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[179281_at]

Description F53H4.4 /REP_DB=WormBase Gene ID /WP=CE17863 /TR=Q93780 /GB=CAB03137.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 23      4grp_high_8_8 > 21      6grp_total > 02      4grp_total > 09      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 84
(-0.8)
P 		17.8
(-3)
A 		44.3
(-2.1)
A 		200.2
(3)
P 		182
B 60.2
(-3)
P 		82
(-1.2)
A 		86.5
(-0.8)
P 		132.8
(3)
P 		73
C 57.1
(-3)
A 		113.3
(2.1)
P 		92.9
(0.2)
P 		123.7
(3)
P 		67
avr 67.1
(-3) 		71
(-2.7) 		74.6
(-2.5) 		152.2
(3) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 90.1
(1.2)
P 		119.3
(3)
P 		24.2
(-3)
A 		83.7
(0.8)
A 		61.8
(-0.6)
M 		59.5
(-0.8)
P 		95
B 33.2
(-3)
A 		107.5
(3)
P 		46.5
(-1.9)
A 		62.8
(-0.6)
A 		74.3
(0.3)
P 		46.1
(-2)
A 		74
C 85.8
(0.8)
P 		45.3
(-3)
A 		88.8
(1.1)
P 		109.1
(3)
P 		71.5
(-0.5)
P 		62.2
(-1.4)
P 		64
avr 69.7
(-0.4) 		90.7
(3) 		53.2
(-3) 		85.2
(2.1) 		69.2
(-0.4) 		55.9
(-2.6) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 30.6
(3)
P 		3.9
(-3)
A 		11.6
(-1.3)
A 		29.3
(2.7)
P 		27
B 19.2
(-3)
P 		20.3
(-2.3)
A 		20
(-2.5)
P 		28.3
(3)
P 		9
C 18.9
(-3)
A 		32.6
(3)
P 		32.4
(2.9)
P 		20.9
(-2.1)
P 		14
avr 22.9
(0.3) 		18.9
(-3) 		21.3
(-1) 		26.2
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 16.6
(-1.2)
P 		42.4
(3)
P 		5.3
(-3)
A 		21.2
(-0.4)
A 		18.7
(-0.8)
M 		14.5
(-1.5)
P 		37
B 11.5
(-3)
A 		35.8
(3)
P 		11.3
(-3)
A 		16.6
(-1.7)
A 		30.2
(1.6)
P 		17.2
(-1.6)
A 		25
C 31.8
(3)
P 		13.8
(-3)
A 		22.6
(-0.1)
P 		21.9
(-0.3)
P 		23.2
(0.1)
P 		20.8
(-0.7)
P 		18
avr 20
(-0.6) 		30.7
(3) 		13.1
(-3) 		19.9
(-0.7) 		24
(0.7) 		17.5
(-1.5) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179281_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17369
Sequence Type Exemplar sequence
Representative Public ID F53H4.4 NCBI
Target Description F53H4.4 /REP_DB=WormBase Gene ID /WP=CE17863 /TR=Q93780 /GB=CAB03137.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078208(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078208 NCBI Caenorhabditis elegans F53H4.4 (F53H4.4) mRNA, complete cds. 11/11 None
SNAP00000026503 ENSEMBL cdna:SNAP chromosome:CEL140:X:15861552:15871131:1 9/11 None
GENEFINDER00000026509 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:15866529:15868505:1 9/11 None
F53H4.4 ENSEMBL cdna:known chromosome:CEL140:X:15866519:15868614:1 gene:F53H4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:15866522-15868227(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53H4.4
Entrez Gene 181675 Entrez gene
SwissProt Q93780 EMBL-EBI
WormBase CE17863 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03137 Hypothetical protein F53H4.4 [Caenorhabditis elegans] ref|NP_510609.1| F53H4.4 [Caenorhabditis elegans] 1.0E-158
blast CAB03137 Hypothetical protein F53H4.4 [Caenorhabditis elegans] ref|NP_510609.1| F53H4.4 [Caenorhabditis elegans] 1.0E-144
blast CAB03137 Hypothetical protein F53H4.4 [Caenorhabditis elegans] ref|NP_510609.1| F53H4.4 [Caenorhabditis elegans] 1.0E-132
blast CAE69729 Hypothetical protein CBG16000 [Caenorhabditis briggsae] 1.0E-106
blast CAE69729 Hypothetical protein CBG16000 [Caenorhabditis briggsae] 1.0E-103
blast CAE69729 Hypothetical protein CBG16000 [Caenorhabditis briggsae] 1.0E-95
blast CAE69731 Hypothetical protein CBG16002 [Caenorhabditis briggsae] 1.0E-61
blast CAE69731 Hypothetical protein CBG16002 [Caenorhabditis briggsae] 2.0E-61
Sequence
Target Sequence 
>C. ELEGANS:179281_AT
aatggtgtgcccggaacttatcagaattattgtggaaaatgcggataacaaatcgcgacg
agtaaaatttctaatcaaaaaagatgaatttgcaatatgcgttggacgcggccgacttat
tacatttccatttaccaaagacatggaagaacccgcatgtatacttgcagagtacaccga
attgctcttctcctttccacattatgatatcgagtattcatttgaaggtagctacggact
cagccggaatatggacctcacagaaacccacatcatgcttctcagcaaactaacacccag
tatgtttgctgaaaaggcgcgtccaaggagggtccgtgaaagaaatggtcgactcgctgt
ccgaatgagcagaatctcttggaatcgaagtggtctctactacaacgccataattcagtt
ttttgaatcactccgcggaactgtaatcacacaaggcgaacaaccattatgtgagaagtc
tgtgacttatcaggaaatgttgaagcacacgttggctcaccccggggtacatcagttgga
cagtcggcccaactttcagtggaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGGTGTGCCCGGAACTTATCAGA 453 163 243 Antisense
CGGCCGACTTATTACATTTCCATTT 404 235 351 Antisense
AGAGTACACCGAATTGCTCTTCTCC 228 69 411 Antisense
GACTCAGCCGGAATATGGACCTCAC 542 373 479 Antisense
CACAGAAACCCACATCATGCTTCTC 92 195 501 Antisense
TGCTTCTCAGCAAACTAACACCCAG 303 579 518 Antisense
TCGACTCGCTGTCCGAATGAGCAGA 70 603 591 Antisense
GAAGTGGTCTCTACTACAACGCCAT 560 335 629 Antisense
TCACTCCGCGGAACTGTAATCACAC 412 627 670 Antisense
GGTACATCAGTTGGACAGTCGGCCC 607 503 768 Antisense
AGTCGGCCCAACTTTCAGTGGAAGA 66 61 784 Antisense
Sequence Source Affymetrix Proprietary Database