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[179200_at]

Description T28C6.7 /REP_DB=WormBase Gene ID /WP=CE21216 /TR=Q22847 /GB=CAA90998.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 01      4grp_highest_8_8 > 42      4grp_high_8_8 > 40      6grp_highest_nonorm > 51      6grp_total > 14      4grp_total > 55      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 377
1 2 3 4 range
A 655.8
(3)
P 		375.6
(-3)
P 		459.7
(-1.2)
P 		385.7
(-2.8)
P 		280
B 668.3
(3)
P 		399.4
(-3)
P 		430.6
(-2.3)
P 		480.6
(-1.2)
P 		269
C 614.2
(3)
P 		472.6
(0.4)
P 		498.6
(0.9)
P 		291.5
(-3)
P 		323
avr 646.1
(3) 		415.9
(-2.3) 		463
(-1.2) 		385.9
(-3) 		260

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 727
1 2 3 4 5 6 range
A 685.1
(1.6)
P 		774.5
(3)
P 		380
(-3)
P 		588
(0.2)
P 		459.2
(-1.8)
P 		451.5
(-1.9)
P 		395
B 839.5
(3)
P 		540.8
(-0.9)
P 		608.8
(0)
P 		577.5
(-0.4)
P 		437.7
(-2.2)
P 		375.3
(-3)
P 		464
C 998.4
(3)
P 		698.1
(0.5)
P 		829.5
(1.6)
P 		538.4
(-0.8)
P 		503.4
(-1.1)
P 		271.7
(-3)
P 		727
avr 841
(3) 		671.1
(0.9) 		606.1
(0) 		568
(-0.4) 		466.8
(-1.7) 		366.2
(-3) 		475
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 238.8
(3)
P 		81.5
(-2.2)
P 		120.2
(-0.9)
P 		56.5
(-3)
P 		182
B 213.1
(3)
P 		98.7
(-3)
P 		99.8
(-2.9)
P 		102.4
(-2.8)
P 		114
C 203.3
(3)
P 		135.9
(0.4)
P 		173.8
(1.9)
P 		49.1
(-3)
P 		154
avr 218.4
(3) 		105.4
(-1.5) 		131.3
(-0.5) 		69.3
(-3) 		149

