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[179252_at]

Description F53B2.6 /REP_DB=WormBase Gene ID /WP=CE17851 /TR=Q9XTH7 /GB=CAA98132.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 54      6grp_total > 54      4grp_total > 57      6grp_cgc > 45      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 107.7
(-2.8)
P 		215.6
(0.3)
P 		307.1
(3)
P 		101.8
(-3)
A 		205
B 120.8
(-1.7)
M 		276.7
(3)
P 		278.1
(3)
P 		78.8
(-3)
A 		199
C 93.1
(-1.4)
A 		315.9
(3)
P 		287.5
(2.4)
P 		14.6
(-3)
A 		301
avr 107.2
(-1.9) 		269.4
(2.4) 		290.9
(3) 		65.1
(-3) 		226

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 295
1 2 3 4 5 6 range
A 108.1
(-3)
M 		302.5
(3)
P 		264.8
(1.8)
P 		186.5
(-0.6)
P 		131.5
(-2.3)
P 		284.5
(2.4)
P 		194
B 168.6
(-1.1)
P 		223.9
(1.6)
P 		253.6
(3)
P 		221.6
(1.5)
P 		128.5
(-3)
P 		230.8
(1.9)
P 		125
C 276
(-0.3)
P 		242.1
(-1.2)
P 		402.9
(3)
P 		229.5
(-1.5)
P 		176.1
(-2.9)
P 		173.7
(-3)
P 		229
avr 184.2
(-1.6) 		256.2
(1.1) 		307.1
(3) 		212.5
(-0.5) 		145.4
(-3) 		229.7
(0.1) 		162
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 39.2
(-0.8)
P 		46.8
(-0.1)
P 		80.3
(3)
P 		14.9
(-3)
A 		65
B 38.5
(-0.5)
M 		68.3
(3)
P 		64.5
(2.6)
P 		16.8
(-3)
A 		52
C 30.8
(-1.3)
A 		90.8
(2.4)
P 		100.2
(3)
P 		2.5
(-3)
A 		98
avr 36.2
(-0.9) 		68.6
(1.9) 		81.7
(3) 		11.4
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 19.9
(-3)
M 		107.6
(3)
P 		57.7
(-0.4)
P 		47.3
(-1.1)
P 		39.7
(-1.6)
P 		69.3
(0.4)
P 		88
B 58.7
(-1.8)
P 		74.5
(1)
P 		61.6
(-1.3)
P 		58.7
(-1.8)
P 		52.2
(-3)
P 		86
(3)
P 		34
C 102.3
(3)
P 		73.9
(-0)
P 		102.6
(3)
P 		46.1
(-3)
P 		57
(-1.8)
P 		58.1
(-1.7)
P 		57
avr 60.3
(-1.2) 		85.3
(3) 		74
(1.1) 		50.7
(-2.8) 		49.6
(-3) 		71.1
(0.6) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179252_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8740
Sequence Type Exemplar sequence
Representative Public ID F53B2.6 NCBI
Target Description F53B2.6 /REP_DB=WormBase Gene ID /WP=CE17851 /TR=Q9XTH7 /GB=CAA98132.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070000(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070000 NCBI Caenorhabditis elegans HSN Abnormal Migration family member (ham-1) (ham-1) mRNA, complete cds. 11/11 None
F53B2.6 ENSEMBL cdna:known chromosome:CEL140:IV:12542005:12545685:1 gene:F53B2.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000026530 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12542192-12545307(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ham-1
Ensembl F53B2.6
Entrez Gene 178205 Entrez gene
SwissProt Q9XTH7 EMBL-EBI
WormBase CE17851 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98132 Hypothetical protein F53B2.6 [Caenorhabditis elegans] emb|CAB05844.1| Hypothetical protein F53B2.6 [Caenorhabditis elegans] ref|NP_502401.1| HSN Abnormal Migration family member (ham-1) [Caenorhabditis elegans] 0.0
blast CAE67925 Hypothetical protein CBG13523 [Caenorhabditis briggsae] 1.0E-171
blast XP_125685 PREDICTED: similar to winged helix domain-containing protein isoform A [Mus musculus] 9.0E-26
Sequence
Target Sequence 
>C. ELEGANS:179252_AT
agcatttttgcaaggctcttctctcgtggcatgaagcctcaaactattatgccatcagcg
tcaccgattcatgttggaggacctccaacaccaccaccagctgctatgttgcctaccaag
aataagtacccgacttatcatcacgatctcaacgaagagtgtcaacggaaggctcgaaga
cgtaaccacccccgtcgtggcgagacccaaaagctactctcgtctagttcggaatgtctc
aagtactatccagttgatatgccagagacccgaccgactcgtcgaagggcccgcatggct
tcaccactccgaagttccacaccgaacaactcggattcggcctactcgatctctccacca
cataccgattcaaatgaggaagctggcagtatcagtgattcggagatcaatcacacatat
atcaatatcaacaagttccgccgtcagaatttcgattccacgcagtttgaggatctgaca
ggtgccacagcgacaacttcagaggagcccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCATTTTTGCAAGGCTCTTCTCTC 145 79 700 Antisense
CTCTTCTCTCGTGGCATGAAGCCTC 669 229 715 Antisense
GAAGCCTCAAACTATTATGCCATCA 151 345 732 Antisense
TCAGCGTCACCGATTCATGTTGGAG 439 625 754 Antisense
AGCTGCTATGTTGCCTACCAAGAAT 467 81 798 Antisense
TACCCGACTTATCATCACGATCTCA 181 649 826 Antisense
CAAAAGCTACTCTCGTCTAGTTCGG 637 189 907 Antisense
CGAACAACTCGGATTCGGCCTACTC 205 251 1022 Antisense
CAAGTTCCGCCGTCAGAATTTCGAT 54 185 1131 Antisense
ATCTGACAGGTGCCACAGCGACAAC 114 33 1172 Antisense
ACAGCGACAACTTCAGAGGAGCCCG 370 109 1186 Antisense
Sequence Source Affymetrix Proprietary Database