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[179227_at]

Description R10E4.6 /REP_DB=WormBase Gene ID /WP=CE03560 /TR=Q21903 /GB=CAA90767.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 77      4grp_total > 34      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 28.7
(-1.2)
A 		8.6
(-3)
A 		29.8
(-1.1)
A 		75.7
(3)
A 		67
B 11.2
(-1.8)
A 		27.7
(2.2)
A 		30.8
(3)
A 		6.3
(-3)
A 		25
C 7.3
(-2.8)
A 		52.7
(3)
A 		5.5
(-3)
A 		11.6
(-2.2)
A 		47
avr 15.7
(-3) 		29.7
(2.4) 		22
(-0.6) 		31.2
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 6.8
(-2.8)
A 		7.8
(-2.7)
A 		22.4
(-1)
A 		5
(-3)
A 		23.1
(-1)
A 		58
(3)
A 		53
B 10.9
(-1.6)
A 		36.4
(3)
A 		30.6
(1.9)
A 		3.3
(-3)
A 		14.5
(-1)
A 		28.9
(1.6)
A 		33
C 5.4
(-2.7)
A 		3.8
(-3)
A 		6.3
(-2.6)
A 		12.1
(-1.5)
A 		25.6
(0.9)
A 		37.4
(3)
A 		34
avr 7.7
(-2.8) 		16
(-1.4) 		19.8
(-0.7) 		6.8
(-3) 		21.1
(-0.5) 		41.4
(3) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 10.4
(2.5)
A 		1.9
(-3)
A 		7.8
(0.8)
A 		11.1
(3)
A 		9
B 3.6
(-0.6)
A 		6.8
(2.7)
A 		7.1
(3)
A 		1.3
(-3)
A 		6
C 2.4
(-2.8)
A 		15.2
(3)
A 		1.9
(-3)
A 		1.9
(-3)
A 		13
avr 5.5
(-1.7) 		8
(3) 		5.6
(-1.5) 		4.8
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 1.3
(-3)
A 		2.8
(-2.3)
A 		4.9
(-1.3)
A 		1.3
(-3)
A 		7
(-0.3)
A 		14.1
(3)
A 		13
B 3.8
(-1.4)
A 		12.1
(3)
A 		7.4
(0.5)
A 		0.9
(-3)
A 		5.9
(-0.3)
A 		10.8
(2.3)
A 		11
C 2
(-2.6)
A 		1.2
(-3)
A 		1.6
(-2.8)
A 		2.4
(-2.4)
A 		8.3
(0.8)
A 		12.5
(3)
A 		11
avr 2.4
(-2.5) 		5.4
(-0.9) 		4.6
(-1.3) 		1.5
(-3) 		7.1
(0.1) 		12.5
(3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179227_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7166
Sequence Type Exemplar sequence
Representative Public ID R10E4.6 NCBI
Target Description R10E4.6 /REP_DB=WormBase Gene ID /WP=CE03560 /TR=Q21903 /GB=CAA90767.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065459(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065459 NCBI Caenorhabditis elegans R10E4.6 (R10E4.6) mRNA, complete cds. 11/11 None
GENEFINDER00000016407 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4293429:4296477:1 11/11 None
R10E4.6 ENSEMBL cdna:known chromosome:CEL140:III:4293378:4294895:1 gene:R10E4.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000016399 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4293377-4294895(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R10E4.6
Entrez Gene 187771 Entrez gene
SwissProt Q21903 EMBL-EBI
WormBase CE03560 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90767 Hypothetical protein R10E4.6 [Caenorhabditis elegans] ref|NP_497860.1| R10E4.6 [Caenorhabditis elegans] 0.0
blast CAA90768 Hypothetical protein R10E4.7 [Caenorhabditis elegans] ref|NP_497861.1| R10E4.7 [Caenorhabditis elegans] 2.0E-93
blast CAE66918 Hypothetical protein CBG12306 [Caenorhabditis briggsae] 1.0E-64
blast CAE66917 Hypothetical protein CBG12305 [Caenorhabditis briggsae] 3.0E-50
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497860.1 1 21-43
R10E4.6 1 21-43
Sequence
Target Sequence 
>C. ELEGANS:179227_AT
agataccgattcctgttgttaatttaacaattcatattcctgatcgatggttcagagacc
ctggaagtaggtctccactgatgtacgtatgttatgaaggtgagacgtttctaaaaattc
acccaattgaaacaaaaccgatggattggggagaaaatgccagatttcggacaacttcta
tcgtaaattcatataatgagaggtctaggatcagagagattctagtggttctcgatgatt
tttggaacaatcgatatcttctaccacggaatctggatgctcgagcaatccttgacacaa
agtccatacttccaaggatcacatttggaaaggaagaacaaccatgttgtctatggaagt
tccaattcgaagacattgatggagtcccacaggcatttcatcgatgctttggaagtattc
catcagaagcactgaaatcaaatcagattcctgaagcattggcggatctattgctacgtg
gcaaaatgacaagacggtttctgtcgccaccaacgagagtttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATACCGATTCCTGTTGTTAATTT 624 65 530 Antisense
GATCGATGGTTCAGAGACCCTGGAA 311 417 571 Antisense
AGGTCTCCACTGATGTACGTATGTT 564 57 598 Antisense
GCCAGATTTCGGACAACTTCTATCG 146 281 688 Antisense
ATCGATATCTTCTACCACGGAATCT 564 35 779 Antisense
GGATGCTCGAGCAATCCTTGACACA 623 513 804 Antisense
AAGTCCATACTTCCAAGGATCACAT 320 157 829 Antisense
GATGGAGTCCCACAGGCATTTCATC 368 407 907 Antisense
TTGGCGGATCTATTGCTACGTGGCA 491 703 988 Antisense
TGACAAGACGGTTTCTGTCGCCACC 441 571 1016 Antisense
TCTGTCGCCACCAACGAGAGTTTGT 433 605 1029 Antisense
Sequence Source Affymetrix Proprietary Database