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[178944_at]

Description F45D3.2 /REP_DB=WormBase Gene ID /WP=CE10470 /TR=Q20422 /GB=CAB01503.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 98      6grp_total > 67      4grp_total > 01      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5732
1 2 3 4 range
A 783
(-2.9)
P 		715
(-3)
P 		1236.4
(-2.4)
P 		5931
(3)
P 		5216
B 789.7
(-3)
P 		923.2
(-2.8)
P 		1194.8
(-2.5)
P 		5265.8
(3)
P 		4476
C 893.1
(-3)
P 		967.6
(-2.9)
P 		1253
(-2.6)
P 		6447
(3)
P 		5554
avr 821.9
(-3) 		868.6
(-2.9) 		1228.1
(-2.5) 		5881.3
(3) 		5059

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1749
1 2 3 4 5 6 range
A 868.1
(-2.1)
P 		664.3
(-2.9)
P 		741.3
(-2.6)
P 		923
(-1.8)
P 		645
(-3)
P 		2092.8
(3)
P 		1448
B 796.3
(-2.2)
P 		554.5
(-3)
P 		788
(-2.2)
P 		870.6
(-1.9)
P 		581.7
(-2.9)
P 		2303.8
(3)
P 		1749
C 767.9
(-2.2)
P 		567.3
(-3)
P 		931.9
(-1.6)
P 		816.6
(-2.1)
P 		632.7
(-2.8)
P 		2151.6
(3)
P 		1584
avr 810.8
(-2.2) 		595.4
(-3) 		820.4
(-2.1) 		870.1
(-2) 		619.8
(-2.9) 		2182.7
(3) 		1587
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 967
1 2 3 4 range
A 285.1
(-1.9)
P 		155.2
(-3)
P 		323.2
(-1.6)
P 		868.2
(3)
P 		713
B 251.8
(-2.8)
P 		228
(-3)
P 		276.9
(-2.7)
P 		1122.2
(3)
P 		894
C 295.6
(-2.9)
P 		278.2
(-3)
P 		436.7
(-1.8)
P 		1087.1
(3)
P 		809
avr 277.5
(-2.6) 		220.5
(-3) 		345.6
(-2.1) 		1025.8
(3) 		805

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 699
1 2 3 4 5 6 range
A 160
(-3)
P 		236.2
(-1.7)
P 		161.4
(-3)
P 		234.1
(-1.7)
P 		194.6
(-2.4)
P 		509.5
(3)
P 		350
B 277.1
(-2.2)
P 		184.6
(-3)
P 		191.3
(-2.9)
P 		230.6
(-2.6)
P 		236.3
(-2.5)
P 		859.1
(3)
P 		675
C 284.6
(-1.7)
P 		173.1
(-2.9)
P 		237.2
(-2.2)
P 		164.2
(-3)
P 		204.7
(-2.6)
P 		720.3
(3)
P 		556
avr 240.6
(-2.5) 		198
(-3) 		196.6
(-3) 		209.6
(-2.8) 		211.9
(-2.8) 		696.3
(3) 		500
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178944_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13582
Sequence Type Exemplar sequence
Representative Public ID F45D3.2 NCBI
Target Description F45D3.2 /REP_DB=WormBase Gene ID /WP=CE10470 /TR=Q20422 /GB=CAB01503.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073740(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073740 NCBI Caenorhabditis elegans F45D3.2 (F45D3.2) mRNA, complete cds. 10/11 None
SNAP00000028859 ENSEMBL cdna:SNAP chromosome:CEL140:V:12543666:12545055:1 11/11 None
GENEFINDER00000028866 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12543666:12545055:1 11/11 None
F45D3.2.2 ENSEMBL cdna:known chromosome:CEL140:V:12543627:12545055:1 gene:F45D3.2 11/11 None
F45D3.2.1 ENSEMBL cdna:known chromosome:CEL140:V:12543628:12545562:1 gene:F45D3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12543665-12545055(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45D3.2
Entrez Gene 179716 Entrez gene
SwissProt Q20422 EMBL-EBI
WormBase CE38517 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE75509 Hypothetical protein CBG23522 [Caenorhabditis briggsae] 1.0E-132
blast CAB01503 Hypothetical protein F45D3.2 [Caenorhabditis elegans] ref|NP_506141.2| F45D3.2 [Caenorhabditis elegans] 1.0E-125
blast CAB01503 Hypothetical protein F45D3.2 [Caenorhabditis elegans] ref|NP_506141.2| F45D3.2 [Caenorhabditis elegans] 1.0E-120
blast CAE75509 Hypothetical protein CBG23522 [Caenorhabditis briggsae] 1.0E-99
Sequence
Target Sequence 
>C. ELEGANS:178944_AT
ggattagcagctgatggacgcccaagagcattttcaagcgctgccactttgcaacacgga
aaagatcaagagaaacgacgaaagagaacaagaaagatggaaccatcacttccaattaca
acaagtaccattacatgttcctcgaattcgtctccctcatcaagctctataactacatct
gtgctccgaacattagtagaagatgatgatgcagatgtgcatccaaataaagttcgacca
attcaagtaacaaaagaggaagaggatcttgttagaagaaaaactcaagaattgctcaaa
tccatgccccttcaaaaattagagccagaagtcagcgcaagtggcccacttcgccgtctt
attcgagacgaagaccttccagacgaggtcggaccagattcgccattccgccactaccca
aagtatcagcagaagccccgttcacaactagtcatgcttcaaggaatccaagcggcagtc
gcatgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATTAGCAGCTGATGGACGCCCAA 568 507 382 Antisense
TGGACGCCCAAGAGCATTTTCAAGC 676 545 396 Antisense
TTTCAAGCGCTGCCACTTTGCAACA 442 665 413 Antisense
GTACCATTACATGTTCCTCGAATTC 471 457 506 Antisense
TCTCCCTCATCAAGCTCTATAACTA 382 611 532 Antisense
TAACTACATCTGTGCTCCGAACATT 120 639 551 Antisense
CAAGAATTGCTCAAATCCATGCCCC 695 183 667 Antisense
GACGAGGTCGGACCAGATTCGCCAT 249 377 763 Antisense
CCGCCACTACCCAAAGTATCAGCAG 88 263 789 Antisense
CCGTTCACAACTAGTCATGCTTCAA 158 265 819 Antisense
GGAATCCAAGCGGCAGTCGCATGTA 219 533 844 Antisense
Sequence Source Affymetrix Proprietary Database