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[179219_at]

Description F25C8.3 /REP_DB=WormBase Gene ID /WP=CE18636 /TR=Q9XV66 /GB=CAB04172.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 15      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 20      6grp_total > 15      4grp_total > 47      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1439
1 2 3 4 range
A 1871.4
(3)
P 		747.7
(-2.1)
P 		547.2
(-3)
P 		651
(-2.5)
P 		1324
B 1666.9
(3)
P 		736
(-2.6)
P 		664.1
(-3)
P 		827.3
(-2)
P 		1003
C 1986.1
(3)
P 		601.7
(-3)
P 		610.6
(-3)
P 		701.1
(-2.6)
P 		1384
avr 1841.5
(3) 		695.1
(-2.6) 		607.3
(-3) 		726.5
(-2.4) 		1234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1107
1 2 3 4 5 6 range
A 1574.4
(2.6)
P 		1641.8
(3)
P 		546.4
(-3)
P 		754.1
(-1.9)
P 		702.4
(-2.1)
P 		701.5
(-2.2)
P 		1095
B 1493.4
(3)
P 		1301.4
(1.8)
P 		677.8
(-2.1)
P 		969.2
(-0.3)
P 		582.3
(-2.7)
P 		535.1
(-3)
P 		958
C 1427.4
(3)
P 		1132.9
(1)
P 		1130.6
(1)
P 		791.8
(-1.3)
P 		778.9
(-1.4)
P 		550.7
(-3)
P 		877
avr 1498.4
(3) 		1358.7
(2.1) 		784.9
(-1.7) 		838.4
(-1.4) 		687.9
(-2.4) 		595.8
(-3) 		903
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 586
1 2 3 4 range
A 681.4
(3)
P 		162.3
(-2.3)
P 		143.1
(-2.5)
P 		95.3
(-3)
P 		586
B 531.6
(3)
P 		181.8
(-2.6)
P 		153.9
(-3)
P 		176.3
(-2.6)
P 		378
C 657.4
(3)
P 		173
(-2.4)
P 		212.8
(-1.9)
P 		118.2
(-3)
P 		539
avr 623.5
(3) 		172.4
(-2.5) 		169.9
(-2.5) 		129.9
(-3) 		494

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 465
1 2 3 4 5 6 range
A 290.2
(-0.8)
P 		583.9
(3)
P 		119
(-3)
P 		191.3
(-2.1)
P 		211.9
(-1.8)
P 		170.8
(-2.3)
P 		465
B 519.6
(3)
P 		433.2
(1.5)
P 		164.6
(-3)
P 		256.7
(-1.4)
P 		236.6
(-1.8)
P 		199.5
(-2.4)
P 		355
C 529.1
(3)
P 		345.7
(0)
P 		287.8
(-0.9)
P 		159.2
(-3)
P 		252
(-1.5)
P 		184.4
(-2.6)
P 		370
avr 446.3
(2.8) 		454.3
(3) 		190.5
(-2.9) 		202.4
(-2.6) 		233.5
(-1.9) 		184.9
(-3) 		269
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179219_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12704
Sequence Type Exemplar sequence
Representative Public ID F25C8.3 NCBI
Target Description F25C8.3 /REP_DB=WormBase Gene ID /WP=CE18636 /TR=Q9XV66 /GB=CAB04172.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028669(11), NM_001028668(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028669 NCBI Caenorhabditis elegans F25C8.3b (F25C8.3) mRNA, complete cds. 11/11 None
NM_001028668 NCBI Caenorhabditis elegans F25C8.3a (F25C8.3) mRNA, complete cds. 11/11 None
SNAP00000010149 ENSEMBL cdna:SNAP chromosome:CEL140:V:20888594:20896789:1 11/11 None
GENEFINDER00000010160 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20888353:20896789:1 11/11 None
F25C8.3a ENSEMBL cdna:known chromosome:CEL140:V:20877905:20896789:1 gene:F25C8.3 11/11 None
F25C8.3b ENSEMBL cdna:known chromosome:CEL140:V:20877905:20896948:1 gene:F25C8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20877904-20896789(+) 99.57 100.0
Public Domain and Genome References
Ensembl F25C8.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE46668 Hypothetical protein F25C8.3b [Caenorhabditis elegans] ref|NP_001023840.1| F25C8.3b [Caenorhabditis elegans] 0.0
blast CAB04172 Hypothetical protein F25C8.3a [Caenorhabditis elegans] ref|NP_001023839.1| F25C8.3a [Caenorhabditis elegans] 0.0
blast CAE61629 Hypothetical protein CBG05555 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:179219_AT
gttacacgccaacaatggccgacccacattcagattcatcagctgcttcgactgcccccc
gtggtgcaagttcccgtcaaagtattgatcgacgaacaagcgttcacatgaaaaaagttt
taccaactatgaaagaagatataccagaagatccagaagattcggaagatgttatagatt
ctaactcaacagggcaagttacttctagaataagcaaaagcccgagcatacctcttaaca
aaacacatcaatcgagtcggactcggtcagtcagtggatttggaatgtggagaagtgttc
gaaggaaatcacgtcatgtgtcaagtgctgaggaatcgtctgaagagcgcggcagcgtag
aactacacgatgttggccatcattctgctcttcacgaaccaaatcgtactccaaatcgtc
gatcgacagaagcattggttttaccgttacacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTACACGCCAACAATGGCCGACCC 327 433 8894 Antisense
CGACCCACATTCAGATTCATCAGCT 489 251 8913 Antisense
CAGATTCATCAGCTGCTTCGACTGC 201 207 8924 Antisense
GCAAAAGCCCGAGCATACCTCTTAA 181 319 9107 Antisense
AGTCGGACTCGGTCAGTCAGTGGAT 68 61 9148 Antisense
GAGGAATCGTCTGAAGAGCGCGGCA 479 403 9223 Antisense
GAGCGCGGCAGCGTAGAACTACACG 400 385 9238 Antisense
CACGATGTTGGCCATCATTCTGCTC 70 201 9259 Antisense
CATTCTGCTCTTCACGAACCAAATC 610 215 9274 Antisense
GAACCAAATCGTACTCCAAATCGTC 505 335 9289 Antisense
GAAGCATTGGTTTTACCGTTACACG 50 341 9322 Antisense
Sequence Source Affymetrix Proprietary Database