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[179246_s_at]

Description F54B8.1 /REP_DB=WormBase Gene ID /WP=CE16103 /TR=O45558 /GB=CAB07622.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 84      4grp_total > 40      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 48.5
(-3)
A 		111.1
(-1.3)
P 		276
(3)
P 		82.3
(-2.1)
A 		228
B 59.9
(-3)
A 		153.4
(1.5)
P 		183.6
(3)
P 		136.7
(0.7)
A 		124
C 46.4
(-3)
A 		88.2
(-0.8)
A 		61.7
(-2.2)
A 		160
(3)
P 		114
avr 51.6
(-3) 		117.6
(0.2) 		173.8
(3) 		126.3
(0.7) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 181
1 2 3 4 5 6 range
A 12.9
(-3)
A 		85.6
(0.1)
A 		116.3
(1.3)
P 		104.8
(0.9)
P 		100.7
(0.7)
P 		155.7
(3)
P 		143
B 43
(-3)
A 		100.5
(-0.7)
P 		94.9
(-0.9)
P 		96.7
(-0.9)
P 		193.8
(3)
P 		131.9
(0.5)
P 		151
C 20.1
(-3)
A 		105.7
(1.1)
P 		103.9
(1)
P 		120.7
(1.9)
P 		144.3
(3)
P 		134.6
(2.5)
P 		124
avr 25.3
(-3) 		97.3
(0.6) 		105
(1) 		107.4
(1.1) 		146.3
(3) 		140.7
(2.7) 		121
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 17.7
(-2.4)
A 		24.1
(-1.8)
P 		72.2
(3)
P 		12.1
(-3)
A 		60
B 19.1
(-3)
A 		37.9
(1.8)
P 		42.6
(3)
P 		29.1
(-0.4)
A 		24
C 15.4
(-3)
A 		25.4
(2.2)
A 		21.5
(0.2)
A 		27
(3)
P 		12
avr 17.4
(-3) 		29.1
(-0.5) 		45.4
(3) 		22.7
(-1.9) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 2.4
(-3)
A 		30.5
(1.7)
A 		25.3
(0.9)
P 		26.6
(1.1)
P 		30.4
(1.7)
P 		37.9
(3)
P 		36
B 15
(-3)
A 		33.4
(-1.3)
P 		23
(-2.2)
P 		25.6
(-2)
P 		78.7
(3)
P 		49.2
(0.2)
P 		64
C 7.5
(-3)
A 		32.2
(0.8)
P 		26.4
(-0.1)
P 		24.3
(-0.4)
P 		46.7
(3)
P 		45.1
(2.8)
P 		39
avr 8.3
(-3) 		32
(0.3) 		24.9
(-0.7) 		25.5
(-0.6) 		51.9
(3) 		44.1
(1.9) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179246_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13377
Sequence Type Exemplar sequence
Representative Public ID F54B8.1 NCBI
Target Description F54B8.1 /REP_DB=WormBase Gene ID /WP=CE16103 /TR=O45558 /GB=CAB07622.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074377(11), NM_074545(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074377 NCBI Caenorhabditis elegans M01B2.8 (M01B2.8) mRNA, complete cds. 11/11 None
NM_074545 NCBI Caenorhabditis elegans F54B8.1 (F54B8.1) mRNA, complete cds. 11/11 None
SNAP00000027840 ENSEMBL cdna:SNAP chromosome:CEL140:V:15262824:15265094:1 11/11 None
GENEFINDER00000027850 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15262824:15263451:1 11/11 None
SNAP00000032674 ENSEMBL cdna:SNAP chromosome:CEL140:V:15813368:15813914:1 11/11 None
GENEFINDER00000032683 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15813368:15813914:1 11/11 None
M01B2.8 ENSEMBL cdna:known chromosome:CEL140:V:15262824:15263372:1 gene:M01B2.8 11/11 None
F54B8.1 ENSEMBL cdna:known chromosome:CEL140:V:15813368:15813914:1 gene:F54B8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15813367-15813914(+) 100.0 100.0
chrV:15262823-15263368(+) 94.48 94.48
Public Domain and Genome References
Gene Title

Ensembl M01B2.8
F54B8.1
Entrez Gene 186199 Entrez gene
187359 Entrez gene
SwissProt O45558 EMBL-EBI
O17962 EMBL-EBI
WormBase CE16103 Wormbase
CE16269 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07622 Hypothetical protein F54B8.1 [Caenorhabditis elegans] ref|NP_506946.1| F54B8.1 [Caenorhabditis elegans] 5.0E-79
blast CAB05566 Hypothetical protein M01B2.8 [Caenorhabditis elegans] ref|NP_506778.1| M01B2.8 [Caenorhabditis elegans] 9.0E-76
blast CAB05566 Hypothetical protein M01B2.8 [Caenorhabditis elegans] ref|NP_506778.1| M01B2.8 [Caenorhabditis elegans] 4.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 1.0E-26
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 8.6E-43
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 3.6E-39
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 4.8E-42
Sequence
Target Sequence 
>C. ELEGANS:179246_S_AT
atgttccagcagcttctcatatttttgagtttaattgaaggtttgcttgcttgtattccc
actcaacaaattgagccaccgactgaagcacctttcccctgcaatgtttgctcaaaaatt
tacaactctggctgccaaggatttggacttccaagtgcatcaaattggtgctctacagct
gctcaggttccagtcagttatactttaggcgtggggcctccggaggcttcctcattaccc
gacgtttgctcaagccaattcacatgtcctgctggaacttttattaaagttacattgatc
aatggggtcactgttatctccggaaatacgaatggtgcacctcaagttgtgtactgtttt
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTCCAGCAGCTTCTCATATTTT 317 47 13 Antisense
CTTGCTTGTATTCCCACTCAACAAA 216 219 58 Antisense
TCCCACTCAACAAATTGAGCCACCG 259 599 69 Antisense
CAAATTGGTGCTCTACAGCTGCTCA 621 193 173 Antisense
AGCTGCTCAGGTTCCAGTCAGTTAT 6 81 189 Antisense
GTCAGTTATACTTTAGGCGTGGGGC 626 463 205 Antisense
CCGACGTTTGCTCAAGCCAATTCAC 677 263 251 Antisense
CAAGCCAATTCACATGTCCTGCTGG 531 185 263 Antisense
TGTCCTGCTGGAACTTTTATTAAAG 234 557 277 Antisense
TGATCAATGGGGTCACTGTTATCTC 92 565 308 Antisense
GCACCTCAAGTTGTGTACTGTTTTG 147 297 349 Antisense
Sequence Source Affymetrix Proprietary Database