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[179013_s_at]

Description T20D3.2 /REP_DB=WormBase Gene ID /WP=CE03671 /TR=O01254 /GB=CAA92485.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 41      4grp_highest_8_8 > 16      4grp_high_8_8 > 60      6grp_highest_nonorm > 06      6grp_total > 41      4grp_total > 59      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7810
1 2 3 4 range
A 10123.3
(1.6)
P 		11968.6
(3)
P 		11657.3
(2.8)
P 		4158.6
(-3)
P 		7810
B 8649.5
(1)
P 		10770.1
(3)
P 		10744.3
(3)
P 		4316.3
(-3)
P 		6454
C 10027
(1.8)
P 		10184.9
(1.9)
P 		11486.2
(3)
P 		4443.1
(-3)
P 		7043
avr 9599.9
(1.5) 		10974.5
(2.7) 		11295.9
(3) 		4306
(-3) 		6990

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9464
1 2 3 4 5 6 range
A 9908.4
(-0.5)
P 		9367
(-0.9)
P 		15445.7
(3)
P 		10070.9
(-0.4)
P 		8160.6
(-1.6)
P 		5981.7
(-3)
P 		9464
B 9369.5
(-0.5)
P 		10997.8
(1.3)
P 		12516
(3)
P 		10341.3
(0.6)
P 		8409.7
(-1.6)
P 		7151.7
(-3)
P 		5364
C 7632.6
(-1.7)
P 		11337.9
(2.1)
P 		10778.3
(1.5)
P 		12198
(3)
P 		7172.9
(-2.1)
P 		6341.3
(-3)
P 		5857
avr 8970.2
(-0.7) 		10567.6
(0.8) 		12913.3
(3) 		10870.1
(1.1) 		7914.4
(-1.7) 		6491.6
(-3) 		6422
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3395
1 2 3 4 range
A 3685.9
(3)
P 		2598.1
(0.9)
P 		3047.4
(1.8)
P 		608.7
(-3)
P 		3077
B 2758.3
(3)
P 		2660
(2.7)
P 		2489.7
(2.1)
P 		919.8
(-3)
P 		1839
C 3319.2
(1.7)
P 		2928.2
(1)
P 		4003.3
(3)
P 		749.2
(-3)
P 		3254
avr 3254.5
(3) 		2728.8
(1.7) 		3180.1
(2.8) 		759.2
(-3) 		2495

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2204
1 2 3 4 5 6 range
A 1826.5
(-1.8)
P 		3331.4
(2.9)
P 		3363.7
(3)
P 		2554.2
(0.5)
P 		2462.5
(0.2)
P 		1456.4
(-3)
P 		1907
B 3260.1
(0.6)
P 		3660.7
(3)
P 		3039.3
(-0.8)
P 		2739.1
(-2.6)
P 		3416.8
(1.5)
P 		2666.9
(-3)
P 		994
C 2829.1
(0.2)
P 		3459.5
(3)
P 		2743.3
(-0.2)
P 		2453
(-1.5)
P 		2321.1
(-2.1)
P 		2122.8
(-3)
P 		1337
avr 2638.6
(-0.6) 		3483.9
(3) 		3048.8
(1.1) 		2582.1
(-0.9) 		2733.5
(-0.2) 		2082
(-3) 		1402
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 179013_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10196
Sequence Type Exemplar sequence
Representative Public ID T20D3.2 NCBI
Target Description T20D3.2 /REP_DB=WormBase Gene ID /WP=CE03671 /TR=O01254 /GB=CAA92485.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069239(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069239 NCBI Caenorhabditis elegans T20D3.2 (T20D3.2) mRNA, complete cds. 11/11 None
SNAP00000040156 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9329090:9330829:-1 11/11 None
GENEFINDER00000040166 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9329090:9330829:-1 11/11 None
T20D3.2.1 ENSEMBL cdna:known chromosome:CEL140:IV:9329034:9330834:-1 gene:T20D3.2 11/11 None
T20D3.2.2 ENSEMBL cdna:known chromosome:CEL140:IV:9329034:9330832:-1 gene:T20D3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9329095-9330835(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D3.2
Entrez Gene 177760 Entrez gene
SwissProt O01254 EMBL-EBI
WormBase CE03671 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92485 Hypothetical protein T20D3.2 [Caenorhabditis elegans] ref|NP_501640.1| T20D3.2 [Caenorhabditis elegans] 1.0E-139
blast CAE74046 Hypothetical protein CBG21698 [Caenorhabditis briggsae] 1.0E-121
Protein Domains
Database ID Description E-Value
Pfam IPR009673 EMBL-EBI Protein of unknown function DUF1261 9.4E-62
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501640.1 1 7-26
T20D3.2.1 1 7-26
T20D3.2.2 1 7-26
SNAP00000040156 1 7-26
GENEFINDER00000040166 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:179013_S_AT
attaatggctttccgtgagactcctcaaaatgctgccatttctgcttgtcaggatattca
attcaattattgtcagacaacgttcaatcagttctttggaatggatgattctatttcttg
gcgcaatggatcttacattttcaatacagttcagaaatatttggaaatgaatgttacaga
acttatcaaggtttgcaacacccgtactcaattctaccaatgcctcggatcttcttatta
tacctgtatgaatctctatactcttctcaacaagccacaacctgatttctcgaacacatt
cgattatgttagaacattccgtggactcgagtggatgtgcgtcggaggattctctgaagt
agtaagccaatgggactgtctcggtactttcccaactacaaatgcgtataactcttgcat
tcaaaacttcaatcaaacagtgtctgctggaaacttctgcccagcagttcaacaaactgg
aagctgtttgaatgacgcctacaatgccgcctgtggagtcggaggagctggatactttgg
atgtgagaacttccgtatcactttcgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTAATGGCTTTCCGTGAGACTCCT 592 11 105 Antisense
TGAGACTCCTCAAAATGCTGCCATT 20 569 120 Antisense
GCTGCCATTTCTGCTTGTCAGGATA 479 297 136 Antisense
TGGATGATTCTATTTCTTGGCGCAA 252 551 206 Antisense
TGCCTCGGATCTTCTTATTATACCT 106 587 325 Antisense
GAATCTCTATACTCTTCTCAACAAG 246 329 354 Antisense
CAACAAGCCACAACCTGATTTCTCG 529 189 372 Antisense
GTGGATGTGCGTCGGAGGATTCTCT 418 489 435 Antisense
CAATGGGACTGTCTCGGTACTTTCC 222 159 472 Antisense
CCTACAATGCCGCCTGTGGAGTCGG 88 269 602 Antisense
TGAGAACTTCCGTATCACTTTCGAC 168 569 648 Antisense
Sequence Source Affymetrix Proprietary Database