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[179031_s_at]

Description F53F4.7 /REP_DB=WormBase Gene ID /WP=CE10966 /TR=Q20723 /GB=CAB01207.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 71      4grp_total > 40      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 8.1
(-3)
A 		43
(0.4)
A 		70.6
(3)
A 		15.4
(-2.3)
A 		63
B 34.7
(2)
A 		37.6
(3)
A 		20
(-3)
A 		20.6
(-2.8)
A 		18
C 21.9
(-1.6)
A 		10.6
(-3)
A 		47.5
(1.5)
A 		59.3
(3)
A 		49
avr 21.6
(-3) 		30.4
(-0.8) 		46
(3) 		31.8
(-0.5) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1392
1 2 3 4 5 6 range
A 29.4
(-3)
A 		41.3
(-2.9)
A 		605.9
(-0.1)
P 		1240.1
(3)
P 		458.3
(-0.9)
P 		64.5
(-2.8)
A 		1211
B 9.2
(-3)
A 		47.5
(-2.8)
A 		685.7
(0.9)
P 		1041.7
(3)
P 		563.5
(0.2)
P 		75.1
(-2.6)
A 		1033
C 13.3
(-3)
A 		36.3
(-2.9)
A 		406.2
(-1.3)
P 		1400.8
(3)
P 		523.7
(-0.8)
P 		70.1
(-2.8)
M 		1388
avr 17.3
(-3) 		41.7
(-2.9) 		565.9
(-0.3) 		1227.5
(3) 		515.2
(-0.5) 		69.9
(-2.7) 		1210
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 2.9
(-2.7)
A 		9.3
(-0.4)
A 		18.4
(3)
A 		2.2
(-3)
A 		16
B 11.1
(3)
A 		9.3
(1.4)
A 		4.6
(-2.8)
A 		4.4
(-3)
A 		7
C 7.2
(-1.1)
A 		3
(-3)
A 		16.6
(3)
A 		10
(0.1)
A 		14
avr 7.1
(-1.8) 		7.2
(-1.7) 		13.2
(3) 		5.5
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 311
1 2 3 4 5 6 range
A 5.4
(-3)
A 		14.7
(-2.8)
A 		132
(-0.5)
P 		314.5
(3)
P 		138.3
(-0.4)
P 		15.7
(-2.8)
A 		309
B 3.2
(-3)
A 		15.8
(-2.7)
A 		166.5
(0.6)
P 		275.9
(3)
P 		229
(2)
P 		28
(-2.5)
A 		273
C 4.9
(-3)
A 		11.1
(-2.9)
A 		103.4
(-0.9)
P 		281.7
(3)
P 		169.5
(0.6)
P 		23.5
(-2.6)
M 		277
avr 4.5
(-3) 		13.9
(-2.8) 		134
(-0.3) 		290.7
(3) 		178.9
(0.7) 		22.4
(-2.6) 		286
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179031_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14310
Sequence Type Exemplar sequence
Representative Public ID F53F4.7 NCBI
Target Description F53F4.7 /REP_DB=WormBase Gene ID /WP=CE10966 /TR=Q20723 /GB=CAB01207.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073971(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073971 NCBI Caenorhabditis elegans F53F4.7 (F53F4.7) mRNA, complete cds. 11/11 None
SNAP00000012440 ENSEMBL cdna:SNAP chromosome:CEL140:V:13615031:13616286:-1 11/11 None
GENEFINDER00000012454 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13614009:13620315:-1 11/11 None
F53F4.7 ENSEMBL cdna:known chromosome:CEL140:V:13615031:13616559:-1 gene:F53F4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13615030-13616559(-) 96.58 100.0
Public Domain and Genome References
Gene Title
Ensembl F53F4.7
Entrez Gene 179847 Entrez gene
SwissProt Q20723 EMBL-EBI
WormBase CE10966 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01207 Hypothetical protein F53F4.7 [Caenorhabditis elegans] ref|NP_506372.1| F53F4.7 [Caenorhabditis elegans] 0.0
blast CAE66279 Hypothetical protein CBG11524 [Caenorhabditis briggsae] 0.0
blast CAB01206 Hypothetical protein F53F4.8 [Caenorhabditis elegans] ref|NP_506373.1| F53F4.8 [Caenorhabditis elegans] 0.0
blast CAE66278 Hypothetical protein CBG11523 [Caenorhabditis briggsae] 0.0
blast CAE59001 Hypothetical protein CBG02276 [Caenorhabditis briggsae] 0.0
blast CAB01207 Hypothetical protein F53F4.7 [Caenorhabditis elegans] ref|NP_506372.1| F53F4.7 [Caenorhabditis elegans] 1.0E-159
blast CAE66279 Hypothetical protein CBG11524 [Caenorhabditis briggsae] 1.0E-140
blast CAB07694 Hypothetical protein Y48E1B.8 [Caenorhabditis elegans] ref|NP_496853.1| Y48E1B.8 [Caenorhabditis elegans] 4.0E-45
Protein Domains
Database ID Description E-Value
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.7E-117
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR000519 EMBL-EBI P-type trefoil domain 1.4E-6
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506372.1 1 5-24
F53F4.7 1 5-24
GENEFINDER00000012454 2 1133-1155,1176-1195
Sequence
Target Sequence 
>C. ELEGANS:179031_S_AT
aaaccgttttggatgcttcaggctgatacagtttggcgtgataattttcttaacttggtc
gatgtgagtaaatatgagaatagtgatattcttcttgatcaacaaggattcgaaggaacc
gctgatattcgaaaaagaacaatgaacggtgcaaacttctatattccctcaaaatcttcc
agtcagtctttagtagaatcatggttattttggcagaaaagtgtgtatattactgaccca
gatcttgttaaaatgttttgtctccgtggagattattcatgtgattatgttccatacagt
ctggtaacgggatgggaatggatttacggagaccagaaaaacccaccgatcatgattcaa
atggacggagaaactggtggaaataaggagaaagtgcttgaaaaatataatttctggttt
ttggataaaaatgacagatgtaaagcagataaagtttcaaaaggagttcaacatatggtt
gatggaactgtgccacggattatgacgcaatcaaagaatcgcgaacaattttatttgaaa
ctcggagaatttctcaatcaaattccagttttcggacattattccagcattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACCGTTTTGGATGCTTCAGGCTG 496 125 466 Antisense
GCTTCAGGCTGATACAGTTTGGCGT 397 305 480 Antisense
GGATTCGAAGGAACCGCTGATATTC 381 509 571 Antisense
TGCAAACTTCTATATTCCCTCAAAA 628 573 615 Antisense
AAAATCTTCCAGTCAGTCTTTAGTA 312 133 636 Antisense
GTATATTACTGACCCAGATCTTGTT 514 453 690 Antisense
GTTAAAATGTTTTGTCTCCGTGGAG 78 447 712 Antisense
TGTTCCATACAGTCTGGTAACGGGA 677 559 753 Antisense
GAAAAACCCACCGATCATGATTCAA 177 355 801 Antisense
GGAACTGTGCCACGGATTATGACGC 67 531 949 Antisense
GTTTTCGGACATTATTCCAGCATTT 242 449 1033 Antisense
Sequence Source Affymetrix Proprietary Database