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[179064_at]

Description ZK218.8 /REP_DB=WormBase Gene ID /WP=CE16708 /TR=O46002 /GB=CAB04988.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 56      4grp_total > 0      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 123
1 2 3 4 range
A 26.1
(-1.8)
A 		6.8
(-3)
A 		49.3
(-0.3)
A 		101.4
(3)
A 		95
B 5.7
(-3)
A 		12.3
(-2.3)
A 		41.4
(0.5)
A 		66.3
(3)
A 		61
C 8.3
(-2.9)
A 		6.8
(-3)
A 		10.8
(-2.8)
A 		128.5
(3)
A 		122
avr 13.4
(-2.7) 		8.6
(-3) 		33.8
(-1.3) 		98.7
(3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 3.7
(-3)
A 		11.2
(-1.9)
A 		44.6
(3)
A 		7.4
(-2.5)
A 		8.4
(-2.3)
A 		6.9
(-2.5)
A 		41
B 5.5
(-2.8)
A 		7.8
(-2.3)
A 		4.5
(-3)
A 		22.7
(0.8)
A 		5.5
(-2.8)
A 		33
(3)
A 		29
C 8
(-3)
A 		75.9
(3)
A 		10
(-2.8)
A 		10.7
(-2.8)
A 		9.4
(-2.9)
A 		52.9
(1)
A 		68
avr 5.7
(-3) 		31.6
(3) 		19.7
(0.2) 		13.6
(-1.2) 		7.8
(-2.5) 		30.9
(2.8) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 9.5
(0.6)
A 		1.5
(-3)
A 		12.9
(2.1)
A 		14.8
(3)
A 		13
B 1.8
(-3)
A 		3
(-2.4)
A 		9.6
(0.8)
A 		14.1
(3)
A 		12
C 2.8
(-2.7)
A 		1.9
(-3)
A 		3.8
(-2.4)
A 		21.7
(3)
A 		20
avr 4.7
(-1.9) 		2.1
(-3) 		8.8
(-0.3) 		16.9
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 0.7
(-3)
A 		4
(-0.8)
A 		9.7
(3)
A 		1.9
(-2.2)
A 		2.5
(-1.8)
A 		1.7
(-2.3)
A 		9
B 1.9
(-2.6)
A 		2.6
(-2.2)
A 		1.1
(-3)
A 		6
(-0.4)
A 		2.2
(-2.4)
A 		12.3
(3)
A 		11
C 2.9
(-2.8)
A 		23.1
(3)
A 		2.5
(-2.9)
A 		2.2
(-3)
A 		3
(-2.8)
A 		17.7
(1.4)
A 		21
avr 1.8
(-3) 		9.9
(2.5) 		4.4
(-1.2) 		3.4
(-1.9) 		2.6
(-2.5) 		10.6
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 179064_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14340
Sequence Type Exemplar sequence
Representative Public ID ZK218.8 NCBI
Target Description ZK218.8 /REP_DB=WormBase Gene ID /WP=CE16708 /TR=O46002 /GB=CAB04988.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074936(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074936 NCBI Caenorhabditis elegans ZK218.8 (ZK218.8) mRNA, complete cds. 11/11 None
GENEFINDER00000041076 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17111706:17119307:1 11/11 None
ZK218.8 ENSEMBL cdna:known chromosome:CEL140:V:17118604:17119587:1 gene:ZK218.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041069 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17118603-17119587(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK218.8
Entrez Gene 191248 Entrez gene
SwissProt O46002 EMBL-EBI
WormBase CE16708 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04988 Hypothetical protein ZK218.8 [Caenorhabditis elegans] ref|NP_507337.1| ZK218.8 [Caenorhabditis elegans] 1.0E-106
blast CAB04988 Hypothetical protein ZK218.8 [Caenorhabditis elegans] ref|NP_507337.1| ZK218.8 [Caenorhabditis elegans] 3.0E-96
blast CAB05546 Hypothetical protein K08H10.7 [Caenorhabditis elegans] ref|NP_741611.1| RNAi DEfective family member (rde-1) [Caenorhabditis elegans] gb|AAF06159.1| RNA interference promoting factor RDE-1 [Caenorhabditis elegans] 9.0E-33
blast CAB05546 Hypothetical protein K08H10.7 [Caenorhabditis elegans] ref|NP_741611.1| RNAi DEfective family member (rde-1) [Caenorhabditis elegans] gb|AAF06159.1| RNA interference promoting factor RDE-1 [Caenorhabditis elegans] 1.0E-30
blast CAE72296 Hypothetical protein CBG19426 [Caenorhabditis briggsae] 8.0E-29
blast CAE72296 Hypothetical protein CBG19426 [Caenorhabditis briggsae] 9.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.3E-25
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 8.9E-25
Sequence
Target Sequence 
>C. ELEGANS:179064_AT
caagtcccatgtcagttacaaaccccccaccacccgactaggcctctaccccttcgtcag
tgctactggctgtgtgttagttgttgatacgattaagattcatgacattcttttgaaaac
tgttaatttcgaaaaaggtctgcattatgtgaatgccggaactattgaatacatggcttc
ggagtacaattacacgatcaaaataaagaaccactttgtttattcactggcgcctgagct
gagtaagaagaacccaaaagtgaaacaaagcgaaactcctcgattcatcaacccaccatc
tggaaccgtagtcgacaaacttgttgtgtcgggatacaaattcgatttctacctgaattc
tcatcatgcggtgctcggaacctctcgacctgcacattacactgttatgtacgatgatat
gggaatgagtcaggatgaagtttataaaatgactgacgctcttgccttcctctccgccag
atgccgaaaacccatctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGTCCCATGTCAGTTACAAACCC 38 179 18 Antisense
CTTCGTCAGTGCTACTGGCTGTGTG 206 219 69 Antisense
ACATGGCTTCGGAGTACAATTACAC 76 103 188 Antisense
GAACCACTTTGTTTATTCACTGGCG 530 345 225 Antisense
TTCACTGGCGCCTGAGCTGAGTAAG 662 685 240 Antisense
AAGCGAAACTCCTCGATTCATCAAC 356 147 285 Antisense
CCACCATCTGGAACCGTAGTCGACA 389 271 310 Antisense
TCGATTTCTACCTGAATTCTCATCA 50 603 359 Antisense
TTCTCATCATGCGGTGCTCGGAACC 559 691 375 Antisense
TAAAATGACTGACGCTCTTGCCTTC 621 639 462 Antisense
CCGCCAGATGCCGAAAACCCATCTC 424 261 491 Antisense
Sequence Source Affymetrix Proprietary Database