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[179254_at]

Description F25H8.6 /REP_DB=WormBase Gene ID /WP=CE05731 /TR=Q19787 /GB=CAA93282.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 09      6grp_high1_10_10 > 90      6grp_highest_nonorm > 72      6grp_total > 82      4grp_total > 57      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 480
1 2 3 4 range
A 111
(-3)
A 		413.1
(0.9)
P 		577.9
(3)
P 		109.4
(-3)
A 		469
B 97.8
(-3)
A 		546
(3)
P 		530.4
(2.8)
P 		137.1
(-2.5)
M 		448
C 122.7
(-3)
A 		500.3
(2.1)
P 		566.5
(3)
P 		169.8
(-2.4)
P 		444
avr 110.5
(-3) 		486.5
(2) 		558.3
(3) 		138.8
(-2.6) 		448

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 527
1 2 3 4 5 6 range
A 185.1
(-2.6)
P 		539.6
(2.7)
P 		217.9
(-2.1)
P 		461.3
(1.6)
P 		557.4
(3)
P 		157.6
(-3)
P 		400
B 202
(-1.3)
P 		424.7
(2.3)
P 		288.5
(0.1)
P 		464.9
(3)
P 		426.1
(2.4)
P 		97.7
(-3)
P 		367
C 214.2
(-2.5)
P 		453.6
(0.7)
P 		298.3
(-1.4)
P 		404.5
(0)
P 		625.1
(3)
P 		178.3
(-3)
P 		447
avr 200.4
(-2.1) 		472.6
(2) 		268.2
(-1.1) 		443.6
(1.6) 		536.2
(3) 		144.5
(-3) 		392
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 40.4
(-1.9)
A 		89.7
(0.3)
P 		151.1
(3)
P 		16
(-3)
A 		135
B 31.2
(-2.9)
A 		134.9
(3)
P 		122.9
(2.3)
P 		29.2
(-3)
M 		106
C 40.6
(-2.6)
A 		143.8
(1.1)
P 		197.5
(3)
P 		28.6
(-3)
P 		169
avr 37.4
(-2.4) 		122.8
(1.4) 		157.2
(3) 		24.6
(-3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 34.1
(-3)
P 		191.9
(3)
P 		47.4
(-2.5)
P 		117
(0.2)
P 		168.2
(2.1)
P 		38.4
(-2.8)
P 		158
B 70.3
(-1.5)
P 		141.4
(1.6)
P 		70.1
(-1.5)
P 		123.1
(0.8)
P 		173.1
(3)
P 		36.4
(-3)
P 		137
C 79.4
(-2.2)
P 		138.4
(0.3)
P 		75.9
(-2.3)
P 		81.4
(-2.1)
P 		202.3
(3)
P 		59.7
(-3)
P 		143
avr 61.3
(-2.3) 		157.2
(1.9) 		64.5
(-2.1) 		107.2
(-0.3) 		181.2
(3) 		44.8
(-3) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179254_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9166
Sequence Type Exemplar sequence
Representative Public ID F25H8.6 NCBI
Target Description F25H8.6 /REP_DB=WormBase Gene ID /WP=CE05731 /TR=Q19787 /GB=CAA93282.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069384(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069384 NCBI Caenorhabditis elegans F25H8.6 (F25H8.6) mRNA, complete cds. 11/11 None
GENEFINDER00000013895 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9916519:9921509:1 11/11 None
F25H8.6 ENSEMBL cdna:known chromosome:CEL140:IV:9916519:9921912:1 gene:F25H8.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013886 3/11 Cross Hyb Matching Probes None
SNAP00000013887 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9916524-9921515(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25H8.6
Entrez Gene 184940 Entrez gene
SwissProt Q19787 EMBL-EBI
WormBase CE05731 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93282 Hypothetical protein F25H8.6 [Caenorhabditis elegans] ref|NP_501785.1| F25H8.6 [Caenorhabditis elegans] 0.0
blast CAE59968 Hypothetical protein CBG03458 [Caenorhabditis briggsae] 0.0
blast CAE59968 Hypothetical protein CBG03458 [Caenorhabditis briggsae] 1.0E-174
Protein Domains
Database ID Description E-Value
Pfam IPR003656 EMBL-EBI BED finger 3.5E-18
Sequence
Target Sequence 
>C. ELEGANS:179254_AT
acatgcccagtggtgatgtcttccaagccacaatagttcctcaattactgaatcaaatga
aacaacaaatcgaagcccttgttcataatagcagctcattaagcagcattcctgatcaag
tgatgacttcatcatctgccacgtcatcatatgaagatgtttcagtaaacgaatctcaga
tggctggaccaaatgtgggtgatgaggaggaagaaattatggaagaagaagttgaggaag
atgagaatgttgagatcgaagacgatacatcgtcagcatcatcgtcaattgacacggata
cttgtgatgcaatggcttcattcattcattttattggaaatgacgcatttccccatgatg
agctcatttctcttctgtccgtggtcacaaatctcttcacctacttctcaactcgtccac
atgttcaaacacatcttcagatgactatcatccagccgacatctcagccattagttcaac
aagtccaatttgttacctcaaacctgtcaataatatctgactatatccgacagactcctg
atatgcagcttctcccacttgcagtaaatcagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATGCCCAGTGGTGATGTCTTCCA 423 107 1193 Antisense
CTTCCAAGCCACAATAGTTCCTCAA 48 219 1212 Antisense
GCCCTTGTTCATAATAGCAGCTCAT 529 285 1267 Antisense
GCAGCTCATTAAGCAGCATTCCTGA 470 313 1283 Antisense
GAAATGACGCATTTCCCCATGATGA 9 363 1529 Antisense
ATGATGAGCTCATTTCTCTTCTGTC 79 47 1547 Antisense
CGTGGTCACAAATCTCTTCACCTAC 384 245 1572 Antisense
TTCTCAACTCGTCCACATGTTCAAA 6 691 1597 Antisense
CCGACATCTCAGCCATTAGTTCAAC 365 263 1648 Antisense
CAGACTCCTGATATGCAGCTTCTCC 565 205 1723 Antisense
CTTCTCCCACTTGCAGTAAATCAGG 576 219 1741 Antisense
Sequence Source Affymetrix Proprietary Database