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[178969_at]

Description F21A3.7 /REP_DB=WormBase Gene ID /WP=CE20704 /TR=Q9XTE5 /GB=CAB03855.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_highest_8_8 > 39      4grp_high_8_8 > 20      6grp_highest_nonorm > 90      6grp_total > 34      4grp_total > 20      6grp_highest_sub > 01_02_03      6grp_high1_sub > 03      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 218
1 2 3 4 range
A 198.7
(-1.3)
P 		196.9
(-1.3)
P 		152.3
(-3)
P 		312.7
(3)
P 		160
B 239.6
(-0.6)
P 		245.4
(-0.4)
P 		151.5
(-3)
P 		369.1
(3)
P 		218
C 224.6
(-0.7)
P 		196.1
(-1.8)
P 		163.1
(-3)
P 		323.1
(3)
P 		160
avr 221
(-0.8) 		212.8
(-1.1) 		155.6
(-3) 		335
(3) 		179

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 148
1 2 3 4 5 6 range
A 175.1
(-0.3)
P 		245.1
(3)
P 		207.9
(1.3)
P 		175.8
(-0.3)
P 		117.5
(-3)
P 		144.3
(-1.7)
P 		128
B 206.4
(3)
P 		191.1
(2.2)
P 		191
(2.2)
P 		96.9
(-3)
P 		132.5
(-1)
P 		130.1
(-1.2)
P 		110
C 163
(-0.7)
P 		146.4
(-3)
P 		189
(3)
P 		174.3
(0.9)
P 		158.3
(-1.3)
P 		160.5
(-1)
P 		43
avr 181.5
(1.5) 		194.2
(2.8) 		196
(3) 		149
(-1.7) 		136.1
(-3) 		145
(-2.1) 		60
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 72.4
(3)
P 		42.7
(-2.5)
P 		39.8
(-3)
P 		45.8
(-1.9)
P 		33
B 76.4
(2.7)
P 		60.6
(0.5)
P 		35.1
(-3)
P 		78.7
(3)
P 		44
C 74.4
(3)
P 		56.4
(-2.4)
P 		56.9
(-2.3)
P 		54.5
(-3)
P 		20
avr 74.4
(3) 		53.2
(-1.2) 		43.9
(-3) 		59.7
(0.1) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 32.3
(-3)
P 		87.2
(3)
P 		45.3
(-1.6)
P 		44.6
(-1.7)
P 		35.5
(-2.7)
P 		35.1
(-2.7)
P 		55
B 71.8
(3)
P 		63.6
(1.9)
P 		46.4
(-0.3)
P 		25.7
(-3)
P 		53.8
(0.7)
P 		48.5
(-0)
P 		46
C 60.4
(3)
P 		44.7
(-0.7)
P 		48.1
(0.1)
P 		35
(-3)
P 		51.2
(0.8)
P 		53.7
(1.4)
P 		25
avr 54.8
(0.9) 		65.2
(3) 		46.6
(-0.7) 		35.1
(-3) 		46.8
(-0.7) 		45.8
(-0.9) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178969_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12699
Sequence Type Exemplar sequence
Representative Public ID F21A3.7 NCBI
Target Description F21A3.7 /REP_DB=WormBase Gene ID /WP=CE20704 /TR=Q9XTE5 /GB=CAB03855.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074460(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074460 NCBI Caenorhabditis elegans F21A3.7 (F21A3.7) mRNA, complete cds. 11/11 None
SNAP00000044326 ENSEMBL cdna:SNAP chromosome:CEL140:V:15515440:15517795:-1 11/11 None
F21A3.7 ENSEMBL cdna:known chromosome:CEL140:V:15515583:15519928:-1 gene:F21A3.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15515750-15519928(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F21A3.7
Entrez Gene 184752 Entrez gene
SwissProt Q9XTE5 EMBL-EBI
WormBase CE36643 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
6811 ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
45211 postsynaptic membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5230 extracellular ligand-gated ion channel activity inferred from electronic annotation QuickGO AmiGO
30594 neurotransmitter receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03855 Hypothetical protein F21A3.7 [Caenorhabditis elegans] emb|CAB04158.2| Hypothetical protein F21A3.7 [Caenorhabditis elegans] ref|NP_506861.2| F21A3.7 [Caenorhabditis elegans] 0.0
blast CAE71620 Hypothetical protein CBG18584 [Caenorhabditis briggsae] 0.0
blast CAB03855 Hypothetical protein F21A3.7 [Caenorhabditis elegans] emb|CAB04158.2| Hypothetical protein F21A3.7 [Caenorhabditis elegans] ref|NP_506861.2| F21A3.7 [Caenorhabditis elegans] 1.0E-151
blast CAE71620 Hypothetical protein CBG18584 [Caenorhabditis briggsae] 1.0E-117
blast CAE68290 Hypothetical protein CBG13975 [Caenorhabditis briggsae] 5.0E-46
blast CAE68290 Hypothetical protein CBG13975 [Caenorhabditis briggsae] 2.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.3E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506861.2 3 175-193,219-241,418-437
F21A3.7 3 175-193,219-241,418-437
SNAP00000044326 4 4-23,36-54,80-102,279-298
Sequence
Target Sequence 
>C. ELEGANS:178969_AT
gatcgttttctggtattccgttgattggactctactatttcatattattagccatcatcg
gaatgtcgacagtcaccacgtcaatgttcgtgtacttggaacgagagtcgcgggtcaaaa
tgaatgttccgtggtatttgaagtggctctcatttgacatatcgcccagtaaaattgcta
ggaaactatcccgttatcgtccacaggcgatgagccaacctccgtcggagcatctcctgt
ccaatggacatgttcgaagtggaatccgtgataaggctcgaggagtcgccgcgtcacttc
tatcgctgctgccacgaccgggcagtggagttggaaacggaggacccggcggcgacaatg
gcaacagtccattccacatgcaacaccttcacgcttaccaccaggacaaccctactcctg
ctcgggagtcgatacgaagacggctgagcatgtcccagtatgataacatgctgatttatc
aatattacgaacaagctctcgaggatatcgccaacggggtctctagaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCGTTTTCTGGTATTCCGTTGAT 587 417 638 Antisense
GAATGTCGACAGTCACCACGTCAAT 648 331 698 Antisense
CTCTCATTTGACATATCGCCCAGTA 372 229 784 Antisense
TAGGAAACTATCCCGTTATCGTCCA 648 651 816 Antisense
TCGTCCACAGGCGATGAGCCAACCT 315 603 834 Antisense
CTGCTGCCACGACCGGGCAGTGGAG 506 237 943 Antisense
CCGGCGGCGACAATGGCAACAGTCC 420 263 983 Antisense
CCTACTCCTGCTCGGGAGTCGATAC 202 267 1048 Antisense
ACGAAGACGGCTGAGCATGTCCCAG 696 91 1071 Antisense
GAACAAGCTCTCGAGGATATCGCCA 288 353 1126 Antisense
ATATCGCCAACGGGGTCTCTAGAAT 414 11 1142 Antisense
Sequence Source Affymetrix Proprietary Database