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[179080_at]

Description C25G4.7 /REP_DB=WormBase Gene ID /WP=CE05308 /TR=Q18168 /GB=CAA94570.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 21      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 73.6
(0.6)
P 		52.6
(-2)
P 		43.9
(-3)
A 		93.9
(3)
A 		50
B 58.4
(1.2)
P 		25.3
(-3)
A 		49.3
(0.1)
M 		72.5
(3)
A 		47
C 68.3
(0.6)
P 		86.3
(2.2)
P 		28.9
(-3)
A 		95.4
(3)
P 		67
avr 66.8
(0.4) 		54.7
(-1.2) 		40.7
(-3) 		87.3
(3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 106.7
(3)
A 		37.6
(-3)
A 		81
(0.8)
P 		64.1
(-0.7)
A 		96
(2.1)
P 		106.6
(3)
P 		69
B 55.4
(-0.3)
P 		47.1
(-3)
P 		65.8
(3)
P 		55.8
(-0.2)
M 		65.2
(2.8)
P 		53.9
(-0.8)
P 		19
C 52.1
(-2.3)
P 		56.2
(-1.8)
P 		46.5
(-3)
A 		83.8
(1.6)
A 		95
(3)
P 		71.1
(0)
P 		49
avr 71.4
(0.8) 		47
(-3) 		64.4
(-0.3) 		67.9
(0.3) 		85.4
(3) 		77.2
(1.7) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 26.8
(3)
P 		11.4
(-3)
P 		11.5
(-3)
A 		13.7
(-2.1)
A 		15
B 18.6
(3)
P 		6.3
(-3)
A 		11.4
(-0.5)
M 		15.5
(1.5)
A 		12
C 22.6
(2.1)
P 		24.8
(3)
P 		10.1
(-3)
A 		16.1
(-0.6)
P 		15
avr 22.7
(3) 		14.2
(-1.4) 		11
(-3) 		15.1
(-0.9) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 19.7
(-0.6)
A 		13.4
(-3)
A 		17.6
(-1.4)
P 		16.3
(-1.9)
A 		29
(3)
P 		26
(1.8)
P 		16
B 19.3
(-0.7)
P 		15.7
(-2.5)
P 		16
(-2.4)
P 		14.8
(-3)
M 		26.5
(3)
P 		20.1
(-0.3)
P 		12
C 19.3
(-0.6)
P 		17.2
(-1.3)
P 		11.8
(-3)
A 		16.8
(-1.4)
A 		30.7
(3)
P 		23.8
(0.8)
P 		19
avr 19.4
(-1.1) 		15.4
(-2.9) 		15.1
(-3) 		16
(-2.6) 		28.7
(3) 		23.3
(0.6) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179080_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8896
Sequence Type Exemplar sequence
Representative Public ID C25G4.7 NCBI
Target Description C25G4.7 /REP_DB=WormBase Gene ID /WP=CE05308 /TR=Q18168 /GB=CAA94570.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069980(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069980 NCBI Caenorhabditis elegans C25G4.7 (C25G4.7) mRNA, complete cds. 11/11 None
SNAP00000033053 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12457164:12458532:1 11/11 None
GENEFINDER00000033063 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12457164:12458532:1 11/11 None
C25G4.7 ENSEMBL cdna:known chromosome:CEL140:IV:12457164:12458532:1 gene:C25G4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12457169-12458538(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25G4.7
Entrez Gene 182916 Entrez gene
SwissProt Q18168 EMBL-EBI
WormBase CE05308 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94570 Hypothetical protein C25G4.7 [Caenorhabditis elegans] ref|NP_502381.1| C25G4.7 [Caenorhabditis elegans] 1.0E-142
blast CAA94570 Hypothetical protein C25G4.7 [Caenorhabditis elegans] ref|NP_502381.1| C25G4.7 [Caenorhabditis elegans] 1.0E-135
blast AAF40012 Hypothetical protein ZK973.8 [Caenorhabditis elegans] ref|NP_491356.1| ZK973.8 [Caenorhabditis elegans] 8.0E-57
blast CAE60488 Hypothetical protein CBG04102 [Caenorhabditis briggsae] 2.0E-50
blast AAF40012 Hypothetical protein ZK973.8 [Caenorhabditis elegans] ref|NP_491356.1| ZK973.8 [Caenorhabditis elegans] 1.0E-48
blast CAE60488 Hypothetical protein CBG04102 [Caenorhabditis briggsae] 1.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR009676 EMBL-EBI Protein of unknown function DUF1265 1.7E-26
Pfam IPR009676 EMBL-EBI Protein of unknown function DUF1265 1.7E-26
Sequence
Target Sequence 
>C. ELEGANS:179080_AT
cacctatgagcctgacatcacggaccgtgacgatttcgactgcattacaccggaacgtgg
cgtgattcgagactttttgtgcgaggtgatcggaaatcggcaattcaagaaatacaaatt
ggaaattctcactgttatcgtatttgatatcaacaactttttgagcctgaaatacttcaa
tcgatttgttggaaaaccgatgccagtcgaattggatttcaaatattatctgttgatgaa
tcaaaagactcacaacttccttctcaccaccactactcaacaaggattcccgtgcaataa
agaagctctatacgtggcttctccatattttcgcaaccatctcacaagttccataaccag
ttttccactggaggtacaattcttggaagctattgaagtgaactgtgaggacttggatta
tgtggagaagttgctctttatggctcacactaattatctcggacgtcttctcgaagcctg
caacgccactattatcagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACCTATGAGCCTGACATCACGGAC 490 201 126 Antisense
GACGATTTCGACTGCATTACACCGG 287 379 154 Antisense
ACGTGGCGTGATTCGAGACTTTTTG 90 95 180 Antisense
TCAAAAGACTCACAACTTCCTTCTC 343 629 366 Antisense
CCACCACTACTCAACAAGGATTCCC 191 273 392 Antisense
GAAGCTCTATACGTGGCTTCTCCAT 560 341 427 Antisense
ATATTTTCGCAACCATCTCACAAGT 655 17 450 Antisense
CAAGTTCCATAACCAGTTTTCCACT 55 185 470 Antisense
GAAGTTGCTCTTTATGGCTCACACT 438 337 552 Antisense
GGCTCACACTAATTATCTCGGACGT 573 537 567 Antisense
AGCCTGCAACGCCACTATTATCAGT 1 85 600 Antisense
Sequence Source Affymetrix Proprietary Database