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[179294_at]

Description F43D2.2 /REP_DB=WormBase Gene ID /WP=CE10336 /TR=O17870 /GB=CAB05725.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 35      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 69
(-3)
A 		125.3
(3)
A 		82.8
(-1.5)
A 		109.9
(1.4)
A 		56
B 92.5
(-0.5)
A 		121.4
(3)
A 		78.1
(-2.2)
A 		71.9
(-3)
A 		50
C 84.8
(0.8)
A 		116.4
(2.6)
A 		20
(-3)
A 		122.7
(3)
A 		103
avr 82.1
(-0.8) 		121
(3) 		60.3
(-3) 		101.5
(1.1) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 105.5
(0.4)
A 		31.5
(-2.5)
A 		71.4
(-0.9)
A 		19.1
(-3)
A 		116.1
(0.9)
M 		170
(3)
P 		151
B 58.2
(-1.9)
A 		47.4
(-3)
A 		49.1
(-2.8)
A 		99.1
(2.4)
A 		86.2
(1.1)
A 		104.4
(3)
P 		57
C 55.3
(-0.6)
A 		51.2
(-0.8)
A 		9.2
(-3)
A 		35.6
(-1.6)
A 		99.9
(1.8)
P 		123.4
(3)
P 		114
avr 73
(-1) 		43.4
(-3) 		43.2
(-3) 		51.3
(-2.5) 		100.7
(0.9) 		132.6
(3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 25.1
(1.9)
A 		27.2
(3)
A 		21.6
(-0)
A 		16.1
(-3)
A 		11
B 29.5
(2.8)
A 		30
(3)
A 		18.1
(-1.9)
A 		15.3
(-3)
A 		15
C 28.1
(1.8)
A 		33.5
(3)
A 		7
(-3)
A 		20.7
(0.1)
A 		27
avr 27.6
(1.9) 		30.2
(3) 		15.6
(-3) 		17.4
(-2.3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 19.4
(-0.6)
A 		11.2
(-2)
A 		15.6
(-1.2)
A 		4.8
(-3)
A 		35
(2)
M 		41.4
(3)
P 		37
B 20.3
(-1.1)
A 		15.8
(-2.1)
A 		11.9
(-3)
A 		26.3
(0.2)
A 		35
(2.1)
A 		38.9
(3)
P 		27
C 20.5
(-0.2)
A 		15.6
(-1)
A 		2.4
(-3)
A 		7.2
(-2.3)
A 		32.3
(1.6)
P 		41.3
(3)
P 		39
avr 20.1
(-1) 		14.2
(-2.2) 		10
(-3) 		12.8
(-2.4) 		34.1
(1.7) 		40.5
(3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179294_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13309
Sequence Type Exemplar sequence
Representative Public ID F43D2.2 NCBI
Target Description F43D2.2 /REP_DB=WormBase Gene ID /WP=CE10336 /TR=O17870 /GB=CAB05725.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074216(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074216 NCBI Caenorhabditis elegans F43D2.2 (F43D2.2) mRNA, complete cds. 11/11 None
GENEFINDER00000010921 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14637944:14640340:-1 11/11 None
F43D2.2 ENSEMBL cdna:known chromosome:CEL140:V:14638171:14639751:-1 gene:F43D2.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000010914 4/11 Cross Hyb Matching Probes None
SNAP00000010915 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14638170-14639751(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F43D2.2
Entrez Gene 185705 Entrez gene
SwissProt O17870 EMBL-EBI
WormBase CE10336 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05725 Hypothetical protein F43D2.2 [Caenorhabditis elegans] ref|NP_506617.1| F43D2.2 [Caenorhabditis elegans] 1.0E-151
blast CAB05725 Hypothetical protein F43D2.2 [Caenorhabditis elegans] ref|NP_506617.1| F43D2.2 [Caenorhabditis elegans] 1.0E-144
blast CAB70252 Hypothetical protein M199.2 [Caenorhabditis elegans] ref|NP_502824.1| M199.2 [Caenorhabditis elegans] 6.0E-83
blast CAB70252 Hypothetical protein M199.2 [Caenorhabditis elegans] ref|NP_502824.1| M199.2 [Caenorhabditis elegans] 3.0E-78
blast CAB54365 Hypothetical protein Y105C5B.19 [Caenorhabditis elegans] ref|NP_502908.1| Y105C5B.19 [Caenorhabditis elegans] 8.0E-70
blast CAB54365 Hypothetical protein Y105C5B.19 [Caenorhabditis elegans] ref|NP_502908.1| Y105C5B.19 [Caenorhabditis elegans] 7.0E-64
Sequence
Target Sequence 
>C. ELEGANS:179294_AT
aatggcttttacttcagctatgtcaaacaagctgcacaagcaagttggtgagaaaactga
cgatcggaaacccacgggctcgaatgatgtcacatttttgatcgaatccgcagggccatg
ggacagagaaattgatgactcggatcctcacatgatgtgctacacgacatccaaaaatgc
atccacgtacatcccaattgacaaggatttcttcaatggaattagcgactgtctaatcgc
tggatttggtcaggagcactcaaaagacagaaaacttcaagtgagaaagctgatcactga
agttatgagctacaaactaagccgcgtgagagcccattacaacagcatcaatgggaatac
ttcgtcggagaaagctgccaaatggatgagagaggagattgctaaggaggatggggagct
aaaagaatgccagctcactctctcacacgcttcgaaaacttcacactggctcgaagattt
gccccaaaaagataaaaaactaatagattcttcaatcaaatgtctccagctgcaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGGCTTTTACTTCAGCTATGTCA 592 161 204 Antisense
GAAACCCACGGGCTCGAATGATGTC 308 357 270 Antisense
ATCGAATCCGCAGGGCCATGGGACA 243 35 304 Antisense
TGACTCGGATCCTCACATGATGTGC 345 569 339 Antisense
ATCCACGTACATCCCAATTGACAAG 286 41 384 Antisense
AAACTAAGCCGCGTGAGAGCCCATT 93 127 517 Antisense
GAGAGCCCATTACAACAGCATCAAT 42 395 531 Antisense
TAAAAGAATGCCAGCTCACTCTCTC 705 639 623 Antisense
CTCTCTCACACGCTTCGAAAACTTC 458 229 641 Antisense
CACACTGGCTCGAAGATTTGCCCCA 393 195 665 Antisense
CAATCAAATGTCTCCAGCTGCAATG 395 179 716 Antisense
Sequence Source Affymetrix Proprietary Database