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[179305_at]

Description ZK455.5 /REP_DB=WormBase Gene ID /WP=CE03816 /TR=Q23499 /GB=CAA91490.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 36      6grp_total > 07      4grp_total > 13      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 71.7
(1.4)
P 		85
(3)
P 		34.7
(-3)
A 		74.5
(1.7)
P 		50
B 55.9
(3)
P 		38.4
(-3)
A 		51.1
(1.4)
A 		42.9
(-1.5)
P 		18
C 66.4
(-0.9)
P 		32.4
(-3)
A 		53
(-1.7)
P 		130.9
(3)
A 		99
avr 64.7
(0) 		51.9
(-2.1) 		46.3
(-3) 		82.8
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 88.3
(3)
P 		59.2
(0.9)
P 		41.7
(-0.3)
A 		4.1
(-3)
A 		25.7
(-1.5)
A 		11.5
(-2.5)
A 		84
B 124.5
(3)
P 		55.8
(-0.4)
M 		72.5
(0.4)
P 		57.7
(-0.3)
A 		29.6
(-1.7)
P 		4.5
(-3)
A 		120
C 81.3
(3)
P 		33.2
(-1.8)
A 		37.9
(-1.3)
M 		42.2
(-0.9)
A 		33.4
(-1.8)
A 		21.1
(-3)
A 		60
avr 98
(3) 		49.4
(-0.4) 		50.7
(-0.3) 		34.7
(-1.4) 		29.6
(-1.8) 		12.4
(-3) 		86
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 26.1
(3)
P 		18.5
(0.3)
P 		9.1
(-3)
A 		10.9
(-2.4)
P 		17
B 17.8
(3)
P 		9.5
(-2.7)
A 		11.9
(-1.1)
A 		9.1
(-3)
P 		9
C 22
(3)
P 		9.3
(-3)
A 		18.5
(1.3)
P 		22.1
(3)
A 		13
avr 22
(3) 		12.4
(-3) 		13.2
(-2.5) 		14
(-2) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 16.3
(1.6)
P 		21.1
(3)
P 		9.1
(-0.6)
A 		1
(-3)
A 		7.8
(-1)
A 		2.8
(-2.5)
A 		20
B 43.3
(3)
P 		18.6
(-0.6)
M 		17.6
(-0.7)
P 		15.3
(-1)
A 		12
(-1.5)
P 		1.7
(-3)
A 		42
C 30.1
(3)
P 		10.1
(-2.2)
A 		9.6
(-2.3)
M 		8.5
(-2.6)
A 		10.8
(-2)
A 		7.1
(-3)
A 		23
avr 29.9
(3) 		16.6
(-0.1) 		12.1
(-1.1) 		8.3
(-2) 		10.2
(-1.5) 		3.9
(-3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179305_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16861
Sequence Type Exemplar sequence
Representative Public ID ZK455.5 NCBI
Target Description ZK455.5 /REP_DB=WormBase Gene ID /WP=CE03816 /TR=Q23499 /GB=CAA91490.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077494(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077494 NCBI Caenorhabditis elegans ZK455.5 (ZK455.5) mRNA, complete cds. 11/11 None
GENEFINDER00000004982 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11321079:11326674:-1 11/11 None
SNAP00000004974 ENSEMBL cdna:SNAP chromosome:CEL140:X:11325365:11326590:-1 11/11 None
ZK455.5 ENSEMBL cdna:known chromosome:CEL140:X:11325365:11326825:-1 gene:ZK455.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11325364-11326850(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK455.5
Entrez Gene 191321 Entrez gene
SwissProt Q23499 EMBL-EBI
WormBase CE37463 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91493 Hypothetical protein ZK455.4 [Caenorhabditis elegans] ref|NP_509894.2| Acid SphingoMyelinase family member (asm-2) [Caenorhabditis elegans] sp|Q23498|ASM2_CAEEL Sphingomyelin phosphodiesterase 2 precursor (Acid sphingomyelinase 2) 0.0
blast CAE70658 Hypothetical protein CBG17365 [Caenorhabditis briggsae] 0.0
blast CAA91490 Hypothetical protein ZK455.5 [Caenorhabditis elegans] ref|NP_509895.2| ZK455.5 [Caenorhabditis elegans] 1.0E-161
blast CAA91490 Hypothetical protein ZK455.5 [Caenorhabditis elegans] ref|NP_509895.2| ZK455.5 [Caenorhabditis elegans] 1.0E-154
blast CAA91490 Hypothetical protein ZK455.5 [Caenorhabditis elegans] ref|NP_509895.2| ZK455.5 [Caenorhabditis elegans] 1.0E-151
blast CAE70657 Hypothetical protein CBG17364 [Caenorhabditis briggsae] 4.0E-97
blast CAE70657 Hypothetical protein CBG17364 [Caenorhabditis briggsae] 5.0E-97
blast CAE73925 Hypothetical protein CBG21540 [Caenorhabditis briggsae] 7.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.5E-12
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.5E-126
Pfam IPR007856 EMBL-EBI Saposin-like type B, 1 1.6E-11
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509895.2 1 12-34
ZK455.5 1 12-34
GENEFINDER00000004982 1 12-34
Sequence
Target Sequence 
>C. ELEGANS:179305_AT
ggactcattcaactacggtgacgggtactatcagctcttctatcttttccgtgcaaacct
ggctcgtgctattcttcattataataaaccgttttggatgattcaacaagatacattttg
gaatgacaatctgttacacatcgatcttatggaaaacgaagacgtggacattctttttga
cagggcttctgacaaaggtcctctgattgcaggaggatattatcatgctaagccaacatc
aagctctaaaaattactttaacaaattagcaaaggacatttcctggtggtatgctcctga
taacgcctacatgacttcgctttgcgaagtttccggattggcaaaatgtggcagattatc
ctttgacctgataactaactggcaatggttgcaagggacttctggagttccacct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACTCATTCAACTACGGTGACGGG 615 523 264 Antisense
TGACGGGTACTATCAGCTCTTCTAT 342 569 282 Antisense
GGCTCGTGCTATTCTTCATTATAAT 585 539 324 Antisense
GACAATCTGTTACACATCGATCTTA 293 365 388 Antisense
GGACATTCTTTTTGACAGGGCTTCT 217 523 429 Antisense
CAGGGCTTCTGACAAAGGTCCTCTG 352 205 444 Antisense
AGGACATTTCCTGGTGGTATGCTCC 407 55 536 Antisense
GATAACGCCTACATGACTTCGCTTT 435 421 562 Antisense
TTCGCTTTGCGAAGTTTCCGGATTG 684 693 579 Antisense
GTGGCAGATTATCCTTTGACCTGAT 308 491 611 Antisense
GCAAGGGACTTCTGGAGTTCCACCT 354 319 654 Antisense
Sequence Source Affymetrix Proprietary Database