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[178946_at]

Description F21A3.5 /REP_DB=WormBase Gene ID /WP=CE09519 /TR=O17828 /GB=CAB04156.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 55      4grp_total > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 1.7
(-3)
A 		2.4
(-2.9)
A 		2.7
(-2.9)
A 		57.7
(3)
A 		56
B 30.7
(3)
A 		3.5
(-2.7)
A 		1.9
(-3)
A 		25.2
(1.9)
A 		29
C 2.9
(-2.7)
A 		1.8
(-3)
A 		21.7
(3)
A 		3.9
(-2.4)
A 		20
avr 11.8
(-0.9) 		2.6
(-3) 		8.8
(-1.6) 		28.9
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 2.8
(-2.6)
A 		2.8
(-2.6)
A 		15.4
(2)
A 		1.6
(-3)
A 		18.1
(3)
A 		4.9
(-1.8)
A 		17
B 19
(-1)
A 		2.9
(-2.9)
A 		29.2
(0.2)
A 		2
(-3)
A 		23.8
(-0.5)
A 		53.7
(3)
A 		52
C 1.1
(-3)
A 		29.3
(1.2)
A 		41.5
(3)
A 		6.9
(-2.1)
A 		23.1
(0.3)
A 		27.4
(0.9)
A 		40
avr 7.6
(-2) 		11.7
(-1) 		28.7
(3) 		3.5
(-3) 		21.7
(1.3) 		28.7
(3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 0.6
(-2.9)
A 		0.5
(-3)
A 		0.7
(-2.8)
A 		8.4
(3)
A 		8
B 9.8
(3)
A 		0.9
(-2.7)
A 		0.4
(-3)
A 		5.4
(0.2)
A 		9
C 0.9
(-2.7)
A 		0.5
(-3)
A 		7.6
(3)
A 		0.7
(-2.8)
A 		7
avr 3.8
(1.6) 		0.6
(-3) 		2.9
(0.3) 		4.8
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 0.5
(-2.9)
A 		1
(-2.3)
A 		3.4
(0.5)
A 		0.4
(-3)
A 		5.5
(3)
A 		1.2
(-2.1)
A 		5
B 6.6
(-1.1)
A 		1
(-2.8)
A 		7.1
(-1)
A 		0.5
(-3)
A 		9.7
(-0.2)
A 		20
(3)
A 		20
C 0.4
(-3)
A 		8.9
(2)
A 		10.6
(3)
A 		1.4
(-2.4)
A 		7.5
(1.2)
A 		9.2
(2.2)
A 		10
avr 2.5
(-1.9) 		3.6
(-1.2) 		7
(1) 		0.8
(-3) 		7.6
(1.4) 		10.1
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178946_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13466
Sequence Type Exemplar sequence
Representative Public ID F21A3.5 NCBI
Target Description F21A3.5 /REP_DB=WormBase Gene ID /WP=CE09519 /TR=O17828 /GB=CAB04156.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074465(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074465 NCBI Caenorhabditis elegans F21A3.5 (F21A3.5) mRNA, complete cds. 11/11 None
SNAP00000007994 ENSEMBL cdna:SNAP chromosome:CEL140:V:15540842:15545499:1 9/11 None
GENEFINDER00000007995 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15540842:15545385:1 11/11 None
F21A3.5 ENSEMBL cdna:known chromosome:CEL140:V:15540842:15545385:1 gene:F21A3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15540841-15545385(+) 95.31 100.0
Public Domain and Genome References
Gene Title
Ensembl F21A3.5
Entrez Gene 184750 Entrez gene
SwissProt O17828 EMBL-EBI
WormBase CE09519 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4289 subtilase activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04156 Hypothetical protein F21A3.5 [Caenorhabditis elegans] ref|NP_506866.1| F21A3.5 [Caenorhabditis elegans] 0.0
blast CAE71636 Hypothetical protein CBG18603 [Caenorhabditis briggsae] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR006674 EMBL-EBI Metal-dependent phosphohydrolase, HD subdomain 5.1E-5
Sequence
Target Sequence 
>C. ELEGANS:178946_AT
gtccaaagggtccacgtgctggagcacaaatttcgactgtgaattatcgtggaatcattg
acgatttctacaaaattgtgtgcagcggacgaccggcttgggctcttcattggcgggatg
taatgctcgattgggaccggaaactggagaatacgccagaaacatcgaaaatgttcgaag
cctacgaaaaacaccagagatctctcgaaatttcggaagaatgggaacgtctacaagcga
ctcgcgaacattatacagtgatgaaaaatcacacagaaacggaaatggcaaagaatcagg
cggcaattgttgagtatttgatgccagaagaagaagctaaagaggaacctattgataaga
agaaagatccagaagaagaagcagcagcagctgttgtagggaagaaaagaagaggaagaa
agcctaaaaagaaggatgatccgaaaatggaattgaaggatgaagtgaaagatttgaaag
atttcgtggttgaaggtatacaagttacagaattttcaaaaagtcggcttcatatgagcg
gcaaacgattttttccaacaaattgccggaattggaattgccgatttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCCAAAGGGTCCACGTGCTGGAGC 382 473 986 Antisense
AATTGTGTGCAGCGGACGACCGGCT 378 179 1059 Antisense
GGGCTCTTCATTGGCGGGATGTAAT 412 493 1085 Antisense
GATGTAATGCTCGATTGGGACCGGA 319 409 1102 Antisense
GAAGCCTACGAAAAACACCAGAGAT 413 345 1162 Antisense
ATGGGAACGTCTACAAGCGACTCGC 329 49 1206 Antisense
CAAGCGACTCGCGAACATTATACAG 16 187 1219 Antisense
GAATCAGGCGGCAATTGTTGAGTAT 621 327 1278 Antisense
CAGCAGCAGCTGTTGTAGGGAAGAA 559 203 1367 Antisense
GTCGGCTTCATATGAGCGGCAAACG 151 471 1508 Antisense
GCCGGAATTGGAATTGCCGATTTGC 658 283 1550 Antisense
Sequence Source Affymetrix Proprietary Database