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[179204_s_at]

Description T27F2.4 /REP_DB=WormBase Gene ID /WP=CE06522 /TR=Q22835 /GB=CAA98551.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 78      4grp_highest_8_8 > 22      4grp_high_8_8 > 15      6grp_highest_nonorm > 96      6grp_total > 96      4grp_total > 09      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1253
1 2 3 4 range
A 261.6
(-3)
P 		364.4
(-2.5)
P 		562.5
(-1.6)
P 		1514.9
(3)
P 		1253
B 272.9
(-3)
P 		316.8
(-2.8)
P 		407.4
(-2.3)
P 		1498
(3)
P 		1225
C 268.6
(-3)
P 		313.3
(-2.8)
P 		484.9
(-1.9)
P 		1433.4
(3)
P 		1165
avr 267.7
(-3) 		331.5
(-2.7) 		484.9
(-1.9) 		1482.1
(3) 		1214

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 909
1 2 3 4 5 6 range
A 851
(-0.8)
P 		799.6
(-1.2)
P 		562.7
(-3)
P 		1001.9
(0.3)
P 		919.4
(-0.3)
P 		1351.3
(3)
P 		789
B 762.6
(-0.2)
P 		700.1
(-0.7)
P 		442.1
(-3)
P 		839.2
(0.5)
P 		980.2
(1.7)
P 		1128
(3)
P 		686
C 901.4
(-0.7)
P 		800.1
(-2.4)
P 		767.9
(-3)
P 		938.1
(-0)
P 		970.8
(0.5)
P 		1113.1
(3)
P 		345
avr 838.3
(-0.6) 		766.6
(-1.3) 		590.9
(-3) 		926.4
(0.3) 		956.8
(0.6) 		1197.5
(3) 		607
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 241
1 2 3 4 range
A 95.2
(-2.3)
P 		79.1
(-3)
P 		147
(-0.1)
P 		221.7
(3)
P 		143
B 87
(-2.8)
P 		78.2
(-3)
P 		94.4
(-2.6)
P 		319.2
(3)
P 		241
C 88.9
(-3)
P 		90.1
(-3)
P 		169
(0.1)
P 		241.7
(3)
P 		153
avr 90.4
(-2.7) 		82.5
(-3) 		136.8
(-1.2) 		260.9
(3) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 313
1 2 3 4 5 6 range
A 156.9
(-2)
P 		284.4
(1.7)
P 		122.5
(-3)
P 		254.1
(0.8)
P 		277.4
(1.5)
P 		329
(3)
P 		207
B 265.4
(0)
P 		233
(-0.6)
P 		107.4
(-3)
P 		222.3
(-0.8)
P 		398.3
(2.6)
P 		420.6
(3)
P 		313
C 334.1
(1.7)
P 		244.1
(-1.2)
P 		195.4
(-2.8)
P 		188.7
(-3)
P 		314.1
(1.1)
P 		372.6
(3)
P 		184
avr 252.1
(-0.2) 		253.8
(-0.1) 		141.8
(-3) 		221.7
(-0.9) 		329.9
(1.9) 		374.1
(3) 		232
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179204_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12760
Sequence Type Exemplar sequence
Representative Public ID T27F2.4 NCBI
Target Description T27F2.4 /REP_DB=WormBase Gene ID /WP=CE06522 /TR=Q22835 /GB=CAA98551.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073550(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073550 NCBI Caenorhabditis elegans T27F2.4 (T27F2.4) mRNA, complete cds. 11/11 None
SNAP00000017030 ENSEMBL cdna:SNAP chromosome:CEL140:V:11652792:11653340:-1 11/11 None
GENEFINDER00000017032 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11652792:11653340:-1 11/11 None
T27F2.4 ENSEMBL cdna:known chromosome:CEL140:V:11652737:11653344:-1 gene:T27F2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11652791-11653340(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T27F2.4
Entrez Gene 179599 Entrez gene
SwissProt Q22835 EMBL-EBI
WormBase CE06522 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98551 Hypothetical protein T27F2.4 [Caenorhabditis elegans] ref|NP_505951.1| T27F2.4 [Caenorhabditis elegans] 4.0E-62
blast CAE75320 Hypothetical protein CBG23294 [Caenorhabditis briggsae] 1.0E-41
Sequence
Target Sequence 
>C. ELEGANS:179204_S_AT
tctaattcagtttcctctgttcctgaatctctattctcatcagcttcaattcatcgtcca
gttgctattaatccagcaatgcttgctcaattcagcatcaatctgcctgttttgccattt
gaaagttctgcttctttgggaacatctaccacatccagctcgagatgctcttcaactgaa
agttctgctgctcctggtaaaatccgtcgtggtcgaccacaacaagaaattgcagatgga
caagatgctcacagtcaaaagaagcgtcatcgccgtctttatgctcgccagtaccgtgct
caaatgcgccaaaaggtggagaatgtgaaaagtcttcatgatgagaaggagcaactcgag
ttggaagtaaaagctctccggcaagcagttagtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTAATTCAGTTTCCTCTGTTCCTG 577 611 40 Antisense
TGAATCTCTATTCTCATCAGCTTCA 2 577 63 Antisense
AGCTTCAATTCATCGTCCAGTTGCT 91 77 81 Antisense
TCAGCATCAATCTGCCTGTTTTGCC 675 623 131 Antisense
TTTGGGAACATCTACCACATCCAGC 324 667 174 Antisense
ATCCAGCTCGAGATGCTCTTCAACT 599 39 192 Antisense
CAACTGAAAGTTCTGCTGCTCCTGG 29 189 212 Antisense
GCTCCTGGTAAAATCCGTCGTGGTC 617 287 229 Antisense
TCAAAAGAAGCGTCATCGCCGTCTT 29 631 294 Antisense
TCGCCAGTACCGTGCTCAAATGCGC 685 597 324 Antisense
AAAGCTCTCCGGCAAGCAGTTAGTG 516 123 409 Antisense
Sequence Source Affymetrix Proprietary Database