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[179181_at]

Description T27F6.4 /REP_DB=WormBase Gene ID /WP=CE16513 /TR=O45868 /GB=CAB04883.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 79      4grp_total > 18      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 8.6
(-3)
A 		8.6
(-3)
A 		16.9
(-1)
A 		33.3
(3)
A 		25
B 8
(-2.2)
A 		23
(1.6)
A 		5
(-3)
A 		28.3
(3)
A 		23
C 11.7
(-2.7)
A 		17.1
(-1.6)
A 		10.1
(-3)
A 		40
(3)
A 		30
avr 9.4
(-3) 		16.2
(-1.3) 		10.7
(-2.7) 		33.9
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 7.7
(-2.1)
A 		8
(-2)
A 		9.6
(-1.5)
A 		4.9
(-3)
A 		23.1
(3)
A 		17
(1)
A 		18
B 9.5
(-2.5)
A 		9.5
(-2.5)
A 		6.2
(-3)
A 		9.2
(-2.5)
A 		28.6
(0.4)
A 		45.8
(3)
A 		40
C 20.7
(-1.6)
A 		12.1
(-2.6)
A 		8.5
(-3)
A 		8.7
(-3)
A 		16.1
(-2.1)
A 		60
(3)
A 		52
avr 12.6
(-2.1) 		9.9
(-2.6) 		8.1
(-2.9) 		7.6
(-3) 		22.6
(-0.3) 		40.9
(3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 3.1
(-0.6)
A 		1.9
(-3)
A 		4.4
(2)
A 		4.9
(3)
A 		3
B 2.6
(-1.3)
A 		5.7
(2.6)
A 		1.2
(-3)
A 		6
(3)
A 		5
C 3.9
(-2.3)
A 		4.9
(-0.4)
A 		3.5
(-3)
A 		6.7
(3)
A 		3
avr 3.2
(-2.6) 		4.2
(-0.5) 		3
(-3) 		5.9
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 1.4
(-2.8)
A 		2.9
(-1.2)
A 		2.1
(-2.1)
A 		1.2
(-3)
A 		7
(3)
A 		4.1
(-0)
A 		6
B 3.3
(-2.3)
A 		3.2
(-2.3)
A 		1.5
(-3)
A 		2.4
(-2.7)
A 		11.6
(0.9)
A 		17.1
(3)
A 		16
C 7.7
(-1)
A 		3.7
(-2.3)
A 		2.2
(-2.8)
A 		1.7
(-3)
A 		5.2
(-1.9)
A 		20.1
(3)
A 		18
avr 4.1
(-1.9) 		3.3
(-2.3) 		1.9
(-3) 		1.8
(-3) 		7.9
(0.1) 		13.8
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179181_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1342
Sequence Type Exemplar sequence
Representative Public ID T27F6.4 NCBI
Target Description T27F6.4 /REP_DB=WormBase Gene ID /WP=CE16513 /TR=O45868 /GB=CAB04883.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060766(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060766 NCBI Caenorhabditis elegans T27F6.4 (T27F6.4) mRNA, complete cds. 11/11 None
SNAP00000032599 ENSEMBL cdna:SNAP chromosome:CEL140:I:12490126:12494278:1 11/11 None
T27F6.4 ENSEMBL cdna:known chromosome:CEL140:I:12492020:12494524:1 gene:T27F6.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000032610 8/11 Cross Hyb Matching Probes None
GENEFINDER00000032607 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12492268-12494209(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T27F6.4
Entrez Gene 173116 Entrez gene
SwissProt O45868 EMBL-EBI
WormBase CE16513 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04883 Hypothetical protein T27F6.4 [Caenorhabditis elegans] ref|NP_493167.1| T27F6.4 [Caenorhabditis elegans] 1.0E-75
blast CAB04883 Hypothetical protein T27F6.4 [Caenorhabditis elegans] ref|NP_493167.1| T27F6.4 [Caenorhabditis elegans] 2.0E-75
blast CAE60707 Hypothetical protein CBG04373 [Caenorhabditis briggsae] emb|CAE60706.1| Hypothetical protein CBG04372 [Caenorhabditis briggsae] 1.0E-9
Sequence
Target Sequence 
>C. ELEGANS:179181_AT
atgccattccattatccaaaactctacatggatcaccgcggaaacatgtccacctcatcg
tctctgacgtcatcgacaaccgccacgtcatcatcgtcaaccggaggcgtccgcaagcag
attctcacgttgaagccaaagcaaccgaatggttatgtgcaacacttggagatcaaggca
aaggaatcaagccaatcttcggtgatttccggcggtgtcccggttccggtgaccctgttg
gagttgaagaagaaggatcgtagcgagaagaatgtggtgaccaacctgaagtatcgtaag
ccaacggcgaatcatcatctcgattcggtgcattcttcgtcggagcaccatcaccaccac
catctgagcctgagctctttgacgcgccgcccgggatctccgtcctcgtccggttacgag
actgactcgctcgtcgatcaggagaattgcgacgttttttcgagtctcttcaactcgtcg
ctcagctaccaatcggctctcgaccatcatcatcagtttcacgagctcgacatgcttcac
tcgcatccgatttggtctccacagttaccgaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCATTCCATTATCCAAAACTCT 418 41 13 Antisense
AAACTCTACATGGATCACCGCGGAA 253 127 31 Antisense
CCTCATCGTCTCTGACGTCATCGAC 704 265 65 Antisense
ATCTTCGGTGATTTCCGGCGGTGTC 708 13 207 Antisense
GCCAACGGCGAATCATCATCTCGAT 258 279 312 Antisense
TCGATTCGGTGCATTCTTCGTCGGA 535 603 332 Antisense
CCACCATCTGAGCCTGAGCTCTTTG 572 273 369 Antisense
TACGAGACTGACTCGCTCGTCGATC 85 649 427 Antisense
TGCGACGTTTTTTCGAGTCTCTTCA 703 581 460 Antisense
GTTTCACGAGCTCGACATGCTTCAC 465 449 528 Antisense
GATTTGGTCTCCACAGTTACCGAAC 476 427 561 Antisense
Sequence Source Affymetrix Proprietary Database