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[179295_s_at]

Description T04F8.1 /REP_DB=WormBase Gene ID /WP=CE03614 /TR=Q22161 /GB=CAA91477.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 04      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 61      6grp_total > 15      4grp_total > 55      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 571
1 2 3 4 range
A 1145
(3)
P 		912.2
(-2.8)
P 		904.3
(-3)
P 		902.7
(-3)
P 		242
B 1240.3
(3)
P 		884.8
(-0.7)
P 		990
(0.4)
P 		669
(-3)
P 		571
C 1037.2
(3)
P 		787.3
(-2.9)
P 		829.7
(-1.9)
P 		782
(-3)
P 		255
avr 1140.8
(3) 		861.4
(-1.7) 		908
(-0.9) 		784.6
(-3) 		356

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 365
1 2 3 4 5 6 range
A 616.1
(3)
P 		614.8
(3)
P 		407.6
(-1.3)
P 		381.3
(-1.9)
P 		408.1
(-1.3)
P 		327.1
(-3)
P 		289
B 479.1
(1.1)
P 		563.8
(3)
P 		387.5
(-1)
P 		388.5
(-1)
P 		322.4
(-2.5)
P 		300.1
(-3)
P 		264
C 575.9
(3)
P 		445.7
(0.6)
P 		489.7
(1.4)
P 		276.6
(-2.5)
P 		371.7
(-0.8)
P 		251.4
(-3)
P 		325
avr 557
(3) 		541.4
(2.6) 		428.3
(0.1) 		348.8
(-1.7) 		367.4
(-1.3) 		292.9
(-3) 		264
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 285
1 2 3 4 range
A 416.9
(3)
P 		198
(-1.6)
P 		236.4
(-0.8)
P 		132.1
(-3)
P 		285
B 395.5
(3)
P 		218.5
(-1.2)
P 		229.4
(-0.9)
P 		142.6
(-3)
P 		253
C 343.4
(3)
P 		226.3
(-0.3)
P 		289.2
(1.5)
P 		131.9
(-3)
P 		212
avr 385.3
(3) 		214.3
(-1.1) 		251.7
(-0.2) 		135.5
(-3) 		250

