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[179166_s_at]

Description R148.5 /REP_DB=WormBase Gene ID /WP=CE12856 /TR=O17265 /GB=AAB71039.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 08      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 50      6grp_total > 19      4grp_total > 51      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 502
1 2 3 4 range
A 416.7
(-0.3)
P 		670.7
(3)
P 		495.6
(0.7)
P 		212.1
(-3)
P 		459
B 360
(-1)
P 		586.6
(3)
P 		500.4
(1.5)
P 		249.7
(-3)
P 		337
C 404.8
(-0.8)
P 		714.5
(3)
P 		524.4
(0.7)
P 		220.2
(-3)
P 		494
avr 393.8
(-0.7) 		657.3
(3) 		506.8
(0.9) 		227.3
(-3) 		430

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 483
1 2 3 4 5 6 range
A 705.8
(3)
P 		584.9
(0.6)
P 		409.6
(-3)
P 		581.5
(0.5)
P 		515.6
(-0.9)
P 		422.5
(-2.7)
P 		296
B 588.4
(3)
P 		471.8
(0.5)
P 		441.7
(-0.2)
P 		562.6
(2.4)
P 		485.2
(0.8)
P 		310.8
(-3)
P 		278
C 740.4
(3)
P 		428.7
(-0.9)
P 		516.2
(0.2)
P 		468.3
(-0.4)
P 		605.3
(1.3)
P 		257
(-3)
P 		483
avr 678.2
(3) 		495.1
(-0.2) 		455.8
(-0.8) 		537.5
(0.6) 		535.4
(0.5) 		330.1
(-3) 		348
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 151.7
(3)
P 		145.6
(2.7)
P 		129.6
(1.9)
P 		31
(-3)
P 		121
B 114.8
(1)
P 		144.9
(3)
P 		116
(1.1)
P 		53.2
(-3)
P 		92
C 134
(0.5)
P 		205.4
(3)
P 		182.8
(2.2)
P 		37.1
(-3)
P 		168
avr 133.5
(1.5) 		165.3
(3) 		142.8
(1.9) 		40.4
(-3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 188
1 2 3 4 5 6 range
A 130.1
(-0.9)
P 		208
(3)
P 		89.2
(-3)
P 		147.5
(-0.1)
P 		155.6
(0.4)
P 		102.9
(-2.3)
P 		119
B 204.7
(3)
P 		157
(0.1)
P 		107.3
(-3)
P 		149
(-0.4)
P 		197.1
(2.5)
P 		115.9
(-2.5)
P 		97
C 274.4
(3)
P 		130.8
(-1.6)
P 		131.4
(-1.6)
P 		94.2
(-2.7)
P 		195.9
(0.5)
P 		86
(-3)
P 		188
avr 203.1
(3) 		165.3
(0.8) 		109.3
(-2.5) 		130.2
(-1.3) 		182.9
(1.8) 		101.6
(-3) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179166_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7781
Sequence Type Exemplar sequence
Representative Public ID R148.5 NCBI
Target Description R148.5 /REP_DB=WormBase Gene ID /WP=CE12856 /TR=O17265 /GB=AAB71039.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065264(11), NM_181961(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065264 NCBI Caenorhabditis elegans R148.5a (R148.5) mRNA, complete cds. 11/11 None
NM_181961 NCBI Caenorhabditis elegans R148.5b (R148.5) mRNA, complete cds. 11/11 None
SNAP00000030364 ENSEMBL cdna:SNAP chromosome:CEL140:III:3166274:3180754:1 11/11 None
GENEFINDER00000030375 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3166274:3170926:1 11/11 None
R148.5a ENSEMBL cdna:known chromosome:CEL140:III:3166274:3171187:1 gene:R148.5 11/11 None
R148.5b ENSEMBL cdna:known chromosome:CEL140:III:3166274:3170573:1 gene:R148.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3166081-3170926(+) 95.95 100.0
Public Domain and Genome References
Ensembl R148.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN65300 Hypothetical protein R148.3b [Caenorhabditis elegans] ref|NP_871687.1| R148.3b [Caenorhabditis elegans] 0.0
blast AAB71038 Hypothetical protein R148.3a [Caenorhabditis elegans] ref|NP_497667.1| R148.3a [Caenorhabditis elegans] 0.0
blast CAE74671 Hypothetical protein CBG22473 [Caenorhabditis briggsae] 1.0E-157
blast AAN65301 Hypothetical protein R148.5b [Caenorhabditis elegans] ref|NP_871690.1| R148.5b [Caenorhabditis elegans] 1.0E-144
blast AAB71039 Hypothetical protein R148.5a [Caenorhabditis elegans] ref|NP_497665.2| R148.5a [Caenorhabditis elegans] 1.0E-144
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 0.389999985694885
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000030364 4 498-520,540-562,577-599,1151-1170
Sequence
Target Sequence 
>C. ELEGANS:179166_S_AT
ctccaccaccacatttcggtatgggaccaccacgtggatttatgccgcccccgcatccaa
tgatgttccgaggtggaccatacccgccgttctttcctccaccaccaccacatttcatga
ggcctagtagcccaccgactgatggtggaccaattattactgggccagagtcggtttatg
ggacaatgcgtcgaggagcttatgaagagccagcttatgttggagcaactggaggattcc
cggaggctagctatcaaccggctaattatggtggagatggttatgagacatattatgata
cattcaagaagcgaggaacactgaagaagggggaatcggcgtcgatggcttccacgagag
ccgatagcgcctgggatcaatatgaagctgggatctatagaaaaccacatatcaatgaga
aagctttttcgggaactttgagatcaatggccgccaaggagacaagtaacgctacgctga
atcgatcggagcacgccgaagttgcatcgcaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCACCACCACATTTCGGTATGGG 465 235 1169 Antisense
CCCCCGCATCCAATGATGTTCCGAG 128 261 1216 Antisense
TGATGTTCCGAGGTGGACCATACCC 368 563 1229 Antisense
CACCACCACATTTCATGAGGCCTAG 225 203 1271 Antisense
CCTAGTAGCCCACCGACTGATGGTG 644 267 1291 Antisense
GCAACTGGAGGATTCCCGGAGGCTA 480 319 1393 Antisense
GGAGGCTAGCTATCAACCGGCTAAT 540 515 1410 Antisense
AAGGGGGAATCGGCGTCGATGGCTT 77 161 1495 Antisense
GGCTTCCACGAGAGCCGATAGCGCC 172 533 1515 Antisense
GTAACGCTACGCTGAATCGATCGGA 522 459 1634 Antisense
AGCACGCCGAAGTTGCATCGCAAAC 241 77 1658 Antisense
Sequence Source Affymetrix Proprietary Database