
P 		375.6
(-3)
P 		595.2
(1.7)
P 		474.9
(-0.9)
P 		279
B 500.3
(-1.2)
P 		580.2
(3)
P 		486.1
(-2)
P 		466.8
(-3)
P 		113
C 622.7
(3)
P 		426.9
(-2.8)
P 		529.2
(0.2)
P 		421.5
(-3)
P 		201
avr 592.6
(3) 		460.9
(-2.7) 		536.8
(0.6) 		454.4
(-3) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 579.4
(1)
P 		612.5
(2)
P 		451.5
(-3)
P 		566.4
(0.6)
P 		644.6
(3)
P 		601
(1.6)
P 		193
B 552
(0.2)
P 		570.8
(0.6)
P 		408.6
(-3)
P 		577.7
(0.7)
P 		632.9
(2)
P 		680.3
(3)
P 		272
C 436
(-3)
P 		581.8
(1.3)
P 		575.5
(1.1)
P 		641.6
(3)
P 		492.7
(-1.3)
P 		581.2
(1.2)
P 		206
avr 522.5
(-1.1) 		588.4
(1.6) 		478.5
(-3) 		595.2
(1.9) 		590.1
(1.7) 		620.8
(3) 		142
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 238.5
(3)
P 		81.5
(-2.6)
P 		155.6
(0.1)
P 		69.5
(-3)
P 		169
B 159.6
(3)
P 		143.3
(1.4)
P 		112.6
(-1.7)
P 		99.5
(-3)
P 		60
C 206.1
(3)
P 		122.7
(-0.7)
P 		184.4
(2)
P 		71.1
(-3)
P 		135
avr 201.4
(3) 		115.8
(-1.2) 		150.9
(0.5) 		80
(-3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 106.8
(-2.6)
P 		217.8
(3)
P 		98.3
(-3)
P 		143.6
(-0.7)
P 		194.5
(1.8)
P 		146.3
(-0.6)
P 		120
B 192.1
(0.5)
P 		190
(0.5)
P 		99.2
(-3)
P 		153
(-1)
P 		257.1
(3)
P 		253.7
(2.9)
P 		158
C 161.6
(-0)
P 		177.5
(1.4)
P 		146.5
(-1.4)
P 		129
(-3)
P 		159.4
(-0.2)
P 		194.6
(3)
P 		66
avr 153.5
(-0.4) 		195.1
(2.4) 		114.7
(-3) 		141.9
(-1.2) 		203.7
(3) 		198.2
(2.6) 		89
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179343_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3977
Sequence Type Exemplar sequence
Representative Public ID B0491.1 NCBI
Target Description B0491.1 /REP_DB=WormBase Gene ID /WP=CE02103 /TR=Q17515 /GB=CAA90090.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064025(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064025 NCBI Caenorhabditis elegans B0491.1 (B0491.1) mRNA, complete cds. 11/11 None
SNAP00000040222 ENSEMBL cdna:SNAP chromosome:CEL140:II:11348398:11349852:1 11/11 None
GENEFINDER00000040225 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11348398:11349852:1 11/11 None
B0491.1 ENSEMBL cdna:known chromosome:CEL140:II:11348398:11349852:1 gene:B0491.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11348394-11349849(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0491.1
Entrez Gene 174736 Entrez gene
SwissProt Q17515 EMBL-EBI
WormBase CE02103 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.18440.1.S1_AT similar to phosphatidylinositol glycan, class M cfa
DROSOPHILA_2:1625692_S_AT dm
DROSGENOME1:151873_AT dm
CHICKEN:GGAAFFX.6802.1.S1_S_AT phosphatidylinositol glycan, class M gga
HG-U133_PLUS_2:223470_AT phosphatidylinositol glycan, class M hs
HG-U133B:223470_AT phosphatidylinositol glycan, class M hs
HU35KSUBC:RC_W02102_AT phosphatidylinositol glycan, class M hs
U133_X3P:G11414878_3P_AT phosphatidylinositol glycan, class M hs
HG-U95C:64706_AT phosphatidylinositol glycan, class M hs