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 287
1 2 3 4 5 6 range
A 126.3
(-1.6)
P 		275.5
(3)
P 		82.8
(-3)
P 		149.1
(-0.9)
P 		138.6
(-1.3)
P 		109.9
(-2.2)
P 		193
B 292.1
(3)
P 		180
(-1.4)
P 		147.8
(-2.7)
P 		153
(-2.5)
P 		177.8
(-1.5)
P 		139.9
(-3)
P 		152
C 370.1
(3)
P 		213
(-0.4)
P 		211.1
(-0.4)
P 		108.3
(-2.6)
P 		162.9
(-1.5)
P 		90.9
(-3)
P 		279
avr 262.8
(3) 		222.8
(1.4) 		147.2
(-1.6) 		136.8
(-2.1) 		159.8
(-1.1) 		113.6
(-3) 		149
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179200_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9202
Sequence Type Exemplar sequence
Representative Public ID T28C6.7 NCBI
Target Description T28C6.7 /REP_DB=WormBase Gene ID /WP=CE21216 /TR=Q22847 /GB=CAA90998.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069127(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069127 NCBI Caenorhabditis elegans T28C6.7 (T28C6.7) mRNA, complete cds. 11/11 None
SNAP00000005658 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8831595:8839913:1 11/11 None
GENEFINDER00000005660 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8830633:8839913:1 11/11 None
T28C6.7 ENSEMBL cdna:known chromosome:CEL140:IV:8827786:8840638:1 gene:T28C6.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8827791-8840334(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28C6.7
Entrez Gene 177697 Entrez gene
SwissProt Q22847 EMBL-EBI
WormBase CE32942 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.12875.1.S1_AT similar to centrosomal protein 2 cfa
CHICKEN:GGA.7522.1.S1_AT similar to centrosomal protein 2; centrosome associated protein; centrosomal Nek2-associated protein 1 gga
CHICKEN:GGAAFFX.1161.2.S1_S_AT similar to centrosomal protein 2; centrosome associated protein; centrosomal Nek2-associated protein 1 gga
CHICKEN:GGAAFFX.1161.3.S1_S_AT similar to centrosomal protein 2; centrosome associated protein; centrosomal Nek2-associated protein 1 gga
HG-U133_PLUS_2:209495_AT centrosomal protein 2 hs
HG-U133A:209495_AT centrosomal protein 2 hs
HG-U133A_2:209495_AT centrosomal protein 2 hs
HG-U133_PLUS_2:210894_S_AT centrosomal protein 2 hs
HG-U133A:210894_S_AT centrosomal protein 2 hs
HG-FOCUS:210894_S_AT centrosomal protein 2 hs
HG-U133A_2:210894_S_AT centrosomal protein 2 hs
HG-U95AV2:35206_AT centrosomal protein 2 hs
HU35KSUBB:RC_R87989_AT centrosomal protein 2 hs
HU35KSUBC:RC_AA251153_AT centrosomal protein 2 hs
U133_X3P:G2832236_3P_AT centrosomal protein 2 hs
U133_X3P:G12655156_3P_S_AT centrosomal protein 2 hs
HG-U95C:56138_AT centrosomal protein 2 hs
HG-U95C:56139_AT centrosomal protein 2 hs
HG-U95E:74643_AT centrosomal protein 2 hs
MOUSE430_2:1421512_AT centrosomal protein 2 mm
MOUSE430A_2:1421512_AT centrosomal protein 2 mm
MOE430A:1421512_AT centrosomal protein 2 mm
MU11KSUBA:U33198_S_AT centrosomal protein 2 mm
MG-U74AV2:101145_AT centrosomal protein 2 mm
MG-U74CV2:135659_AT PREDICTED: centrosomal protein 2 [Mus musculus], mRNA sequence mm
MG-U74CV2:169516_AT PREDICTED: centrosomal protein 2 [Mus musculus], mRNA sequence mm
RAE230A:1377062_AT similar to centrosomal Nek2-associated protein 1 rn
RAT230_2:1377062_AT similar to centrosomal Nek2-associated protein 1 rn
Protein Similarities
Method ID Description E-Value
blast CAA90998 Hypothetical protein T28C6.7 [Caenorhabditis elegans] ref|NP_501528.2| T28C6.7 [Caenorhabditis elegans] 0.0
blast XP_653447 Viral A-type inclusion protein repeat, putative [Entamoeba histolytica HM-1:IMSS] gb|EAL48061.1| Viral A-type inclusion protein repeat, putative [Entamoeba histolytica HM-1:IMSS] 1.0E-43
blast CAE56384 Hypothetical protein CBG24066 [Caenorhabditis briggsae] emb|CAE57042.1| Hypothetical protein CBG24928 [Caenorhabditis briggsae] 2.0E-42
blast XP_653447 Viral A-type inclusion protein repeat, putative [Entamoeba histolytica HM-1:IMSS] gb|EAL48061.1| Viral A-type inclusion protein repeat, putative [Entamoeba histolytica HM-1:IMSS] 3.0E-39
blast XP_653447 Viral A-type inclusion protein repeat, putative [Entamoeba histolytica HM-1:IMSS] gb|EAL48061.1| Viral A-type inclusion protein repeat, putative [Entamoeba histolytica HM-1:IMSS] 2.0E-36
Sequence
Target Sequence 
>C. ELEGANS:179200_AT
gaatcggctgatctgaagtctctggaagagttggaacagttaaaagaagaacttcgcatt
gtttcggagcagaatggattgcttaaggattcggaagctcgattattggatcacgctgac
gagttcgccgttcaaatggacaagtatcgtgagaaatgcgaagttctgacagcaaaaatt
gcagaactggaagcacaacttcagaatcctgctgaagaagatcaacagcagaagacatca
aacgtcgagctagtcaagctgagacaacaacttgcaaatgctcaacaggatatgcgagtt
caaaatctgacgatttctgatcgtgaaggtcttatcgcggagtatcgtcatcaaattgcc
gagcagaccaagacaattgaagaacttcacttgaaactaaaaaccttctcagcggcggct
cgtcgaacttctccacgagtgccaatgtcatcgaatgtaccagctctctcatcccgccca
tcatccagaatgtcaacgtcgtcacgaatctcaaccgcgtcttcgtttgtgccagaatcc
gtggcagtaccactacaatcaactggatcagcgtttgatgtcgtcgtac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCGGCTGATCTGAAGTCTCTGG 510 329 6385 Antisense
GAACTTCGCATTGTTTCGGAGCAGA 319 349 6433 Antisense
TATTGGATCACGCTGACGAGTTCGC 478 665 6488 Antisense
CGAGTTCGCCGTTCAAATGGACAAG 368 249 6504 Antisense
GGTCTTATCGCGGAGTATCGTCATC 240 503 6712 Antisense
AACTTCTCCACGAGTGCCAATGTCA 411 139 6810 Antisense
TCATCGAATGTACCAGCTCTCTCAT 334 621 6832 Antisense
CGCCCATCATCCAGAATGTCAACGT 539 255 6859 Antisense
CAACGTCGTCACGAATCTCAACCGC 657 187 6878 Antisense
TCTTCGTTTGTGCCAGAATCCGTGG 204 617 6904 Antisense
GGATCAGCGTTTGATGTCGTCGTAC 480 511 6949 Antisense
Sequence Source Affymetrix Proprietary Database