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[179074_at]

Description R04B5.8 /REP_DB=WormBase Gene ID /WP=CE06218 /TR=Q21705 /GB=CAA94844.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 12      4grp_total > 31      6grp_cgc > 33      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 179
1 2 3 4 range
A 151
(3)
M 		49.1
(-3)
A 		77.1
(-1.4)
A 		52.3
(-2.8)
A 		102
B 151.3
(3)
A 		9.2
(-3)
A 		37.4
(-1.8)
A 		107.7
(1.2)
A 		142
C 188.5
(3)
A 		15.8
(-3)
A 		62.8
(-1.4)
A 		96
(-0.2)
A 		173
avr 163.6
(3) 		24.7
(-3) 		59.1
(-1.5) 		85.3
(-0.4) 		139

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 104.2
(2)
A 		121.2
(3)
A 		81.5
(0.7)
A 		37.2
(-1.8)
A 		16.6
(-3)
A 		48.8
(-1.2)
A 		105
B 104.4
(1)
A 		128.1
(3)
A 		90.7
(-0.1)
A 		67.6
(-2.1)
A 		66.7
(-2.2)
A 		56.6
(-3)
A 		72
C 97.5
(2.2)
A 		110.8
(3)
A 		83.5
(1.4)
A 		106.8
(2.8)
A 		44.4
(-1)
A 		10.5
(-3)
A 		100
avr 102
(1.7) 		120
(3) 		85.2
(0.4) 		70.5
(-0.6) 		42.6
(-2.7) 		38.6
(-3) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 55
(3)
M 		10.7
(-2.6)
A 		20.1
(-1.4)
A 		7.7
(-3)
A 		47
B 48.2
(3)
A 		2.3
(-3)
A 		8.7
(-2.2)
A 		23
(-0.3)
A 		46
C 62.4
(3)
A 		4.6
(-3)
A 		21.9
(-1.2)
A 		16.2
(-1.8)
A 		58
avr 55.2
(3) 		5.9
(-3) 		16.9
(-1.7) 		15.6
(-1.8) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 19.2
(-0.8)
A 		43.1
(3)
A 		17.7
(-1)
A 		9.4
(-2.3)
A 		5
(-3)
A 		11.9
(-1.9)
A 		38
B 36.3
(1.5)
A 		42.6
(3)
A 		22
(-2)
A 		17.9
(-3)
A 		27.1
(-0.8)
A 		21.1
(-2.2)
A 		25
C 36.1
(3)
A 		33.8
(2.6)
A 		21.2
(0.3)
A 		21.5
(0.3)
A 		14.4
(-1)
A 		3.5
(-3)
A 		33
avr 30.5
(1) 		39.8
(3) 		20.3
(-1.2) 		16.3
(-2.1) 		15.5
(-2.3) 		12.2
(-3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179074_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11774
Sequence Type Exemplar sequence
Representative Public ID R04B5.8 NCBI
Target Description R04B5.8 /REP_DB=WormBase Gene ID /WP=CE06218 /TR=Q21705 /GB=CAA94844.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073193(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073193 NCBI Caenorhabditis elegans Serpentine Receptor, class D (delta) family member (srd-49) (srd-49) mRNA, complete cds. 11/11 None
GENEFINDER00000020547 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10097301:10098620:1 9/11 None
R04B5.8 ENSEMBL cdna:known chromosome:CEL140:V:10097301:10098892:1 gene:R04B5.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020540 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10097300-10098892(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srd-49
Ensembl R04B5.8
Entrez Gene 187569 Entrez gene
SwissProt Q21705 EMBL-EBI
WormBase CE06218 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94844 Hypothetical protein R04B5.8 [Caenorhabditis elegans] ref|NP_505594.1| Serpentine Receptor, class D (delta) family member (srd-49) [Caenorhabditis elegans] 0.0
blast CAE56921 Hypothetical protein CBG24766 [Caenorhabditis briggsae] 1.0E-162
blast CAA94844 Hypothetical protein R04B5.8 [Caenorhabditis elegans] ref|NP_505594.1| Serpentine Receptor, class D (delta) family member (srd-49) [Caenorhabditis elegans] 1.0E-160
blast CAE56921 Hypothetical protein CBG24766 [Caenorhabditis briggsae] 1.0E-144
blast CAA94133 Hypothetical protein F15A2.4 [Caenorhabditis elegans] ref|NP_510251.1| Serpentine Receptor, class D (delta) family member (srd-50) [Caenorhabditis elegans] sp|Q19474|SRD50_CAEEL Serpentine receptor class delta-50 (Protein srd-50) 4.0E-43
blast CAA94133 Hypothetical protein F15A2.4 [Caenorhabditis elegans] ref|NP_510251.1| Serpentine Receptor, class D (delta) family member (srd-50) [Caenorhabditis elegans] sp|Q19474|SRD50_CAEEL Serpentine receptor class delta-50 (Protein srd-50) 1.0E-42
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505594.1 7 10-32,45-64,100-117,130-152,186-208,233-255,270-292
R04B5.8 7 10-32,45-64,100-117,130-152,186-208,233-255,270-292
GENEFINDER00000020547 7 10-32,45-64,100-117,130-152,186-208,233-255,270-292
Sequence
Target Sequence 
>C. ELEGANS:179074_AT
gcatctacctgctatattctgtgcaatctgtcaatttataaatccatctcagcacaatgc
aattgttttggaaactcgtgctctgcatccgtcgtatatttttgagcaaaactcaatttt
tgggttttcagcgttgacgtctcctgcagtcaaagcatctactattattttcacaattgt
tttagtattaaacccactagctgcaattatctatcgcaacaagatatggggacttttaaa
tgaatacgaagaatacaaatctccacgaatcaaacatgcgaagagtatgatcacgggatt
gacaattcaaactttaattccaagtatttgttttgtaccattggtcgtacaattctttct
cacacaatattcggaagctggtgtgttaatactcgagtacttcaattcatttctggtaat
tctcccaactttgatagatccaattctatcaattgtatttgtaatacctttcaggcgaat
gttcttcaaatatcttctcgcagttcgccaatgcaggttcagctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATCTACCTGCTATATTCTGTGCA 455 309 423 Antisense
TGTTTTGGAAACTCGTGCTCTGCAT 249 561 486 Antisense
TGCTCTGCATCCGTCGTATATTTTT 248 581 501 Antisense
TTGGGTTTTCAGCGTTGACGTCTCC 52 607 542 Antisense
ACGTCTCCTGCAGTCAAAGCATCTA 242 95 559 Antisense
AACCCACTAGCTGCAATTATCTATC 94 147 613 Antisense
TCGTACAATTCTTTCTCACACAATA 143 605 767 Antisense
ACTCGAGTACTTCAATTCATTTCTG 389 99 813 Antisense
ATTTCTGGTAATTCTCCCAACTTTG 109 15 831 Antisense
TCAAATATCTTCTCGCAGTTCGCCA 357 629 908 Antisense
CAGTTCGCCAATGCAGGTTCAGCTT 613 205 923 Antisense
Sequence Source Affymetrix Proprietary Database