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[178936_at]

Description F21G4.3 /REP_DB=WormBase Gene ID /WP=CE09549 /TR=Q93551 /GB=CAB02666.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 04      4grp_total > 40      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 3.4
(-3)
A 		49
(1.2)
A 		67.8
(3)
A 		45.4
(0.9)
A 		64
B 21.5
(-2.3)
A 		62.2
(2)
A 		71.6
(3)
A 		14.7
(-3)
A 		57
C 12
(-2.4)
A 		5.5
(-3)
A 		76.1
(3)
A 		36.3
(-0.4)
A 		71
avr 12.3
(-3) 		38.9
(-0.3) 		71.8
(3) 		32.1
(-1) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 80.3
(3)
A 		4
(-3)
A 		42.7
(0)
A 		4.1
(-3)
A 		6.6
(-2.8)
A 		4.2
(-3)
A 		76
B 14.7
(-0.6)
A 		33.5
(3)
A 		4
(-2.7)
A 		31
(2.5)
A 		16.7
(-0.2)
A 		2.3
(-3)
A 		31
C 1.4
(-3)
A 		33.8
(2.8)
A 		4.6
(-2.4)
A 		34.8
(3)
A 		29.6
(2.1)
A 		4.2
(-2.5)
A 		33
avr 32.1
(3) 		23.8
(1.3) 		17.1
(-0.2) 		23.3
(1.1) 		17.6
(-0.1) 		3.6
(-3) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 1.2
(-3)
A 		10.6
(0.4)
A 		17.7
(3)
A 		6.6
(-1)
A 		17
B 6.9
(-1.3)
A 		15.4
(2.5)
A 		16.6
(3)
A 		3.1
(-3)
A 		14
C 4
(-2.4)
A 		1.6
(-3)
A 		26.5
(3)
A 		6.1
(-1.9)
A 		25
avr 4
(-3) 		9.2
(-1.1) 		20.3
(3) 		5.3
(-2.5) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 14.8
(3)
A 		1.4
(-2.8)
A 		9.3
(0.6)
A 		1.1
(-3)
A 		2
(-2.6)
A 		1
(-3)
A 		14
B 5.1
(-0.5)
A 		11.1
(3)
A 		1
(-2.9)
A 		8.2
(1.3)
A 		6.8
(0.5)
A 		0.9
(-3)
A 		10
C 0.5
(-3)
A 		10.3
(3)
A 		1.2
(-2.6)
A 		7
(1)
A 		9.6
(2.6)
A 		1.4
(-2.4)
A 		10
avr 6.8
(2.3) 		7.6
(3) 		3.8
(-0.5) 		5.4
(1) 		6.1
(1.6) 		1.1
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178936_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16745
Sequence Type Exemplar sequence
Representative Public ID F21G4.3 NCBI
Target Description F21G4.3 /REP_DB=WormBase Gene ID /WP=CE09549 /TR=Q93551 /GB=CAB02666.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077259(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077259 NCBI Caenorhabditis elegans F21G4.3 (F21G4.3) mRNA, complete cds. 11/11 None
GENEFINDER00000036638 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9895074:9897586:1 11/11 None
SNAP00000036631 ENSEMBL cdna:SNAP chromosome:CEL140:X:9895482:9896987:1 10/11 None
F21G4.3 ENSEMBL cdna:known chromosome:CEL140:X:9895482:9896721:1 gene:F21G4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9895481-9896721(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F21G4.3
Entrez Gene 184792 Entrez gene
SwissProt Q93551 EMBL-EBI
WormBase CE09549 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE58732 Hypothetical protein CBG01918 [Caenorhabditis briggsae] 1.0E-150
blast CAB02666 Hypothetical protein F21G4.3 [Caenorhabditis elegans] ref|NP_509660.1| F21G4.3 [Caenorhabditis elegans] 1.0E-116
blast CAE58732 Hypothetical protein CBG01918 [Caenorhabditis briggsae] 1.0E-111
blast CAB02666 Hypothetical protein F21G4.3 [Caenorhabditis elegans] ref|NP_509660.1| F21G4.3 [Caenorhabditis elegans] 1.0E-109
blast CAE58732 Hypothetical protein CBG01918 [Caenorhabditis briggsae] 1.0E-95
blast CAB02666 Hypothetical protein F21G4.3 [Caenorhabditis elegans] ref|NP_509660.1| F21G4.3 [Caenorhabditis elegans] 2.0E-89
blast CAB00871 Hypothetical protein R09H10.2 [Caenorhabditis elegans] ref|NP_501961.1| R09H10.2 [Caenorhabditis elegans] 5.0E-28
blast AAA62296 Hypothetical protein C28H8.8 [Caenorhabditis elegans] 1.0E-23
Protein Domains
Database ID Description E-Value
Pfam IPR002485 EMBL-EBI Protein of unknown function DUF13 2.0E-40
Pfam IPR002485 EMBL-EBI Protein of unknown function DUF13 2.0E-40
Pfam IPR002485 EMBL-EBI Protein of unknown function DUF13 2.5E-19
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000036638 1 12-29
Sequence
Target Sequence 
>C. ELEGANS:178936_AT
tgtggcacaagttaggatcctacacagacaaatgtcgttcatcaactgatttgtggcata
tggaacctgaaatagtttctggaacagctttgaaatttttcactccaaatccaacagaca
catcagccacatttgtaagggttggaattggtgcaaatgtttctattgaacagtatttca
agaaaaaattgtcagcggattctgagttttttggggcagatccggctttcatggaaaatt
cgcaaatttattcatcaatcgggacgttcttcccgtttgcggttggaaatgagactggac
taaaacaagcaacacttaaattctcagtcgaacgaatgtcttcgaagtttgtcaaccaca
ttgggattgacattttcttcaaaaatctcattcgaaaggatataattgattacttaattt
ttgaaggcgacggtggagaatacgacatgatgtctcatcttgctaggaacggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGGCACAAGTTAGGATCCTACAC 326 555 119 Antisense
GACAAATGTCGTTCATCAACTGATT 680 365 145 Antisense
GAAATTTTTCACTCCAAATCCAACA 355 361 210 Antisense
TCCAACAGACACATCAGCCACATTT 8 589 228 Antisense
AGCGGATTCTGAGTTTTTTGGGGCA 442 79 312 Antisense
TTTTTTGGGGCAGATCCGGCTTTCA 133 187 325 Antisense
TATTCATCAATCGGGACGTTCTTCC 639 661 367 Antisense
TTCTTCCCGTTTGCGGTTGGAAATG 36 675 385 Antisense
GCAACACTTAAATTCTCAGTCGAAC 478 319 427 Antisense
ATGTCTTCGAAGTTTGTCAACCACA 246 47 454 Antisense
GATGTCTCATCTTGCTAGGAACGGT 320 409 567 Antisense
Sequence Source Affymetrix Proprietary Database