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[179081_at]

Description F35E8.7 /REP_DB=WormBase Gene ID /WP=CE15957 /TR=O45453 /GB=CAB04296.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 04      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 270
1 2 3 4 range
A 47.1
(-2)
A 		4.8
(-2.9)
A 		2.5
(-3)
A 		272
(3)
P 		270
B 58.9
(-1.6)
A 		3.7
(-3)
A 		3.8
(-3)
A 		240.9
(3)
P 		237
C 30.1
(-2.3)
A 		4.7
(-2.9)
A 		2.2
(-3)
A 		240.7
(3)
P 		239
avr 45.4
(-2) 		4.4
(-3) 		2.8
(-3) 		251.2
(3) 		248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 2.8
(-2.9)
A 		2.1
(-3)
A 		8
(-2.4)
A 		2.4
(-3)
A 		4.3
(-2.8)
A 		65.3
(3)
A 		63
B 2.6
(-2.9)
A 		2
(-3)
A 		5.6
(-2.6)
A 		3
(-2.9)
A 		2.4
(-3)
A 		63.7
(3)
A 		62
C 3.2
(-3)
A 		2.7
(-3)
A 		36.7
(-1.2)
A 		7.5
(-2.8)
A 		6.7
(-2.8)
A 		117.9
(3)
P 		115
avr 2.9
(-3) 		2.3
(-3) 		16.8
(-1.9) 		4.3
(-2.9) 		4.5
(-2.8) 		82.3
(3) 		80
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 17.1
(-0.5)
A 		1
(-3)
A 		0.7
(-3)
A 		39.8
(3)
P 		39
B 18.8
(-0.9)
A 		0.9
(-3)
A 		0.9
(-3)
A 		51.3
(3)
P 		50
C 10
(-1.6)
A 		1.3
(-2.9)
A 		0.8
(-3)
A 		40.6
(3)
P 		40
avr 15.3
(-1) 		1.1
(-3) 		0.8
(-3) 		43.9
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 0.5
(-3)
A 		0.7
(-2.9)
A 		1.7
(-2.5)
A 		0.6
(-3)
A 		1.3
(-2.7)
A 		15.9
(3)
A 		15
B 0.9
(-2.9)
A 		0.6
(-3)
A 		1.4
(-2.8)
A 		0.8
(-2.9)
A 		1
(-2.9)
A 		23.7
(3)
A 		23
C 1.2
(-2.9)
A 		0.8
(-3)
A 		9.3
(-1.7)
A 		1.5
(-2.9)
A 		2.2
(-2.8)
A 		39.5
(3)
P 		39
avr 0.9
(-3) 		0.7
(-3) 		4.1
(-2.2) 		1
(-2.9) 		1.5
(-2.8) 		26.4
(3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179081_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13658
Sequence Type Exemplar sequence
Representative Public ID F35E8.7 NCBI
Target Description F35E8.7 /REP_DB=WormBase Gene ID /WP=CE15957 /TR=O45453 /GB=CAB04296.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074581(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074581 NCBI Caenorhabditis elegans F35E8.7 (F35E8.7) mRNA, complete cds. 11/11 None
SNAP00000019513 ENSEMBL cdna:SNAP chromosome:CEL140:V:15916598:15917726:1 11/11 None
GENEFINDER00000019524 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15916598:15917726:1 11/11 None
F35E8.7 ENSEMBL cdna:known chromosome:CEL140:V:15916598:15917726:1 gene:F35E8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15916597-15917726(+) 85.7 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E8.7
Entrez Gene 185298 Entrez gene
SwissProt O45453 EMBL-EBI
WormBase CE15957 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04296 Hypothetical protein F35E8.7 [Caenorhabditis elegans] ref|NP_506982.1| F35E8.7 [Caenorhabditis elegans] 1.0E-130
blast CAB04295 Hypothetical protein F35E8.6 [Caenorhabditis elegans] ref|NP_506981.1| F35E8.6 [Caenorhabditis elegans] 1.0E-73
blast CAB04292 Hypothetical protein F35E8.9 [Caenorhabditis elegans] ref|NP_506984.1| F35E8.9 [Caenorhabditis elegans] 1.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.7E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.8E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.2E-10
Sequence
Target Sequence 
>C. ELEGANS:179081_AT
catacagctgcgagaatcctccgttgccaagtgtcaattgttctacaatcaaacctatcc
aatgcactgatccagcatggaaggcaattatagctatggaatgtccagctacgtgtggat
tgtgcagtgcagattcatgtgtcgatgcaattgaaggatgtgccaacaacatagaaatct
gtaatgcagtcaaattgcaagactttgtcaatgccaattgcagaaagacgtgtaaaagat
gtacgtcgggtcccactgcgacaacatctaccgcggctccggcggcacctacaactaccg
tggctcccgctgttgttacaactactgcggcccccgctgccgtgaccactactgctgcag
ttaccactactaccgtcgcagcagcagcagccacaaccacagcagccgcctgcacttcct
acattcccgacggccgaacaagatgcggcgagtatgctcgaagtggattctgtacaagca
accaatacaccacggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATACAGCTGCGAGAATCCTCCGTT 4 215 341 Antisense
AATCCTCCGTTGCCAAGTGTCAATT 80 169 355 Antisense
AACCTATCCAATGCACTGATCCAGC 602 147 392 Antisense
AGCTACGTGTGGATTGTGCAGTGCA 495 79 447 Antisense
GTGCAGTGCAGATTCATGTGTCGAT 342 481 462 Antisense
TAAAAGATGTACGTCGGGTCCCACT 533 627 573 Antisense
GCTGTTGTTACAACTACTGCGGCCC 530 291 649 Antisense
ACTACTGCTGCAGTTACCACTACTA 661 101 688 Antisense
CTACATTCCCGACGGCCGAACAAGA 511 225 759 Antisense
GAACAAGATGCGGCGAGTATGCTCG 392 353 776 Antisense
TACAAGCAACCAATACACCACGGCA 573 641 813 Antisense
Sequence Source Affymetrix Proprietary Database