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[179214_at]

Description F54B8.4 /REP_DB=WormBase Gene ID /WP=CE16106 /TR=O45562 /GB=CAB07626.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 28      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 20      6grp_total > 18      4grp_total > 13      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3214
1 2 3 4 range
A 1580
(-0.6)
P 		314.7
(-2.9)
P 		275.2
(-3)
P 		3488.8
(3)
P 		3214
B 1698.2
(-0.1)
P 		315.6
(-3)
P 		364.8
(-2.9)
P 		3133.5
(3)
P 		2818
C 2051.1
(0.9)
P 		377.9
(-3)
P 		357.8
(-3)
P 		2981
(3)
P 		2623
avr 1776.4
(0) 		336.1
(-3) 		332.6
(-3) 		3201.1
(3) 		2869

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1143
1 2 3 4 5 6 range
A 1559
(3)
P 		891.5
(-1.5)
P 		797
(-2.2)
P 		1041.3
(-0.5)
P 		675
(-3)
P 		861.5
(-1.7)
P 		884
B 1791.1
(3)
P 		829.9
(-2.5)
P 		821.6
(-2.5)
P 		735.9
(-3)
P 		958
(-1.7)
P 		851
(-2.3)
P 		1055
C 1739.4
(3)
P 		745
(-2.5)
P 		647.7
(-3)
P 		916.1
(-1.5)
P 		902.5
(-1.6)
P 		1034.6
(-0.9)
P 		1092
avr 1696.5
(3) 		822.1
(-2.6) 		755.4
(-3) 		897.8
(-2.1) 		845.2
(-2.4) 		915.7
(-2) 		941
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 611
1 2 3 4 range
A 575.3
(3)
P 		68.3
(-3)
P 		71.9
(-3)
P 		510.7
(2.2)
P 		507
B 541.5
(1.7)
P 		77.9
(-3)
P 		84.5
(-2.9)
P 		667.7
(3)
P 		590
C 679
(3)
P 		108.6
(-3)
P 		124.7
(-2.8)
P 		502.6
(1.1)
P 		570
avr 598.6
(3) 		84.9
(-3) 		93.7
(-2.9) 		560.3
(2.6) 		514

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 480
1 2 3 4 5 6 range
A 287.4
(1.8)
P 		317.1
(3)
P 		173.6
(-3)
P 		264.1
(0.8)
P 		203.7
(-1.7)
P 		209.7
(-1.5)
P 		144
B 623.2
(3)
P 		276.2
(-1.9)
P 		199.5
(-2.9)
P 		194.9
(-3)
P 		389.2
(-0.3)
P 		317.3
(-1.3)
P 		428
C 644.7
(3)
P 		227.3
(-2.2)
P 		164.8
(-3)
P 		184.2
(-2.8)
P 		292
(-1.4)
P 		346.3
(-0.7)
P 		480
avr 518.4
(3) 		273.5
(-1.3) 		179.3
(-3) 		214.4
(-2.4) 		295
(-1) 		291.1
(-1) 		339
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179214_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13697
Sequence Type Exemplar sequence
Representative Public ID F54B8.4 NCBI
Target Description F54B8.4 /REP_DB=WormBase Gene ID /WP=CE16106 /TR=O45562 /GB=CAB07626.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074548(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074548 NCBI Caenorhabditis elegans F54B8.4 (F54B8.4) mRNA, complete cds. 11/11 None
SNAP00000032677 ENSEMBL cdna:SNAP chromosome:CEL140:V:15819280:15819645:1 11/11 None
GENEFINDER00000032682 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15819280:15819645:1 11/11 None
F54B8.4 ENSEMBL cdna:known chromosome:CEL140:V:15819280:15819645:1 gene:F54B8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15819279-15819645(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54B8.4
Entrez Gene 186201 Entrez gene
SwissProt O45562 EMBL-EBI
WormBase CE16106 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07626 Hypothetical protein F54B8.4 [Caenorhabditis elegans] ref|NP_506949.1| F54B8.4 [Caenorhabditis elegans] 2.0E-50
blast CAE66557 Hypothetical protein CBG11869 [Caenorhabditis briggsae] 2.0E-21
blast CAA96692 Hypothetical protein T28F4.5 [Caenorhabditis elegans] ref|NP_492102.1| T28F4.5 [Caenorhabditis elegans] 3.0E-21
Sequence
Target Sequence 
>C. ELEGANS:179214_AT
gataacaacgctgtggtcggtcaccaaccagcaacaaaagttggaggacgtcgtattgct
gaccatggaaaccaaagagctcaaaactctacaaactcctcagatgcagtccgtgaagct
atggattttgatttaccggcaaagatggaaaaagcctatccgactgccgcggtaaagcag
gtgcacgaaaaaccaactccggcaattcaaccagctcactataatcgtgcaaccggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATAACAACGCTGTGGTCGGTCACC 560 423 25 Antisense
GTCGGTCACCAACCAGCAACAAAAG 523 471 40 Antisense
GGAGGACGTCGTATTGCTGACCATG 254 515 67 Antisense
GCTCAAAACTCTACAAACTCCTCAG 475 301 103 Antisense
ACTCCTCAGATGCAGTCCGTGAAGC 131 99 119 Antisense
AGCTATGGATTTTGATTTACCGGCA 565 77 141 Antisense
TGGAAAAAGCCTATCCGACTGCCGC 624 547 170 Antisense
GACTGCCGCGGTAAAGCAGGTGCAC 543 373 186 Antisense
GGTGCACGAAAAACCAACTCCGGCA 336 499 204 Antisense
GCAATTCAACCAGCTCACTATAATC 323 323 226 Antisense
GCTCACTATAATCGTGCAACCGGCA 318 299 238 Antisense
Sequence Source Affymetrix Proprietary Database