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[179146_at]

Description T28A8.4 /REP_DB=WormBase Gene ID /WP=CE18975 /TR=Q9XU05 /GB=CAB07288.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 03      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 68.5
(3)
A 		22.7
(-3)
A 		54
(1.1)
A 		40.5
(-0.7)
A 		46
B 44.4
(-3)
A 		61.9
(-1.8)
A 		51
(-2.6)
A 		134.5
(3)
A 		90
C 56.5
(-2.7)
A 		57.6
(-2.6)
A 		53.4
(-3)
A 		115.1
(3)
A 		62
avr 56.5
(-1.9) 		47.4
(-3) 		52.8
(-2.3) 		96.7
(3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 409
1 2 3 4 5 6 range
A 94.5
(-2.1)
A 		76.3
(-3)
A 		89.8
(-2.3)
P 		91.9
(-2.2)
A 		180.9
(2.2)
P 		198
(3)
P 		122
B 43
(-3)
A 		75.6
(-2.5)
A 		114.1
(-1.9)
A 		119.8
(-1.8)
P 		197.5
(-0.6)
P 		428
(3)
P 		385
C 56
(-2.7)
A 		87.6
(-2.3)
A 		36.7
(-3)
A 		102.9
(-2)
A 		165.9
(-1.1)
P 		445.3
(3)
P 		409
avr 64.5
(-3) 		79.8
(-2.7) 		80.2
(-2.7) 		104.9
(-2.2) 		181.4
(-0.6) 		357.1
(3) 		293
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 24.9
(3)
A 		4.9
(-3)
A 		14.1
(-0.2)
A 		5.9
(-2.7)
A 		20
B 14.2
(-2.1)
A 		15.3
(-1.8)
A 		11.8
(-3)
A 		28.7
(3)
A 		17
C 18.7
(1.5)
A 		16.6
(-3)
A 		18.6
(1.3)
A 		19.4
(3)
A 		3
avr 19.3
(3) 		12.3
(-3) 		14.8
(-0.9) 		18
(1.9) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 17.4
(-3)
A 		27.1
(-1.4)
A 		19.6
(-2.6)
P 		23.3
(-2)
A 		54.6
(3)
P 		48.2
(2)
P 		37
B 15
(-3)
A 		25.2
(-2.6)
A 		27.7
(-2.5)
A 		31.7
(-2.3)
P 		80.2
(-0.3)
P 		159.6
(3)
P 		145
C 20.7
(-2.5)
A 		26.7
(-2.3)
A 		9.3
(-3)
A 		20.7
(-2.5)
A 		53.7
(-1.1)
P 		149.1
(3)
P 		140
avr 17.7
(-3) 		26.3
(-2.5) 		18.9
(-2.9) 		25.2
(-2.6) 		62.8
(-0.3) 		119
(3) 		101
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179146_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7864
Sequence Type Exemplar sequence
Representative Public ID T28A8.4 NCBI
Target Description T28A8.4 /REP_DB=WormBase Gene ID /WP=CE18975 /TR=Q9XU05 /GB=CAB07288.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067393(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067393 NCBI Caenorhabditis elegans T28A8.4 (T28A8.4) mRNA, complete cds. 11/11 None
SNAP00000022263 ENSEMBL cdna:SNAP chromosome:CEL140:III:13501146:13504954:1 11/11 None
GENEFINDER00000022275 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13503206:13504954:1 11/11 None
T28A8.4 ENSEMBL cdna:known chromosome:CEL140:III:13503206:13504954:1 gene:T28A8.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13503201-13504950(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28A8.4
Entrez Gene 176781 Entrez gene
SwissProt Q9XU05 EMBL-EBI
WormBase CE18975 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07288 Hypothetical protein T28A8.4 [Caenorhabditis elegans] ref|NP_499794.1| T28A8.4 [Caenorhabditis elegans] 0.0
blast CAB07285 Hypothetical protein T28A8.5 [Caenorhabditis elegans] ref|NP_499793.1| T28A8.5 [Caenorhabditis elegans] 1.0E-130
blast CAB55012 Hypothetical protein Y57A10A.7 [Caenorhabditis elegans] ref|NP_496587.1| Y57A10A.7 [Caenorhabditis elegans] 2.0E-98
blast CAB55010 Hypothetical protein Y57A10A.5 [Caenorhabditis elegans] ref|NP_496585.1| Y57A10A.5 [Caenorhabditis elegans] 3.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR006570 EMBL-EBI SPK 3.2E-62
Pfam IPR006570 EMBL-EBI SPK 4.3E-65
Pfam IPR006570 EMBL-EBI SPK 2.0E-66
Pfam IPR006570 EMBL-EBI SPK 3.1E-45
Pfam IPR006570 EMBL-EBI SPK 1.4E-55
Pfam IPR006570 EMBL-EBI SPK 4.3E-65
Sequence
Target Sequence 
>C. ELEGANS:179146_AT
gagcggtggaaggacagcccaaggctgaaccgattgatgcaccatataattataataccg
atcatttgaacgaagatccttcgatttccaagaatgaaagagttattccagttgaatcga
agatcataagcttgcacgactttctgaaacagctgacccaattcatttgcattctcgaga
gtttggagctcgaagagctcaagcaacaaatcaaagaatcgattagcactgatgatgatg
agagcctgcaaatcatcgatatttgtgcagtactcgagacattctgcttcgggatcagtc
gaaaaaccagagtagccgcttcaaatatcccagctatgaaggtcaaggatttcctgcttg
cgttcaaattcttccttctctggatggattcttcggagcttcgagagcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCGGTGGAAGGACAGCCCAAGGC 244 385 899 Antisense
AAGGCTGAACCGATTGATGCACCAT 664 161 919 Antisense
GAAGATCCTTCGATTTCCAAGAATG 592 337 970 Antisense
GATCATAAGCTTGCACGACTTTCTG 515 419 1020 Antisense
ACCCAATTCATTTGCATTCTCGAGA 506 89 1054 Antisense
GAGAGCCTGCAAATCATCGATATTT 571 393 1138 Antisense
GTACTCGAGACATTCTGCTTCGGGA 665 459 1168 Antisense
AGTAGCCGCTTCAAATATCCCAGCT 346 63 1209 Antisense
GATTTCCTGCTTGCGTTCAAATTCT 634 425 1246 Antisense
AATTCTTCCTTCTCTGGATGGATTC 372 177 1265 Antisense
GATTCTTCGGAGCTTCGAGAGCTTC 482 431 1285 Antisense
Sequence Source Affymetrix Proprietary Database