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[179218_at]

Description W02A2.8 /REP_DB=WormBase Gene ID /WP=CE21238 /TR=Q9XUB1 /GB=CAB05311.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 79      4grp_total > 27      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 7.5
(-2.8)
A 		32.3
(-1)
A 		4
(-3)
A 		89.5
(3)
P 		86
B 10.6
(-2.7)
A 		54.1
(3)
A 		8.4
(-3)
A 		24.9
(-0.8)
A 		46
C 58
(2.5)
A 		37.5
(-0.3)
A 		18.4
(-3)
A 		61.6
(3)
P 		43
avr 25.4
(-1.1) 		41.3
(0.8) 		10.3
(-3) 		58.7
(3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 26
(-1.6)
A 		51.4
(0.4)
A 		16.3
(-2.3)
A 		7.7
(-3)
A 		50.1
(0.3)
P 		85.4
(3)
A 		78
B 34.9
(-0.9)
A 		34.5
(-1)
A 		28.8
(-1.4)
A 		10.1
(-3)
A 		37.4
(-0.7)
P 		81.7
(3)
P 		72
C 2.4
(-3)
A 		30.9
(-0)
A 		14.9
(-1.7)
A 		49.1
(1.9)
A 		55.6
(2.5)
A 		60.1
(3)
P 		58
avr 21.1
(-2.9) 		38.9
(-1) 		20
(-3) 		22.3
(-2.8) 		47.7
(-0) 		75.7
(3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 2.7
(-2.2)
A 		7
(-0)
A 		1
(-3)
A 		13.1
(3)
P 		12
B 3.4
(-2.2)
A 		13.4
(3)
A 		1.9
(-3)
A 		5.3
(-1.2)
A 		12
C 19.2
(3)
A 		10.8
(-0.9)
A 		6.4
(-3)
A 		10.4
(-1.1)
P 		13
avr 8.4
(1.4) 		10.4
(3) 		3.1
(-3) 		9.6
(2.3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 4.8
(-2.1)
A 		18.3
(2.2)
A 		3.6
(-2.5)
A 		2
(-3)
A 		15.1
(1.2)
P 		20.8
(3)
A 		19
B 12.2
(-0.9)
A 		11.5
(-1.1)
A 		7
(-2.1)
A 		2.7
(-3)
A 		15.2
(-0.3)
P 		30.5
(3)
P 		28
C 0.9
(-3)
A 		9.4
(-0.3)
A 		3.8
(-2.1)
A 		9.9
(-0.2)
A 		18
(2.3)
A 		20.1
(3)
P 		19
avr 6
(-2.6) 		13.1
(-0.4) 		4.8
(-3) 		4.9
(-3) 		16.1
(0.6) 		23.8
(3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179218_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9003
Sequence Type Exemplar sequence
Representative Public ID W02A2.8 NCBI
Target Description W02A2.8 /REP_DB=WormBase Gene ID /WP=CE21238 /TR=Q9XUB1 /GB=CAB05311.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070166(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070166 NCBI Caenorhabditis elegans W02A2.8 (W02A2.8) mRNA, complete cds. 11/11 None
SNAP00000017640 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13356161:13357736:-1 9/11 None
GENEFINDER00000017646 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13356161:13357586:-1 11/11 None
W02A2.8 ENSEMBL cdna:known chromosome:CEL140:IV:13356161:13357391:-1 gene:W02A2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13356166-13357397(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W02A2.8
Entrez Gene 189099 Entrez gene
SwissProt Q9XUB1 EMBL-EBI
WormBase CE21238 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05311 Hypothetical protein W02A2.8 [Caenorhabditis elegans] ref|NP_502567.1| W02A2.8 [Caenorhabditis elegans] 5.0E-74
blast CAB05311 Hypothetical protein W02A2.8 [Caenorhabditis elegans] ref|NP_502567.1| W02A2.8 [Caenorhabditis elegans] 2.0E-73
blast CAE66690 Hypothetical protein CBG12030 [Caenorhabditis briggsae] 7.0E-63
blast CAB05311 Hypothetical protein W02A2.8 [Caenorhabditis elegans] ref|NP_502567.1| W02A2.8 [Caenorhabditis elegans] 3.0E-57
blast CAE66690 Hypothetical protein CBG12030 [Caenorhabditis briggsae] 2.0E-54
blast CAE66690 Hypothetical protein CBG12030 [Caenorhabditis briggsae] 1.0E-48
Sequence
Target Sequence 
>C. ELEGANS:179218_AT
ttgtggaagcgtcctcattcaacgtggaataagattactgcagtgacaaatgtgagctct
gaaaagacaataaccgatggacaattgagtgaaaaagctcgtaaagatgttgatcatgat
gtagatcgaacaatttatgatattccactcgaatattgtcatcttcgaattccaaaatca
cgtacatcaagatcttgtcccgatccgtggagacgatatggagctcatgttaaactttgt
gaggttggtggttggatgcctgaaaatcgaaacaagaattctaaagtgattgtagtgaag
aaaggatcagctccgccagcggctccccgttgtaaaccactgatgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTGGAAGCGTCCTCATTCAACGT 68 709 25 Antisense
TACTGCAGTGACAAATGTGAGCTCT 314 567 60 Antisense
ATTCCACTCGAATATTGTCATCTTC 420 7 166 Antisense
AATATTGTCATCTTCGAATTCCAAA 698 173 176 Antisense
GAATTCCAAAATCACGTACATCAAG 247 323 191 Antisense
GTACATCAAGATCTTGTCCCGATCC 663 459 206 Antisense
TCTTGTCCCGATCCGTGGAGACGAT 449 617 217 Antisense
ATGGAGCTCATGTTAAACTTTGTGA 506 51 242 Antisense
CTTTGTGAGGTTGGTGGTTGGATGC 632 221 259 Antisense
TGAAGAAAGGATCAGCTCCGCCAGC 192 575 320 Antisense
CTCCCCGTTGTAAACCACTGATGAC 237 233 347 Antisense
Sequence Source Affymetrix Proprietary Database