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[178926_s_at]

Description R148.6 /REP_DB=WormBase Gene ID /WP=CE12858 /TR=O17271 /GB=AAB71040.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 35      6grp_total > 62      4grp_total > 48      6grp_highest_sub > 03      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1953
1 2 3 4 range
A 1540.7
(-3)
P 		3188
(2.1)
P 		3493.9
(3)
P 		2237.1
(-0.9)
P 		1953
B 1742
(-3)
P 		2715.4
(0.5)
P 		3420.7
(3)
P 		2076.2
(-1.8)
P 		1679
C 2330.5
(-3)
P 		2818.7
(1.3)
P 		3019.5
(3)
P 		2354.9
(-2.8)
P 		689
avr 1871.1
(-3) 		2907.4
(1.3) 		3311.4
(3) 		2222.7
(-1.5) 		1440

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3815
1 2 3 4 5 6 range
A 1995.7
(-2.5)
P 		3129.8
(-0.8)
P 		5523.2
(3)
P 		3539.8
(-0.1)
P 		2119.8
(-2.4)
P 		1707.8
(-3)
P 		3815
B 1791
(-3)
P 		3902.6
(1.5)
P 		4593.7
(3)
P 		3504.5
(0.7)
P 		2894.2
(-0.6)
P 		1860.9
(-2.9)
P 		2803
C 2060.9
(-2.4)
P 		3586.7
(1.7)
P 		4085.3
(3)
P 		4000.5
(2.8)
P 		2367.1
(-1.6)
P 		1846.9
(-3)
P 		2238
avr 1949.2
(-2.7) 		3539.7
(0.6) 		4734.1
(3) 		3681.6
(0.8) 		2460.4
(-1.7) 		1805.2
(-3) 		2929
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 725
1 2 3 4 range
A 561
(-0.6)
P 		692
(0.7)
P 		913.4
(3)
P 		327.5
(-3)
P 		586
B 555.5
(-1.1)
P 		670.7
(0.9)
P 		792.6
(3)
P 		442.4
(-3)
P 		350
C 771.5
(0.4)
P 		810.4
(0.8)
P 		1052.4
(3)
P 		397.1
(-3)
P 		655
avr 629.3
(-0.3) 		724.4
(0.8) 		919.5
(3) 		389
(-3) 		531

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 931
1 2 3 4 5 6 range
A 367.9
(-3)
P 		1113.1
(2.4)
P 		1202.8
(3)
P 		897.8
(0.8)
P 		639.7
(-1)
P 		415.8
(-2.7)
P 		835
B 623.2
(-3)
P 		1299
(3)
P 		1115.5
(1.4)
P 		928.2
(-0.3)
P 		1175.9
(1.9)
P 		693.9
(-2.4)
P 		676
C 763.9
(-1.2)
P 		1094.4
(3)
P 		1039.8
(2.3)
P 		804.5
(-0.7)
P 		766
(-1.1)
P 		618.3
(-3)
P 		476
avr 585
(-2.9) 		1168.8
(3) 		1119.4
(2.5) 		876.8
(0) 		860.5
(-0.1) 		576
(-3) 		593
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178926_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6487
Sequence Type Exemplar sequence
Representative Public ID R148.6 NCBI
Target Description R148.6 /REP_DB=WormBase Gene ID /WP=CE12858 /TR=O17271 /GB=AAB71040.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065270(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065270 NCBI Caenorhabditis elegans HE1 homologue family member (heh-1) (heh-1) mRNA, complete cds. 11/11 None
GENEFINDER00000030374 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3195577:3199785:-1 11/11 None
SNAP00000030368 ENSEMBL cdna:SNAP chromosome:CEL140:III:3198517:3199504:-1 11/11 None
R148.6.2 ENSEMBL cdna:known chromosome:CEL140:III:3198016:3199505:-1 gene:R148.6 11/11 None
R148.6.1 ENSEMBL cdna:known chromosome:CEL140:III:3198471:3199507:-1 gene:R148.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3198516-3200751(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol heh-1
Ensembl R148.6
Entrez Gene 175426 Entrez gene
SwissProt O17271 EMBL-EBI
WormBase CE31057 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71041 Hypothetical protein R148.7 [Caenorhabditis elegans] ref|NP_497670.1| R148.7 [Caenorhabditis elegans] 5.0E-98
blast AAB71040 He1 homologue protein 1 [Caenorhabditis elegans] ref|NP_497671.2| HE1 homologue family member (heh-1) [Caenorhabditis elegans] sp|O17271|HEH1_CAEEL Putative protein heh-1 precursor 1.0E-78
blast AAB71040 He1 homologue protein 1 [Caenorhabditis elegans] ref|NP_497671.2| HE1 homologue family member (heh-1) [Caenorhabditis elegans] sp|O17271|HEH1_CAEEL Putative protein heh-1 precursor 2.0E-71
blast CAE72967 Hypothetical protein CBG20304 [Caenorhabditis briggsae] 4.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR003172 EMBL-EBI E1 protein and Def2/Der2 allergen 3.0E-38
Pfam IPR003172 EMBL-EBI E1 protein and Def2/Der2 allergen 9.8E-33
Sequence
Target Sequence 
>C. ELEGANS:178926_S_AT
aagaccgtaattttcctggcattgcttggcctggccgccgccgagttcattgagatcggc
tacaaggtgtgcaaatccgacggtaccgtatcccaagtaaaagccgacggatgtgagctc
acagtgaaggacgggaagaaagtttgcctgttcaagaaaggaagccggccaattattcaa
atcgcgttcaaaccaagcaaggacaccgacaaactgaagacctccgtgcgcgctaaggtt
ggcggcagtgctatggttgacttcccacaaactaactcggatgcttgtacttatggtgta
aaatgcccagtttccgccggcgaaaatcaaatcttcgaacaatcgatctcgatcaccgag
aatcatccagcaggggaagttattcaagtcaattggcagcttactcgtcccgattctgga
aaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGACCGTAATTTTCCTGGCATTGC 586 151 112 Antisense
CCGCCGCCGAGTTCATTGAGATCGG 444 261 146 Antisense
GCAAATCCGACGGTACCGTATCCCA 615 315 182 Antisense
AAAGCCGACGGATGTGAGCTCACAG 41 125 211 Antisense
AGCCGGCCAATTATTCAAATCGCGT 217 75 274 Antisense
ACACCGACAAACTGAAGACCTCCGT 87 107 314 Antisense
TCCGTGCGCGCTAAGGTTGGCGGCA 1 595 334 Antisense
GTGCTATGGTTGACTTCCCACAAAC 675 479 359 Antisense
AACTCGGATGCTTGTACTTATGGTG 631 139 385 Antisense
TCACCGAGAATCATCCAGCAGGGGA 133 627 464 Antisense
CTTACTCGTCCCGATTCTGGAAAAG 633 219 511 Antisense
Sequence Source Affymetrix Proprietary Database