MG-U74CV2:168557_I_AT phosphatidylinositol glycan, class M mm
MG-U74CV2:166301_R_AT phosphatidylinositol glycan, class M mm
MG-U74CV2:170822_R_AT phosphatidylinositol glycan, class M mm
MOE430A:1418964_AT phosphatidylinositol glycan, class M mm
MOUSE430A_2:1418964_AT phosphatidylinositol glycan, class M mm
MOUSE430_2:1418964_AT phosphatidylinositol glycan, class M mm
MOE430A:1419877_X_AT Phosphatidylinositol glycan, class M (Pigm), mRNA mm
MOUSE430_2:1419877_X_AT Phosphatidylinositol glycan, class M (Pigm), mRNA mm
MOUSE430A_2:1419877_X_AT Phosphatidylinositol glycan, class M (Pigm), mRNA mm
RAE230A:1369042_AT phosphatidylinositol glycan, class M rn
RAT230_2:1369042_AT phosphatidylinositol glycan, class M rn
RAE230B:1397572_AT Phosphatidylinositol glycan, class M rn
RAT230_2:1397572_AT Phosphatidylinositol glycan, class M rn
RG-U34C:RC_AI236461_AT Phosphatidylinositol glycan, class M rn
YEAST_2:1772952_AT Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Sc
Protein Similarities
Method ID Description E-Value
blast CAA90090 Hypothetical protein B0491.1 [Caenorhabditis elegans] ref|NP_496426.1| B0491.1 [Caenorhabditis elegans] 0.0
blast CAE59524 Hypothetical protein CBG02919 [Caenorhabditis briggsae] 0.0
blast XP_785435 PREDICTED: similar to PIG-M mannosyltransferase [Strongylocentrotus purpuratus] 6.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR007704 EMBL-EBI Mannosyltransferase, DXD 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496426.1 8 13-35,85-107,145-167,182-204,224-246,282-304,353-375,379-401
B0491.1 8 13-35,85-107,145-167,182-204,224-246,282-304,353-375,379-401
SNAP00000040222 8 13-35,85-107,145-167,182-204,224-246,282-304,353-375,379-401
GENEFINDER00000040225 8 13-35,85-107,145-167,182-204,224-246,282-304,353-375,379-401
Sequence
Target Sequence 
>C. ELEGANS:179343_AT
atgctggtaactctcacatcattcgcagcggtggtactattcttcttccaaatctacgga
caactattcctcgatgaatatctgatctaccacgtcaaaagaagagatctcgcccataac
ttctcgccgtacttctacctattgtacctgtatgaagcgaatccgacaatgtcacagatc
atcggcctcggagcattcattccacaaattgtgctgatcgtgttcttcgccttcaagcat
tacgatgatcttcccttctgctggtttatcaccaccttcgcatttgttacatataacaaa
gtgtgcacaagtcagtactttgtctggtacattgttcttctacctttactcgctcataaa
attatgatgtcccgacaactagcgttatctctaatggccgcgtggttcgcaacacaagga
atctggcttctcgctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTGGTAACTCTCACATCATTCG 69 41 691 Antisense
GGTACTATTCTTCTTCCAAATCTAC 280 503 723 Antisense
GAAGAGATCTCGCCCATAACTTCTC 366 339 791 Antisense
TCGCCGTACTTCTACCTATTGTACC 161 599 814 Antisense
ACAATGTCACAGATCATCGGCCTCG 594 113 856 Antisense
GCCTCGGAGCATTCATTCCACAAAT 700 281 875 Antisense
AAATTGTGCTGATCGTGTTCTTCGC 598 115 896 Antisense
TGTTCTTCGCCTTCAAGCATTACGA 124 563 911 Antisense
GTACATTGTTCTTCTACCTTTACTC 38 461 1017 Antisense
TGATGTCCCGACAACTAGCGTTATC 636 565 1055 Antisense
ACACAAGGAATCTGGCTTCTCGCTG 266 111 1102 Antisense
Sequence Source Affymetrix Proprietary Database