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[178931_s_at]

Description T20D3.8 /REP_DB=WormBase Gene ID /WP=CE23990 /TR=O01259 /GB=CAA92490.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 13      4grp_high_8_8 > 30      6grp_highest_nonorm > 95      6grp_total > 97      4grp_total > 24      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 388
1 2 3 4 range
A 490.7
(-3)
P 		535.1
(-2)
P 		703.4
(1.9)
P 		748.8
(3)
P 		258
B 461.6
(-3)
P 		679
(0.7)
P 		629.3
(-0.2)
P 		814.7
(3)
P 		353
C 509.6
(-3)
P 		667.7
(-0.2)
P 		624.3
(-1)
P 		849.3
(3)
P 		340
avr 487.3
(-3) 		627.3
(-0.4) 		652.3
(0.1) 		804.3
(3) 		317

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 639
1 2 3 4 5 6 range
A 740.2
(-1.8)
P 		635.2
(-3)
P 		740.8
(-1.8)
P 		856.2
(-0.4)
P 		953.4
(0.8)
P 		1143.7
(3)
P 		509
B 591.9
(-2.1)
P 		518.1
(-3)
P 		535.6
(-2.8)
P 		776.9
(0.3)
P 		854.1
(1.2)
P 		994
(3)
P 		476
C 651.4
(-2)
P 		554
(-3)
P 		699.3
(-1.6)
P 		628.1
(-2.3)
P 		844.5
(-0.1)
P 		1157.5
(3)
P 		604
avr 661.2
(-2) 		569.1
(-3) 		658.6
(-2) 		753.7
(-0.9) 		884
(0.6) 		1098.4
(3) 		529
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 178.7
(2.6)
P 		116.2
(-2.5)
P 		183.9
(3)
P 		109.6
(-3)
P 		74
B 147.2
(-2.7)
P 		167.7
(1.7)
P 		145.8
(-3)
P 		173.6
(3)
P 		28
C 168.7
(-0.9)
P 		192
(0.9)
P 		217.6
(3)
P 		143.2
(-3)
P 		74
avr 164.9
(0.4) 		158.6
(-0.5) 		182.4
(3) 		142.1
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 261
1 2 3 4 5 6 range
A 136.5
(-3)
P 		225.9
(0.5)
P 		161.3
(-2)
P 		217.2
(0.2)
P 		287.7
(3)
P 		278.5
(2.6)
P 		151
B 206
(-1.1)
P 		172.4
(-1.9)
P 		130.1
(-3)
P 		205.8
(-1.1)
P 		347
(2.4)
P 		370.7
(3)
P 		241
C 241.4
(-0.4)
P 		169
(-2)
P 		178
(-1.8)
P 		126.3
(-3)
P 		273.3
(0.4)
P 		387.5
(3)
P 		261
avr 194.6
(-1.8) 		189.1
(-2) 		156.5
(-3) 		183.1
(-2.2) 		302.7
(1.6) 		345.6
(3) 		189
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178931_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9549
Sequence Type Exemplar sequence
Representative Public ID T20D3.8 NCBI
Target Description T20D3.8 /REP_DB=WormBase Gene ID /WP=CE23990 /TR=O01259 /GB=CAA92490.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069244(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069244 NCBI Caenorhabditis elegans T20D3.8 (T20D3.8) mRNA, complete cds. 11/11 None
GENEFINDER00000040165 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9339778:9343111:-1 11/11 None
T20D3.8 ENSEMBL cdna:known chromosome:CEL140:IV:9341970:9343111:-1 gene:T20D3.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9341975-9343117(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D3.8
Entrez Gene 177765 Entrez gene
SwissProt O01259 EMBL-EBI
WormBase CE23990 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:267416_AT phosphatidylinositolglycan synthase family protein at
CANINE:1604240_AT similar to Phosphatidylinositol N-acetylglucosaminyltransferase subunit C (Phosphatidylinositol-glycan biosynthesis, class C protein) (PIG-C) cfa
CANINE:1583185_AT similar to Phosphatidylinositol N-acetylglucosaminyltransferase subunit C (Phosphatidylinositol-glycan biosynthesis, class C protein) (PIG-C) cfa
CANINE_2:CFA.1739.1.A1_AT similar to Phosphatidylinositol N-acetylglucosaminyltransferase subunit C (Phosphatidylinositol-glycan biosynthesis, class C protein) (PIG-C) cfa
CANINE_2:CFA.20968.1.S1_S_AT similar to Phosphatidylinositol N-acetylglucosaminyltransferase subunit C (Phosphatidylinositol-glycan biosynthesis, class C protein) (PIG-C) cfa
CANINE_2:CFAAFFX.22647.1.S1_AT similar to Phosphatidylinositol N-acetylglucosaminyltransferase subunit C (Phosphatidylinositol-glycan biosynthesis, class C protein) (PIG-C) cfa
CANINE_2:CFAAFFX.22647.1.S1_S_AT similar to Phosphatidylinositol N-acetylglucosaminyltransferase subunit C (Phosphatidylinositol-glycan biosynthesis, class C protein) (PIG-C) cfa
DROSOPHILA_2:1637783_AT dm
DROSGENOME1:148104_AT dm
CHICKEN:GGA.7276.1.S1_AT phosphatidylinositol glycan, class C gga
HG-U133_PLUS_2:215242_AT phosphatidylinositol glycan, class C hs
HG-U133A_2:215242_AT phosphatidylinositol glycan, class C hs
HG-U133A:215242_AT phosphatidylinositol glycan, class C hs
HG-U95AV2:751_AT phosphatidylinositol glycan, class C hs
