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[179161_at] adenylyl cyclase

Description C44F1.4 /REP_DB=WormBase Gene ID /WP=CE02164 /TR=Q18626 /GB=CAA88851.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 22      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 70      6grp_total > 13      4grp_total > 45      6grp_highest_sub > 02      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 299
1 2 3 4 range
A 531.2
(3)
P 		470.2
(1.7)
P 		264.6
(-2.5)
P 		241.3
(-3)
P 		290
B 474.7
(3)
P 		303.4
(-1.9)
P 		294.5
(-2.1)
P 		263.7
(-3)
P 		211
C 433.1
(3)
P 		336.2
(0.1)
P 		331.9
(-0)
P 		232.2
(-3)
P 		201
avr 479.7
(3) 		369.9
(0.2) 		297
(-1.7) 		245.7
(-3) 		234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 302
1 2 3 4 5 6 range
A 237.2
(-0.2)
P 		374.3
(3)
P 		263
(0.4)
P 		316.2
(1.7)
P 		183.2
(-1.4)
P 		114.8
(-3)
P 		260
B 402.4
(2.7)
P 		416.3
(3)
P 		372.8
(2.1)
P 		321
(1)
P 		188
(-1.7)
P 		123.2
(-3)
P 		293
C 390.8
(3)
P 		368.5
(2.5)
P 		372.8
(2.6)
P 		381.2
(2.8)
P 		268.5
(0)
P 		145.4
(-3)
P 		245
avr 343.5
(2) 		386.4
(3) 		336.2
(1.8) 		339.5
(1.9) 		213.2
(-1) 		127.8
(-3) 		259
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 193.4
(3)
P 		102.1
(-0.5)
P 		69.2
(-1.7)
P 		35.3
(-3)
P 		158
B 151.4
(3)
P 		74.9
(-1.8)
P 		68.2
(-2.2)
P 		56.2
(-3)
P 		95
C 143.4
(3)
P 		96.7
(0.3)
P 		115.7
(1.4)
P 		39.1
(-3)
P 		104
avr 162.7
(3) 		91.2
(-0.6) 		84.4
(-0.9) 		43.5
(-3) 		119

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 43.7
(-2.1)
P 		133.1
(3)
P 		57.3
(-1.3)
P 		80.2
(-0)
P 		55.3
(-1.4)
P 		28
(-3)
P 		105
B 140
(3)
P 		138.6
(2.9)
P 		90.5
(-0.2)
P 		85
(-0.5)
P 		76.4
(-1.1)
P 		45.9
(-3)
P 		94
C 144.8
(3)
P 		112.4
(1)
P 		94.9
(-0.1)
P 		76.7
(-1.3)
P 		86.9
(-0.6)
P 		48.7
(-3)
P 		96
avr 109.5
(1.7) 		128
(3) 		80.9
(-0.2) 		80.6
(-0.3) 		72.9
(-0.8) 		40.9
(-3) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179161_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6606
Sequence Type Exemplar sequence
Representative Public ID C44F1.4 NCBI
Target Description C44F1.4 /REP_DB=WormBase Gene ID /WP=CE02164 /TR=Q18626 /GB=CAA88851.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065364(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065364 NCBI Caenorhabditis elegans Adenylyl CYclase family member (acy-3) (acy-3) mRNA, complete cds. 11/11 None
SNAP00000024034 ENSEMBL cdna:SNAP chromosome:CEL140:III:3775149:3779732:-1 11/11 None
GENEFINDER00000024037 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3775149:3779761:-1 11/11 None
C44F1.5 ENSEMBL cdna:known chromosome:CEL140:III:3774871:3781875:-1 gene:C44F1.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3775148-3776897(-) 100.0 100.0
Public Domain and Genome References
Gene Title adenylyl cyclase
Gene Symbol acy-3
Ensembl C44F1.5
Entrez Gene 175489 Entrez gene
SwissProt Q18628 EMBL-EBI
WormBase CE33567 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5886 plasma membrane inferred from curator QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4016 adenylate cyclase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88853 Hypothetical protein C44F1.5 [Caenorhabditis elegans] emb|CAA88885.2| Hypothetical protein C44F1.5 [Caenorhabditis elegans] ref|NP_497765.2| Adenylyl CYclase family member (acy-3) [Caenorhabditis elegans] 0.0
blast CAE64497 Hypothetical protein CBG09223 [Caenorhabditis briggsae] 0.0
blast T19934 hypothetical protein C44F1.4 - Caenorhabditis elegans 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 5.8E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497765.2 5 7-29,349-371,376-398,413-435,465-487
C44F1.5 5 7-29,349-371,376-398,413-435,465-487
SNAP00000024034 3 5-27,42-64,94-116
GENEFINDER00000024037 3 13-35,50-72,104-126
Sequence
Target Sequence 
>C. ELEGANS:179161_AT
tcgctccgaaacggttttggcctattccgaaaaggccactctgataacgaacaatcagtt
gatggaattgataaaatagaggcagctgcaaatcgagttgatcgaatgattcaagaactc
aacgcttttggagatttcgctgagatcaaaccactcgaatatcaaccatttcctgcaaat
ttcggatcagtaaaatcgatacatcgtgcaatgtcatcagcatgtcatacagaatacgat
aatgcagaatctgatgctgttatctctgatgtagagtcgaatgctaactcgatttctcag
ttaagaaattctaaatctcgacagaaacgtcgatggaaagaagcaagaagatggcatcgc
gatgctgctgatggtgatactgaatcacaatgtagtagtatggctggaagtattgattta
gatcctatcagatggaaatcaactcattcaatcggttacgaaaacgagtatgaaatgcaa
agtgacgttgagggactggcatttgaagaaatggctgccctttctcgtgatattcgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGCTCCGAAACGGTTTTGGCCTAT 352 599 343 Antisense
CTATTCCGAAAAGGCCACTCTGATA 474 223 364 Antisense
GAGGCAGCTGCAAATCGAGTTGATC 165 405 421 Antisense
CAACGCTTTTGGAGATTTCGCTGAG 571 187 462 Antisense
GATTTCGCTGAGATCAAACCACTCG 293 427 475 Antisense
CACTCGAATATCAACCATTTCCTGC 453 197 494 Antisense
GTGCAATGTCATCAGCATGTCATAC 412 481 548 Antisense
GAATCTGATGCTGTTATCTCTGATG 507 329 589 Antisense
GCGATGCTGCTGATGGTGATACTGA 558 289 701 Antisense
GTGACGTTGAGGGACTGGCATTTGA 212 481 824 Antisense
CTGCCCTTTCTCGTGATATTCGAAA 477 237 857 Antisense
Sequence Source Affymetrix Proprietary Database