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[179300_at]

Description T28A8.6 /REP_DB=WormBase Gene ID /WP=CE18977 /TR=Q9XU04 /GB=CAB07289.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 96      4grp_high_8_8 > 07      6grp_total > 86      4grp_total > 16      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 37.7
(3)
P 		37.6
(2.9)
A 		35.3
(-0.1)
P 		33
(-3)
A 		5
B 35.9
(-2.5)
A 		34.2
(-3)
M 		55.3
(3)
P 		40.7
(-1.2)
P 		21
C 25.3
(-3)
A 		34.8
(-2)
P 		29.5
(-2.6)
P 		85.1
(3)
P 		60
avr 33
(-3) 		35.5
(-2.2) 		40
(-0.9) 		52.9
(3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 48.7
(-2.1)
P 		29.2
(-3)
A 		56.8
(-1.7)
P 		92
(0)
P 		111.1
(0.9)
P 		153.7
(3)
P 		125
B 32.1
(-3)
P 		47.6
(-2.5)
P 		65.8
(-1.9)
P 		73.3
(-1.6)
P 		122
(0.1)
P 		208.6
(3)
P 		177
C 27.3
(-3)
A 		53.6
(-2.1)
P 		62.6
(-1.8)
P 		106.1
(-0.4)
P 		93
(-0.9)
P 		210.7
(3)
P 		183
avr 36
(-3) 		43.5
(-2.7) 		61.7
(-2) 		90.5
(-0.9) 		108.7
(-0.2) 		191
(3) 		155
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 13.7
(3)
P 		8.2
(-0.7)
A 		9.2
(-0)
P 		4.8
(-3)
A 		9
B 11.5
(1.2)
A 		8.5
(-3)
M 		12.8
(3)
P 		8.7
(-2.7)
P 		4
C 8.4
(-3)
A 		10
(-1.4)
P 		10.3
(-1.1)
P 		14.4
(3)
P 		6
avr 11.2
(3) 		8.9
(-3) 		10.8
(2) 		9.3
(-2) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 9
(-3)
P 		10.4
(-2.7)
A 		12.4
(-2.3)
P 		23.3
(0)
P 		33.5
(2.2)
P 		37.4
(3)
P 		28
B 11.2
(-3)
P 		15.8
(-2.6)
P 		16
(-2.6)
P 		19.4
(-2.3)
P 		49.6
(0.5)
P 		77.8
(3)
P 		67
C 10.1
(-3)
A 		16.4
(-2.4)
P 		15.9
(-2.4)
P 		21.3
(-1.9)
P 		30.1
(-1)
P 		70.5
(3)
P 		60
avr 10.1
(-3) 		14.2
(-2.5) 		14.8
(-2.5) 		21.3
(-1.7) 		37.7
(0.2) 		61.9
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179300_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7667
Sequence Type Exemplar sequence
Representative Public ID T28A8.6 NCBI
Target Description T28A8.6 /REP_DB=WormBase Gene ID /WP=CE18977 /TR=Q9XU04 /GB=CAB07289.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067391(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067391 NCBI Caenorhabditis elegans T28A8.6 (T28A8.6) mRNA, complete cds. 11/11 None
SNAP00000022262 ENSEMBL cdna:SNAP chromosome:CEL140:III:13496504:13500903:1 11/11 None
GENEFINDER00000022270 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13498741:13500903:1 11/11 None
T28A8.6 ENSEMBL cdna:known chromosome:CEL140:III:13498720:13501009:1 gene:T28A8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13498736-13500899(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28A8.6
Entrez Gene 176780 Entrez gene
SwissProt Q9XU04 EMBL-EBI
WormBase CE18977 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07289 Hypothetical protein T28A8.6 [Caenorhabditis elegans] ref|NP_499792.1| T28A8.6 [Caenorhabditis elegans] 0.0
blast CAB55009 Hypothetical protein Y57A10A.4 [Caenorhabditis elegans] ref|NP_496584.1| Y57A10A.4 [Caenorhabditis elegans] 1.0E-136
blast CAB07287 Hypothetical protein T28A8.3 [Caenorhabditis elegans] ref|NP_499795.1| T28A8.3 [Caenorhabditis elegans] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR006570 EMBL-EBI SPK 1.7E-61
Pfam IPR006570 EMBL-EBI SPK 1.3E-66
Pfam IPR006570 EMBL-EBI SPK 1.7E-61
Pfam IPR006570 EMBL-EBI SPK 1.3E-66
Sequence
Target Sequence 
>C. ELEGANS:179300_AT
gacagccaaagcctgaaccaattgattttgatttttatcaaatcaataatcgagagcatt
tccaaggaaatcaagaaatgatgatagaagacaacaattcgtcaatttctgagagaaacg
ctgatgttccattcgtcgaacatcaacaaatcagtctttccgcggaaagtcgagatactt
cagctgaatcggaatccatatatctgcatgatttcctgaaacaactggctcaattcatta
gctttttcgaatgttcggagctagctgagatcaagaaaaatatcaaagaagtgatggctg
atgaggaaaaaattgccggctttcaaaaagtaaaaatccttgatattcgtacagttctcg
gtgcatttttcttcggagtcagccgaaaaatcaaatcaactggctcaaataattctacaa
tgaaggtcaaagatttcatgttcaagttcaagttcttccttctcggattggattgttccg
aacttctcgagctacagcagaaggttcatgagaaacttgatgagcaagatgttacaaaga
agattctcttgtacagcgacattcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAGCCAAAGCCTGAACCAATTGA 658 365 1292 Antisense
GACAACAATTCGTCAATTTCTGAGA 370 365 1381 Antisense
GAAACGCTGATGTTCCATTCGTCGA 126 359 1406 Antisense
GCGGAAAGTCGAGATACTTCAGCTG 634 293 1453 Antisense
GAATCGGAATCCATATATCTGCATG 321 329 1477 Antisense
GGCTCAATTCATTAGCTTTTTCGAA 266 539 1518 Antisense
GGTGCATTTTTCTTCGGAGTCAGCC 151 501 1651 Antisense
CAACTGGCTCAAATAATTCTACAAT 432 187 1688 Antisense
TGTTCCGAACTTCTCGAGCTACAGC 285 563 1765 Antisense
TCGAGCTACAGCAGAAGGTTCATGA 192 603 1778 Antisense
GATTCTCTTGTACAGCGACATTCAG 559 431 1833 Antisense
Sequence Source Affymetrix Proprietary Database