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 113.6
(-1.5)
P 		218.6
(3)
P 		88.8
(-2.6)
P 		96.7
(-2.3)
P 		123.2
(-1.1)
P 		79.6
(-3)
P 		139
B 166.7
(1.7)
P 		187.7
(3)
P 		94.1
(-3)
P 		102.9
(-2.4)
P 		131
(-0.6)
P 		111.9
(-1.9)
P 		94
C 213.5
(3)
P 		136
(0.1)
P 		124.6
(-0.4)
P 		55.6
(-3)
P 		120.3
(-0.5)
P 		84.2
(-1.9)
P 		158
avr 164.6
(2) 		180.8
(3) 		102.5
(-1.9) 		85.1
(-3) 		124.8
(-0.5) 		91.9
(-2.6) 		96
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179295_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16800
Sequence Type Exemplar sequence
Representative Public ID T04F8.1 NCBI
Target Description T04F8.1 /REP_DB=WormBase Gene ID /WP=CE03614 /TR=Q22161 /GB=CAA91477.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077548(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077548 NCBI Caenorhabditis elegans T04F8.1 (T04F8.1) mRNA, complete cds. 11/11 None
SNAP00000020401 ENSEMBL cdna:SNAP chromosome:CEL140:X:11659652:11661578:-1 11/11 None
GENEFINDER00000020411 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11659652:11661578:-1 11/11 None
T04F8.1 ENSEMBL cdna:known chromosome:CEL140:X:11659552:11661582:-1 gene:T04F8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11659651-11661578(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T04F8.1
Entrez Gene 181353 Entrez gene
SwissProt Q22161 EMBL-EBI
WormBase CE03614 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.25565.1.S1_AT similar to Sideroflexin-1 (Tricarboxylate carrier protein) (TCC) cfa
DROSOPHILA_2:1639257_S_AT dm
DROSGENOME1:149275_AT dm
HG-U133_PLUS_2:218392_X_AT sideroflexin 1 hs
HG-U133A_2:218392_X_AT sideroflexin 1 hs
HG-U133A:218392_X_AT sideroflexin 1 hs
HU35KSUBA:RC_AA599107_AT sideroflexin 1 hs
HU35KSUBB:RC_R00037_AT sideroflexin 1 hs
HU35KSUBB:RC_H65650_AT sideroflexin 1 hs
HG-U133_PLUS_2:232055_AT sideroflexin 1 hs
HG-U133B:232055_AT sideroflexin 1 hs
U133_X3P:G12232420_3P_X_AT sideroflexin 1 hs
U133_X3P:G12232420_3P_AT sideroflexin 1 hs
U133_X3P:HS.21255.0.A1_3P_AT sideroflexin 1 hs
U133_X3P:HS.16131.1.S1_3P_AT sideroflexin 1 hs
U133_X3P:218392_3P_X_AT sideroflexin 1 hs
HG-U133_PLUS_2:230069_AT sideroflexin 1 hs
HG-U133B:230069_AT sideroflexin 1 hs
HG-U95C:56878_AT sideroflexin 1 hs
HG-U95C:60033_AT sideroflexin 1 hs
HG-U95C:63475_AT sideroflexin 1 hs
HG-U95E:71019_AT sideroflexin 1 hs
HG-U95E:77672_AT sideroflexin 1 hs
HG-U95E:89278_R_AT sideroflexin 1 hs
HG-U133_PLUS_2:241223_X_AT Sideroflexin 1 hs
HG-U133B:241223_X_AT Sideroflexin 1 hs
HG-U95E:67564_AT Sideroflexin 1 hs
HG-U95D:69374_AT Sideroflexin 1 hs
HG-U95D:78925_AT Sideroflexin 1 hs
HG-U95D:86258_R_AT Sideroflexin 1 hs
HG-U95E:89420_R_AT Sideroflexin 1 hs
MG-U74AV2:92831_AT sideroflexin 1 mm
MG-U74BV2:112478_AT sideroflexin 1 mm
MOE430A:1417559_AT sideroflexin 1 mm
MOUSE430A_2:1417559_AT sideroflexin 1 mm
MOUSE430_2:1417559_AT sideroflexin 1 mm
MOE430A:1417560_AT sideroflexin 1 mm
MOUSE430A_2:1417560_AT sideroflexin 1 mm
MOUSE430_2:1417560_AT sideroflexin 1 mm
MU19KSUBC:TC36985_AT Sideroflexin 1 (Sfxn1), mRNA mm
MU19KSUBC:TC37849_S_AT Sideroflexin 1 (Sfxn1), mRNA mm
RG-U34B:RC_AA943004_S_AT sideroflexin 1 (predicted) rn
RAE230B:1397740_AT sideroflexin 1 (predicted) rn
RAT230_2:1397740_AT sideroflexin 1 (predicted) rn
RG-U34A:S70011_AT Sideroflexin 1 (predicted) rn
RG-U34A:S70011_G_AT Sideroflexin 1 (predicted) rn
RG-U34C:RC_AA819349_S_AT Sideroflexin 1 (predicted) rn
RAE230A:1372715_AT Sideroflexin 1 (predicted) rn
RAT230_2:1372715_AT Sideroflexin 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8324 cation transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91477 Hypothetical protein T04F8.1 [Caenorhabditis elegans] ref|NP_509949.1| T04F8.1 [Caenorhabditis elegans] 1.0E-170
blast CAE70599 Hypothetical protein CBG17273 [Caenorhabditis briggsae] 1.0E-164
blast NP_001016244 sideroflexin 3 [Xenopus tropicalis] 3.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR004686 EMBL-EBI Tricarboxylate/iron carrier 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509949.1 5 100-122,149-171,176-198,231-253,266-288
T04F8.1 5 100-122,149-171,176-198,231-253,266-288
SNAP00000020401 5 100-122,149-171,176-198,231-253,266-288
GENEFINDER00000020411 5 100-122,149-171,176-198,231-253,266-288
Sequence
Target Sequence 
>C. ELEGANS:179295_S_AT
caccaatggctgtcatcttctggcaatggttgaatcagtcgttcaacgcggttgtcaact
acacaaaccggtctggtgacggtggatccgtcagtcagcttctcgtatcgtactgcgcgg
ccactggaggtgctctcactgctgctcttggattgaactctcttgtcaagaaagcaccac
cacttgtaggacgtcttgtgccgttcgtggctgtttgtgtggccaattccatcaacattc
caatgatgagaagaggagagcttactgagggaatcgacattcttgacgagaacggacaag
tcattggccagtccccaggtgttgctcagtcagcaattagccaggtcgtagtttctagaa
ttttcatggcagtcccttcatttgctttcattccagtcgttgtgaatgccctcgagaagc
ggccatacttcaaggccaacccaaagatgttccttcctcttcaaaccattctttgcggtc
ttgttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACCAATGGCTGTCATCTTCTGGCA 56 203 359 Antisense
GAATCAGTCGTTCAACGCGGTTGTC 21 321 390 Antisense
CAGTCAGCTTCTCGTATCGTACTGC 467 207 450 Antisense
CACTGCTGCTCTTGGATTGAACTCT 40 199 495 Antisense
CTTGTAGGACGTCTTGTGCCGTTCG 53 217 541 Antisense
GGCTGTTTGTGTGGCCAATTCCATC 168 541 567 Antisense
ACGGACAAGTCATTGGCCAGTCCCC 13 93 650 Antisense
TAGAATTTTCATGGCAGTCCCTTCA 489 649 714 Antisense
TTCCAGTCGTTGTGAATGCCCTCGA 406 693 749 Antisense
AATGCCCTCGAGAAGCGGCCATACT 262 167 763 Antisense
AAACCATTCTTTGCGGTCTTGTTCT 683 125 821 Antisense
Sequence Source Affymetrix Proprietary Database