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[178960_at]

Description F35E8.6 /REP_DB=WormBase Gene ID /WP=CE15956 /TR=O45452 /GB=CAB04295.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 66      4grp_total > 31      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 106.4
(3)
A 		71.8
(0.8)
A 		11.5
(-3)
A 		78.6
(1.2)
A 		95
B 100.2
(3)
A 		8.4
(-3)
A 		47.8
(-0.4)
A 		85.7
(2.1)
A 		92
C 77.6
(3)
A 		14.1
(-2.8)
A 		12.2
(-3)
A 		40.3
(-0.4)
A 		65
avr 94.7
(3) 		31.4
(-2.4) 		23.8
(-3) 		68.2
(0.8) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 8
(-2.9)
A 		6.2
(-3)
A 		28.3
(-2.2)
A 		37.3
(-1.8)
A 		7.1
(-3)
A 		165.9
(3)
A 		160
B 9.2
(-2.9)
A 		7.9
(-3)
A 		59.4
(1.2)
A 		12.2
(-2.7)
A 		22.6
(-1.8)
A 		81.8
(3)
A 		74
C 6.4
(-2.9)
A 		5.3
(-3)
A 		12.1
(-2.4)
A 		14.1
(-2.2)
A 		5.7
(-3)
A 		73.5
(3)
A 		68
avr 7.9
(-2.9) 		6.5
(-3) 		33.3
(-1.4) 		21.2
(-2.1) 		11.8
(-2.7) 		107.1
(3) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 38.7
(3)
A 		15.6
(-0.9)
A 		3
(-3)
A 		11.5
(-1.6)
A 		36
B 32
(3)
A 		2.1
(-3)
A 		11.1
(-1.2)
A 		18.3
(0.3)
A 		30
C 25.7
(3)
A 		4.1
(-3)
A 		4.3
(-2.9)
A 		6.8
(-2.3)
A 		22
avr 32.1
(3) 		7.3
(-2.7) 		6.1
(-3) 		12.2
(-1.6) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 1.5
(-3)
A 		2.2
(-2.9)
A 		6.2
(-2.3)
A 		9.5
(-1.8)
A 		2.1
(-2.9)
A 		40.4
(3)
A 		39
B 3.2
(-2.9)
A 		2.6
(-3)
A 		14.4
(-0.5)
A 		3.2
(-2.9)
A 		9.2
(-1.6)
A 		30.5
(3)
A 		28
C 2.4
(-2.8)
A 		1.6
(-3)
A 		3.1
(-2.6)
A 		2.8
(-2.7)
A 		1.8
(-2.9)
A 		24.6
(3)
A 		23
avr 2.4
(-2.9) 		2.1
(-3) 		7.9
(-1.8) 		5.2
(-2.4) 		4.4
(-2.5) 		31.8
(3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178960_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13328
Sequence Type Exemplar sequence
Representative Public ID F35E8.6 NCBI
Target Description F35E8.6 /REP_DB=WormBase Gene ID /WP=CE15956 /TR=O45452 /GB=CAB04295.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074580(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074580 NCBI Caenorhabditis elegans F35E8.6 (F35E8.6) mRNA, complete cds. 11/11 None
SNAP00000019512 ENSEMBL cdna:SNAP chromosome:CEL140:V:15914735:15915640:1 11/11 None
GENEFINDER00000019525 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15914735:15915640:1 11/11 None
F35E8.6 ENSEMBL cdna:known chromosome:CEL140:V:15914732:15915640:1 gene:F35E8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15914731-15915640(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E8.6
Entrez Gene 185297 Entrez gene
SwissProt O45452 EMBL-EBI
WormBase CE15956 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04295 Hypothetical protein F35E8.6 [Caenorhabditis elegans] ref|NP_506981.1| F35E8.6 [Caenorhabditis elegans] 1.0E-120
blast CAB04295 Hypothetical protein F35E8.6 [Caenorhabditis elegans] ref|NP_506981.1| F35E8.6 [Caenorhabditis elegans] 1.0E-119
blast CAB04296 Hypothetical protein F35E8.7 [Caenorhabditis elegans] ref|NP_506982.1| F35E8.7 [Caenorhabditis elegans] 4.0E-68
blast CAE65459 Hypothetical protein CBG10424 [Caenorhabditis briggsae] 2.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.6E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.4E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.1E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.6E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.4E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.1E-11
Sequence
Target Sequence 
>C. ELEGANS:178960_AT
gacaatgcgagacccttcatttgttatagaacgggaaatgctgcgggagcggttatggat
gatatcaaggcagatgccattagcatctgtccgaaatcttgcggatattgctgtgagact
agtgcatacaattgcccaaacgctgctttaccaagcatcgattgctcaaaagtaacacct
acacagtgcaaaagcccagcgtggagaccagtcattgctgcagaatgcccttctgcatgt
ggtttctgcagcgaaaatggatgcattgacgcagtgaaggaatgtgctaacgatataacc
atttgtaacgctgttaattggcaagatttcgtcaacaagaactgccaaaagacttgcaag
agatgccccgccaccaccgcagctccagctgcaggtgtcacaaccactgcagctccagct
cccgttccagtcgtcccaactgcagctccaccagcagttgttaccaccgctgcaccagcc
acctgcacatcgtacatgcctgacagcaccacaaagtgtgcggaatacgctcgtaatgga
ttctgtacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAATGCGAGACCCTTCATTTGTT 44 365 220 Antisense
GCCATTAGCATCTGTCCGAAATCTT 365 281 295 Antisense
TACAATTGCCCAAACGCTGCTTTAC 92 643 346 Antisense
CCTACACAGTGCAAAAGCCCAGCGT 493 267 397 Antisense
CCCAGCGTGGAGACCAGTCATTGCT 215 257 414 Antisense
AGTCATTGCTGCAGAATGCCCTTCT 551 61 429 Antisense
GAATGCCCTTCTGCATGTGGTTTCT 140 331 442 Antisense
AAAGACTTGCAAGAGATGCCCCGCC 229 123 567 Antisense
CAGCTGCAGGTGTCACAACCACTGC 530 203 605 Antisense
GCACATCGTACATGCCTGACAGCAC 124 317 704 Antisense
ATACGCTCGTAATGGATTCTGTACC 319 23 744 Antisense
Sequence Source Affymetrix Proprietary Database