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[179255_at]

Description F28F8.4 /REP_DB=WormBase Gene ID /WP=CE09758 /TR=O17849 /GB=CAB03015.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 89      4grp_total > 22      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 32.1
(-0.9)
A 		38.4
(-0.3)
A 		13
(-3)
A 		68.8
(3)
A 		56
B 51.2
(2.6)
A 		39.1
(-0.4)
A 		28.4
(-3)
A 		52.7
(3)
A 		24
C 108.7
(3)
A 		9.8
(-2.7)
A 		4
(-3)
A 		69.4
(0.7)
P 		105
avr 64
(3) 		29.1
(-1.3) 		15.1
(-3) 		63.6
(3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 58.8
(2.5)
A 		54.3
(1.8)
A 		33
(-1.2)
A 		20.2
(-3)
A 		58.8
(2.5)
A 		62.4
(3)
A 		42
B 23.4
(-3)
A 		37.2
(-1.7)
A 		49.9
(-0.5)
A 		51.8
(-0.3)
A 		87.1
(3)
A 		46.5
(-0.8)
P 		64
C 66.1
(2.4)
A 		53
(1.2)
A 		44.9
(0.5)
A 		7.2
(-3)
A 		40.7
(0.1)
A 		72.7
(3)
A 		66
avr 49.4
(0.9) 		48.2
(0.7) 		42.6
(-0.3) 		26.4
(-3) 		62.2
(3) 		60.5
(2.7) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 11.7
(3)
A 		8.3
(0.5)
A 		3.4
(-3)
A 		10.1
(1.8)
A 		8
B 16.3
(3)
A 		9.7
(-1.1)
A 		6.6
(-3)
A 		11.2
(-0.2)
A 		10
C 36
(3)
A 		2.8
(-2.8)
A 		1.4
(-3)
A 		11.7
(-1.2)
P 		35
avr 21.3
(3) 		6.9
(-1.9) 		3.8
(-3) 		11
(-0.5) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 10.8
(-0.6)
A 		19.3
(3)
A 		7.2
(-2.1)
A 		5.1
(-3)
A 		17.7
(2.3)
A 		15.2
(1.3)
A 		14
B 8.2
(-3)
A 		12.4
(-2.1)
A 		12.1
(-2.1)
A 		13.7
(-1.8)
A 		35.4
(3)
A 		17.3
(-1)
P 		27
C 24.5
(3)
A 		16.2
(0.8)
A 		11.4
(-0.4)
A 		1.4
(-3)
A 		13.2
(0.1)
A 		24.3
(2.9)
A 		23
avr 14.5
(0) 		16
(0.6) 		10.2
(-1.6) 		6.7
(-3) 		22.1
(3) 		18.9
(1.8) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179255_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14005
Sequence Type Exemplar sequence
Representative Public ID F28F8.4 NCBI
Target Description F28F8.4 /REP_DB=WormBase Gene ID /WP=CE09758 /TR=O17849 /GB=CAB03015.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028698(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028698 NCBI Caenorhabditis elegans F28F8.8 (F28F8.8) mRNA, complete cds. 10/11 None
SNAP00000036075 ENSEMBL cdna:SNAP chromosome:CEL140:V:15575693:15576690:1 10/11 None
GENEFINDER00000036088 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15575693:15576690:1 10/11 None
F28F8.8 ENSEMBL cdna:known chromosome:CEL140:V:15574074:15576690:1 gene:F28F8.8 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15574072-15576690(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28F8.8
Entrez Gene 3564999 Entrez gene
SwissProt Q7YTT2 EMBL-EBI
WormBase CE34545 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE11303 Hypothetical protein F28F8.8 [Caenorhabditis elegans] ref|NP_001023869.1| F28F8.8 [Caenorhabditis elegans] 1.0E-180
blast CAE11303 Hypothetical protein F28F8.8 [Caenorhabditis elegans] ref|NP_001023869.1| F28F8.8 [Caenorhabditis elegans] 1.0E-173
blast CAB03015 Hypothetical protein F28F8.4 [Caenorhabditis elegans] ref|NP_506871.2| F28F8.4 [Caenorhabditis elegans] 1.0E-49
blast CAB03015 Hypothetical protein F28F8.4 [Caenorhabditis elegans] ref|NP_506871.2| F28F8.4 [Caenorhabditis elegans] 2.0E-45
blast CAB54328 Hypothetical protein Y113G7B.6 [Caenorhabditis elegans] ref|NP_507898.1| Y113G7B.6 [Caenorhabditis elegans] 4.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 9.9E-32
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.3E-13
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 9.9E-32
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 6.3E-11
Sequence
Target Sequence 
>C. ELEGANS:179255_AT
tgggcccggatttcgagggagtcgatcgaaagaaaacttcagagatcatccgaattatgt
tgaaatccgtgaaaaaagtggacgttggaaagctcacaatcactggattcatccatgagg
agatcccaatgattcttcaatactttggaagtgataatattcaagatctgaagatcgacg
atcacatttgcttcgaccatattggaaaagtcgtcgagtcgaatcactggaagaaggcaa
aatccattcatttcatctggaaaagctgtctttgcccagttttcatgcatctcacacact
ttagtgatattcagatccacatgaaaaatgtttccgtgcatgatgcaaaccgtatcattc
aggtaatctgtgctcggaacagcaatttacctacttattttaccaattttcagcttctcc
aaatctctgccaacttcaaatcgcttaacatcacaacatgttgcgtacaggattttcttc
ttgtcgttaaagttctccaaccaaatttcatccaaagagtttttggcagtggctctaccg
actttgccatcaacggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGGCCCGGATTTCGAGGGAGTCGA 292 549 1178 Antisense
GCTCACAATCACTGGATTCATCCAT 700 301 1269 Antisense
GATCACATTTGCTTCGACCATATTG 687 421 1357 Antisense
GGAAAAGCTGTCTTTGCCCAGTTTT 167 529 1436 Antisense
TTTTCATGCATCTCACACACTTTAG 47 677 1457 Antisense
GGAACAGCAATTTACCTACTTATTT 365 529 1553 Antisense
CTCTGCCAACTTCAAATCGCTTAAC 159 231 1602 Antisense
GCGTACAGGATTTTCTTCTTGTCGT 44 291 1640 Antisense
GTTCTCCAACCAAATTTCATCCAAA 143 443 1669 Antisense
AGAGTTTTTGGCAGTGGCTCTACCG 135 55 1693 Antisense
TCTACCGACTTTGCCATCAACGGCA 237 615 1711 Antisense
Sequence Source Affymetrix Proprietary Database