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[179144_at]

Description T25B9.3 /REP_DB=WormBase Gene ID /WP=CE06502 /TR=Q22767 /GB=CAA94375.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 13      4grp_highest_8_8 > 61      4grp_high_8_8 > 17      6grp_highest_nonorm > 71      6grp_total > 14      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 307
1 2 3 4 range
A 392.7
(3)
P 		213.8
(-1.8)
P 		171.2
(-3)
P 		318.6
(1)
P 		222
B 391.9
(3)
P 		222.4
(-1.3)
P 		152.9
(-3)
P 		323.3
(1.3)
P 		239
C 460.2
(3)
P 		291.6
(-0.5)
P 		175
(-3)
P 		325.8
(0.2)
P 		285
avr 414.9
(3) 		242.6
(-1.2) 		166.4
(-3) 		322.6
(0.8) 		249

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 334
1 2 3 4 5 6 range
A 429.8
(3)
P 		335.5
(0.8)
P 		406.3
(2.4)
P 		341.5
(0.9)
P 		180.3
(-2.9)
P 		176.6
(-3)
P 		253
B 485.6
(3)
P 		352
(0.1)
P 		265.8
(-1.8)
P 		334.7
(-0.3)
P 		210.5
(-3)
P 		225.9
(-2.7)
P 		275
C 376.5
(0.1)
P 		511
(3)
P 		280.4
(-2)
P 		386.3
(0.3)
P 		239.5
(-2.9)
P 		236.5
(-3)
P 		275
avr 430.6
(3) 		399.5
(2.2) 		317.5
(-0.1) 		354.2
(0.9) 		210.1
(-3) 		213
(-2.9) 		221
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 143
(3)
P 		46.4
(-2.9)
P 		44.8
(-3)
P 		46.6
(-2.9)
P 		98
B 125
(3)
P 		54.9
(-1.7)
P 		35.4
(-3)
P 		68.9
(-0.8)
P 		90
C 152.3
(3)
P 		83.8
(-1.2)
P 		61
(-2.6)
P 		54.9
(-3)
P 		97
avr 140.1
(3) 		61.7
(-2.1) 		47.1
(-3) 		56.8
(-2.4) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 79.2
(-0.2)
P 		119.3
(3)
P 		88.5
(0.6)
P 		86.6
(0.4)
P 		54.4
(-2.1)
P 		43
(-3)
P 		76
B 169
(3)
P 		117.2
(0)
P 		64.5
(-3)
P 		88.7
(-1.6)
P 		85.5
(-1.8)
P 		84.2
(-1.9)
P 		105
C 139.6
(1.8)
P 		155.9
(3)
P 		71.4
(-3)
P 		77.7
(-2.6)
P 		77.5
(-2.6)
P 		79.2
(-2.4)
P 		85
avr 129.3
(2.9) 		130.8
(3) 		74.8
(-2.4) 		84.3
(-1.5) 		72.5
(-2.6) 		68.8
(-3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179144_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8435
Sequence Type Exemplar sequence
Representative Public ID T25B9.3 NCBI
Target Description T25B9.3 /REP_DB=WormBase Gene ID /WP=CE06502 /TR=Q22767 /GB=CAA94375.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028231(9), NM_001028232(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028231 NCBI Caenorhabditis elegans T25B9.3a (T25B9.3) mRNA, complete cds. 9/11 None
NM_001028232 NCBI Caenorhabditis elegans T25B9.3b (T25B9.3) mRNA, complete cds. 10/11 None
GENEFINDER00000044128 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10746058:10748225:1 9/11 None
T25B9.3a ENSEMBL cdna:known chromosome:CEL140:IV:10744753:10748247:1 gene:T25B9.3 9/11 None
T25B9.3b ENSEMBL cdna:known chromosome:CEL140:IV:10746081:10748225:1 gene:T25B9.3 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000044109 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10746063-10748231(+) 100.0 100.0
Public Domain and Genome References
Ensembl T25B9.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAH04716 Hypothetical protein T25B9.3a [Caenorhabditis elegans] emb|CAA94375.2| Hypothetical protein T25B9.3a [Caenorhabditis elegans] ref|NP_001023402.1| T25B9.3a [Caenorhabditis elegans] 1.0E-127
blast CAH04716 Hypothetical protein T25B9.3a [Caenorhabditis elegans] emb|CAA94375.2| Hypothetical protein T25B9.3a [Caenorhabditis elegans] ref|NP_001023402.1| T25B9.3a [Caenorhabditis elegans] 4.0E-84
blast CAE70861 Hypothetical protein CBG17649 [Caenorhabditis briggsae] 8.0E-78
blast CAE70861 Hypothetical protein CBG17649 [Caenorhabditis briggsae] 1.0E-77
blast CAH04719 Hypothetical protein T25B9.3b [Caenorhabditis elegans] ref|NP_001023403.1| T25B9.3b [Caenorhabditis elegans] 2.0E-68
blast CAH04716 Hypothetical protein T25B9.3a [Caenorhabditis elegans] emb|CAA94375.2| Hypothetical protein T25B9.3a [Caenorhabditis elegans] ref|NP_001023402.1| T25B9.3a [Caenorhabditis elegans] 2.0E-68
Sequence
Target Sequence 
>C. ELEGANS:179144_AT
aggatatttgattctcctctcgttcgatggtttgaagcctgacacatggtttgctgtacg
tgtagaataccggcttaactttgctgacgacgcagctgacaacgcattgagaatgacaaa
acaggaaatggtcgtgaaaacaaaaaagacccctcgtgatgatccaaagaaaattgagca
aatggtagcattcatcgatgatctgtttgtaacaaaagataatatttatatcggtgttgg
atcagttttcaaagaaataaaaaagatttcaacggtcattgttccggaattaagatgctc
aaagggagtgttgagtccagtgtcacaacaagttatccagcatgcttcatttcattttga
tttatcaaaacttccaaaagaagatagaaagtgtagcacgatttgcgtattcccatatct
aaggattttaacggaaaatgctgtaactgaaacattccgagcgaaagaatggtgtggaag
tgcagatgaagctcgacatttgctcactagtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGATATTTGATTCTCCTCTCGTTC 525 57 36 Antisense
TTCTCCTCTCGTTCGATGGTTTGAA 220 693 47 Antisense
GCCTGACACATGGTTTGCTGTACGT 449 283 72 Antisense
ATACCGGCTTAACTTTGCTGACGAC 558 21 102 Antisense
TGACGACGCAGCTGACAACGCATTG 4 569 120 Antisense
AAAAAAGACCCCTCGTGATGATCCA 691 127 177 Antisense
GATTTCAACGGTCATTGTTCCGGAA 327 427 300 Antisense
GGGAGTGTTGAGTCCAGTGTCACAA 590 495 339 Antisense
AAGTTATCCAGCATGCTTCATTTCA 187 157 365 Antisense
GCACGATTTGCGTATTCCCATATCT 629 315 431 Antisense
GAAGCTCGACATTTGCTCACTAGTC 452 343 523 Antisense
Sequence Source Affymetrix Proprietary Database