HC-G110:751_AT phosphatidylinositol glycan, class C hs
HG-U95AV2:36696_AT phosphatidylinositol glycan, class C hs
HG-U133_PLUS_2:202846_S_AT phosphatidylinositol glycan, class C hs
HG-U133A:202846_S_AT phosphatidylinositol glycan, class C hs
HG-FOCUS:202846_S_AT phosphatidylinositol glycan, class C hs
HG-U133A_2:202846_S_AT phosphatidylinositol glycan, class C hs
HU35KSUBB:RC_N38882_AT phosphatidylinositol glycan, class C hs
HU35KSUBD:RC_AA435564_AT phosphatidylinositol glycan, class C hs
HUGENEFL:D85418_AT phosphatidylinositol glycan, class C hs
U133_X3P:G4505794_3P_A_AT phosphatidylinositol glycan, class C hs
U133_X3P:HS.97375.0.S1_3P_AT phosphatidylinositol glycan, class C hs
U133_X3P:HS.306173.0.S1_3P_S_AT phosphatidylinositol glycan, class C hs
HG-U133A_2:216593_S_AT phosphatidylinositol glycan, class C hs
HG-U133_PLUS_2:216593_S_AT phosphatidylinositol glycan, class C hs
HG-U133A:216593_S_AT phosphatidylinositol glycan, class C hs
HG-U95AV2:36697_G_AT phosphatidylinositol glycan, class C hs
HG-U95AV2:37080_AT phosphatidylinositol glycan, class C hs
HG-U95D:74850_AT phosphatidylinositol glycan, class C hs
HU35KSUBB:RC_AA180453_AT Phosphatidylinositol glycan, class C hs
MG-U74BV2:113737_AT phosphatidylinositol glycan, classC mm
MG-U74BV2:163000_AT phosphatidylinositol glycan, classC mm
MOE430A:1424189_AT phosphatidylinositol glycan, classC mm
MOUSE430A_2:1424189_AT phosphatidylinositol glycan, classC mm
MOUSE430_2:1424189_AT phosphatidylinositol glycan, classC mm
MOE430A:1424190_AT phosphatidylinositol glycan, classC mm
MOUSE430A_2:1424190_AT phosphatidylinositol glycan, classC mm
MOUSE430_2:1424190_AT phosphatidylinositol glycan, classC mm
MU19KSUBC:TC35521_AT Phosphatidylinositol glycan, classC (Pigc), mRNA mm
MU19KSUBC:TC38399_AT Phosphatidylinositol glycan, classC (Pigc), mRNA mm
Protein Similarities
Method ID Description E-Value
blast CAA92489 Hypothetical protein T20D3.7 [Caenorhabditis elegans] ref|NP_501644.1| related to yeast Vacuolar Protein Sorting factor family member (vps-26) [Caenorhabditis elegans] 0.0
blast O01258 Vacuolar protein sorting 26 homolog (VPS26 protein homolog) 0.0
blast CAE74042 Hypothetical protein CBG21693 [Caenorhabditis briggsae] 0.0
blast CAA92490 Hypothetical protein T20D3.8 [Caenorhabditis elegans] ref|NP_501645.1| T20D3.8 [Caenorhabditis elegans] 1.0E-137
blast CAE74041 Hypothetical protein CBG21692 [Caenorhabditis briggsae] 1.0E-104
blast XP_537194 PREDICTED: similar to Phosphatidylinositol N-acetylglucosaminyltransferase subunit C (Phosphatidylinositol-glycan biosynthesis, class C protein) (PIG-C) [Canis familiaris] 1.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR009450 EMBL-EBI Phosphatidylinositol N-acetylglucosaminyltransferase 6.2E-111
Pfam IPR005377 EMBL-EBI Vacuolar protein sorting-associated protein 26 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501645.1 6 45-67,77-99,112-131,186-203,210-232,237-259
T20D3.8 6 45-67,77-99,112-131,186-203,210-232,237-259
GENEFINDER00000040165 6 45-67,77-99,112-131,186-203,210-232,237-259
Sequence
Target Sequence 
>C. ELEGANS:178931_S_AT
tctgacaattgttctctatctcttcttttgtaaattcctgatcccaaatcctgcaaatgc
aaaagaacatgcaagaacgatttttactttattcattttcgcgtatgctttcactcctgt
gatacgaactttaactacttcaataagtacggatacaatttactctacatctatcatcac
tgccatttttagttgtttctttcatgattatggtgtcaaagccccagttgtatcatatcc
aacatccgtcagcactggcctcagctctgctattttcttgctctctcgattggaaggaga
tactccaaccctacttctacttgtcgttgccttcacattgcatgcatatggagctgaatt
caggaatcgtatattccacgtgtatccttgcctttcctcgacaatattctgttttctgtc
attattttccatctattgcatctctgatttctcacttgaactttcaatctgttttgctct
tctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTGACAATTGTTCTCTATCTCTTC 622 607 267 Antisense
GTAAATTCCTGATCCCAAATCCTGC 152 459 296 Antisense
TTCATTTTCGCGTATGCTTTCACTC 436 689 358 Antisense
TCTACATCTATCATCACTGCCATTT 92 615 430 Antisense
ATTTTCTTGCTCTCTCGATTGGAAG 153 15 538 Antisense
TCGTTGCCTTCACATTGCATGCATA 85 605 590 Antisense
AATCGTATATTCCACGTGTATCCTT 233 169 631 Antisense
TGCCTTTCCTCGACAATATTCTGTT 560 583 655 Antisense
TATTTTCCATCTATTGCATCTCTGA 324 663 689 Antisense
GCATCTCTGATTTCTCACTTGAACT 682 309 704 Antisense
ACTTTCAATCTGTTTTGCTCTTCTC 172 103 726 Antisense
Sequence Source Affymetrix Proprietary Database