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[179278_s_at]

Description F15D4.3 /REP_DB=WormBase Gene ID /WP=CE23658 /TR=Q93511 /GB=CAB02486.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 92      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 65      6grp_total > 84      4grp_total > 48      6grp_highest_sub > 04_05      6grp_high1_sub > 04_05      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 848
1 2 3 4 range
A 1220
(-3)
P 		1722
(1.3)
P 		1927.8
(3)
P 		1406
(-1.4)
P 		708
B 1266.6
(-2.5)
P 		1479.1
(-1)
P 		2038.8
(3)
P 		1190.4
(-3)
P 		848
C 1305
(-3)
P 		1515.4
(0.5)
P 		1670.4
(3)
P 		1640.7
(2.5)
P 		365
avr 1263.9
(-3) 		1572.2
(0) 		1879
(3) 		1412.4
(-1.6) 		615

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 729
1 2 3 4 5 6 range
A 905.8
(-3)
P 		1164.9
(-0.8)
P 		1344.4
(0.8)
P 		1601.2
(3)
P 		1115.9
(-1.2)
P 		1488.5
(2)
P 		695
B 871.9
(-3)
P 		1163.4
(-0.6)
P 		989.1
(-2)
P 		1055.1
(-1.5)
P 		1590.4
(3)
P 		1089.5
(-1.2)
P 		719
C 1284
(0.3)
P 		1135.3
(-2.6)
P 		1329
(1.1)
P 		1340.2
(1.3)
P 		1426.4
(3)
P 		1115.4
(-3)
P 		311
avr 1020.6
(-3) 		1154.5
(-0.7) 		1220.8
(0.4) 		1332.2
(2.2) 		1377.6
(3) 		1231.1
(0.5) 		357
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 376
1 2 3 4 range
A 444.2
(1.8)
P 		373.8
(0.4)
P 		504
(3)
P 		205.8
(-3)
P 		298
B 403.9
(1.1)
P 		365.3
(0.1)
P 		472.4
(3)
P 		253.7
(-3)
P 		219
C 432
(0.1)
P 		435.7
(0.1)
P 		582.2
(3)
P 		276.6
(-3)
P 		306
avr 426.7
(1) 		391.6
(0.2) 		519.5
(3) 		245.4
(-3) 		274

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 479
1 2 3 4 5 6 range
A 167
(-3)
P 		414.3
(3)
P 		292.8
(0.1)
P 		406.1
(2.8)
P 		336.7
(1.1)
P 		362.4
(1.7)
P 		247
B 303.4
(-2.1)
P 		387.2
(-0.8)
P 		240.2
(-3)
P 		279.5
(-2.4)
P 		646.1
(3)
P 		406.3
(-0.5)
P 		406
C 475.9
(3)
P 		346.4
(-0.8)
P 		338.3
(-1)
P 		269.5
(-3)
P 		461.6
(2.6)
P 		373.4
(0)
P 		206
avr 315.4
(-2.2) 		382.6
(-0.1) 		290.4
(-3) 		318.4
(-2.1) 		481.5
(3) 		380.7
(-0.2) 		191
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179278_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3196
Sequence Type Exemplar sequence
Representative Public ID F15D4.3 NCBI
Target Description F15D4.3 /REP_DB=WormBase Gene ID /WP=CE23658 /TR=Q93511 /GB=CAB02486.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064403(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064403 NCBI Caenorhabditis elegans F15D4.3 (F15D4.3) mRNA, complete cds. 11/11 A 
SNAP00000038515 ENSEMBL cdna:SNAP chromosome:CEL140:II:13237423:13242889:-1 10/11 None
GENEFINDER00000038527 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13237423:13237802:-1 11/11 None
F15D4.3 ENSEMBL cdna:known chromosome:CEL140:II:13237133:13237802:-1 gene:F15D4.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000042690 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13237414-13237799(-) 57.31 57.31
Public Domain and Genome References
Gene Title
Ensembl F15D4.3
Entrez Gene 174968 Entrez gene
SwissProt Q93511 EMBL-EBI
WormBase CE37223 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02487 Hypothetical protein F15D4.4 [Caenorhabditis elegans] ref|NP_496805.2| F15D4.4 [Caenorhabditis elegans] 0.0
blast CAE59447 Hypothetical protein CBG02822 [Caenorhabditis briggsae] 1.0E-166
blast BAA14402.1 unnamed protein product [Oryza sativa (japonica cultivar-group)] sp|P25776|ORYA_ORYSA Oryzain alpha chain precursor 6.0E-31
blast CAB02486 Hypothetical protein F15D4.3 [Caenorhabditis elegans] ref|NP_496804.2| F15D4.3 [Caenorhabditis elegans] 2.0E-21
blast CAE59446 Hypothetical protein CBG02821 [Caenorhabditis briggsae] 7.0E-17
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496804.2 1 24-46
F15D4.3 1 24-46
SNAP00000038515 1 486-508
GENEFINDER00000038527 1 24-46
Sequence
Target Sequence 
>C. ELEGANS:179278_S_AT
cgtcgtgttttacaaagattcgaatgggattaatgatgggagcaatgattggcggagcaa
caggcattcttctcggcggattcatgggcttcagagctggcatgcgaggcaaggatcttc
tcctacaaaccggcaaaactgtggcacaatccggcggatcattcggcgtcttcatgggtg
ttgcccagggtcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTCGTGTTTTACAAAGATTCGAAT 78 1 59 Antisense
GATGGGAGCAATGATTGGCGGAGCA 116 409 93 Antisense
TGGCGGAGCAACAGGCATTCTTCTC 147 545 108 Antisense
CTCGGCGGATTCATGGGCTTCAGAG 70 233 130 Antisense
GGCTTCAGAGCTGGCATGCGAGGCA 553 537 145 Antisense
GCATGCGAGGCAAGGATCTTCTCCT 18 311 158 Antisense
GATCTTCTCCTACAAACCGGCAAAA 426 417 172 Antisense
AACCGGCAAAACTGTGGCACAATCC 44 147 186 Antisense
GGCACAATCCGGCGGATCATTCGGC 616 527 201 Antisense
ATTCGGCGTCTTCATGGGTGTTGCC 306 7 219 Antisense
TTCATGGGTGTTGCCCAGGGTCTTC 475 683 229 Antisense
Sequence Source Affymetrix Proprietary Database